Cargando…
Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug
The aims of this work were to develop a processable, electrospun formulation of a model biopharmaceutical drug, β-galactosidase, and to demonstrate that higher production rates of biopharmaceutical-containing fibers can be achieved by using high-speed electrospinning compared to traditional electros...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680794/ https://www.ncbi.nlm.nih.gov/pubmed/31336743 http://dx.doi.org/10.3390/pharmaceutics11070329 |
_version_ | 1783441582921351168 |
---|---|
author | Vass, Panna Hirsch, Edit Kóczián, Rita Démuth, Balázs Farkas, Attila Fehér, Csaba Szabó, Edina Németh, Áron Andersen, Sune K. Vigh, Tamás Verreck, Geert Csontos, István Marosi, György Nagy, Zsombor K. |
author_facet | Vass, Panna Hirsch, Edit Kóczián, Rita Démuth, Balázs Farkas, Attila Fehér, Csaba Szabó, Edina Németh, Áron Andersen, Sune K. Vigh, Tamás Verreck, Geert Csontos, István Marosi, György Nagy, Zsombor K. |
author_sort | Vass, Panna |
collection | PubMed |
description | The aims of this work were to develop a processable, electrospun formulation of a model biopharmaceutical drug, β-galactosidase, and to demonstrate that higher production rates of biopharmaceutical-containing fibers can be achieved by using high-speed electrospinning compared to traditional electrospinning techniques. An aqueous solution of 7.6 w/w% polyvinyl alcohol, 0.6 w/w% polyethylene oxide, 9.9 w/w% mannitol, and 5.4 w/w% β-galactosidase was successfully electrospun with a 30 mL/h feeding rate, which is about 30 times higher than the feeding rate usually attained with single-needle electrospinning. According to X-ray diffraction measurements, polyvinyl alcohol, polyethylene oxide, and β-galactosidase were in an amorphous state in the fibers, whereas mannitol was crystalline (δ-polymorph). The presence of crystalline mannitol and the low water content enabled appropriate grinding of the fibrous sample without secondary drying. The ground powder was mixed with excipients commonly used during the preparation of pharmaceutical tablets and was successfully compressed into tablets. β-galactosidase remained stable during each of the processing steps (electrospinning, grinding, and tableting) and after one year of storage at room temperature in the tablets. The obtained results demonstrate that high-speed electrospinning is a viable alternative to traditional biopharmaceutical drying methods, especially for heat sensitive molecules, and tablet formulation is achievable from the electrospun material prepared this way. |
format | Online Article Text |
id | pubmed-6680794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66807942019-08-09 Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug Vass, Panna Hirsch, Edit Kóczián, Rita Démuth, Balázs Farkas, Attila Fehér, Csaba Szabó, Edina Németh, Áron Andersen, Sune K. Vigh, Tamás Verreck, Geert Csontos, István Marosi, György Nagy, Zsombor K. Pharmaceutics Article The aims of this work were to develop a processable, electrospun formulation of a model biopharmaceutical drug, β-galactosidase, and to demonstrate that higher production rates of biopharmaceutical-containing fibers can be achieved by using high-speed electrospinning compared to traditional electrospinning techniques. An aqueous solution of 7.6 w/w% polyvinyl alcohol, 0.6 w/w% polyethylene oxide, 9.9 w/w% mannitol, and 5.4 w/w% β-galactosidase was successfully electrospun with a 30 mL/h feeding rate, which is about 30 times higher than the feeding rate usually attained with single-needle electrospinning. According to X-ray diffraction measurements, polyvinyl alcohol, polyethylene oxide, and β-galactosidase were in an amorphous state in the fibers, whereas mannitol was crystalline (δ-polymorph). The presence of crystalline mannitol and the low water content enabled appropriate grinding of the fibrous sample without secondary drying. The ground powder was mixed with excipients commonly used during the preparation of pharmaceutical tablets and was successfully compressed into tablets. β-galactosidase remained stable during each of the processing steps (electrospinning, grinding, and tableting) and after one year of storage at room temperature in the tablets. The obtained results demonstrate that high-speed electrospinning is a viable alternative to traditional biopharmaceutical drying methods, especially for heat sensitive molecules, and tablet formulation is achievable from the electrospun material prepared this way. MDPI 2019-07-11 /pmc/articles/PMC6680794/ /pubmed/31336743 http://dx.doi.org/10.3390/pharmaceutics11070329 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vass, Panna Hirsch, Edit Kóczián, Rita Démuth, Balázs Farkas, Attila Fehér, Csaba Szabó, Edina Németh, Áron Andersen, Sune K. Vigh, Tamás Verreck, Geert Csontos, István Marosi, György Nagy, Zsombor K. Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug |
title | Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug |
title_full | Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug |
title_fullStr | Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug |
title_full_unstemmed | Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug |
title_short | Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug |
title_sort | scaled-up production and tableting of grindable electrospun fibers containing a protein-type drug |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680794/ https://www.ncbi.nlm.nih.gov/pubmed/31336743 http://dx.doi.org/10.3390/pharmaceutics11070329 |
work_keys_str_mv | AT vasspanna scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT hirschedit scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT koczianrita scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT demuthbalazs scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT farkasattila scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT fehercsaba scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT szaboedina scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT nemetharon scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT andersensunek scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT vightamas scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT verreckgeert scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT csontosistvan scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT marosigyorgy scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug AT nagyzsombork scaledupproductionandtabletingofgrindableelectrospunfiberscontainingaproteintypedrug |