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Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China

As a traditional natural medicine for treating many kinds of diseases, Gnetum parvifolium showed apparent inhibition on xanthine oxidase (XO). In this study, ultrafiltration combined with liquid chromatography-mass spectrometry (LC-MS) is used for the screening of XO inhibitors from Gnetum parvifoli...

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Autores principales: Tang, Xiaosheng, Tang, Ping, Ma, Lei, Liu, Liangliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680845/
https://www.ncbi.nlm.nih.gov/pubmed/31340570
http://dx.doi.org/10.3390/molecules24142671
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author Tang, Xiaosheng
Tang, Ping
Ma, Lei
Liu, Liangliang
author_facet Tang, Xiaosheng
Tang, Ping
Ma, Lei
Liu, Liangliang
author_sort Tang, Xiaosheng
collection PubMed
description As a traditional natural medicine for treating many kinds of diseases, Gnetum parvifolium showed apparent inhibition on xanthine oxidase (XO). In this study, ultrafiltration combined with liquid chromatography-mass spectrometry (LC-MS) is used for the screening of XO inhibitors from Gnetum parvifolium. Their antioxidation, XO inhibition, and enzymic kinetic parameters are also determined. Finally, piceatannol (1), rhaponiticin (2), resveratrol (3), and isorhapontigenin (4) are screened out and identified as XO inhibitors from the extract of Gnetum parvifolium. Four inhibitors show better inhibition than allopurinol and good radical scavenging abilities. However, the antioxidant activities are weaker than ascorbic acid. The kinetic parameters illustrate the inhibition mode of XO by piceatannol is competitive type, while the inhibition modes for rhaponiticin, resveratrol and isorhapontigenin are uncompetitive types. In order to evaluate the difference among samples obtained in China, the amounts of four inhibitors and related activities in 20 samples are assessed and analyzed by partial least squares analysis. The results indicate piceatannol contribute the highest coefficients in three kinds of activities. Based on these findings, more comprehensive research on pharmaceutical and biochemical activities of these four XO inhibitors could be conducted in future.
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spelling pubmed-66808452019-08-09 Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China Tang, Xiaosheng Tang, Ping Ma, Lei Liu, Liangliang Molecules Article As a traditional natural medicine for treating many kinds of diseases, Gnetum parvifolium showed apparent inhibition on xanthine oxidase (XO). In this study, ultrafiltration combined with liquid chromatography-mass spectrometry (LC-MS) is used for the screening of XO inhibitors from Gnetum parvifolium. Their antioxidation, XO inhibition, and enzymic kinetic parameters are also determined. Finally, piceatannol (1), rhaponiticin (2), resveratrol (3), and isorhapontigenin (4) are screened out and identified as XO inhibitors from the extract of Gnetum parvifolium. Four inhibitors show better inhibition than allopurinol and good radical scavenging abilities. However, the antioxidant activities are weaker than ascorbic acid. The kinetic parameters illustrate the inhibition mode of XO by piceatannol is competitive type, while the inhibition modes for rhaponiticin, resveratrol and isorhapontigenin are uncompetitive types. In order to evaluate the difference among samples obtained in China, the amounts of four inhibitors and related activities in 20 samples are assessed and analyzed by partial least squares analysis. The results indicate piceatannol contribute the highest coefficients in three kinds of activities. Based on these findings, more comprehensive research on pharmaceutical and biochemical activities of these four XO inhibitors could be conducted in future. MDPI 2019-07-23 /pmc/articles/PMC6680845/ /pubmed/31340570 http://dx.doi.org/10.3390/molecules24142671 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Xiaosheng
Tang, Ping
Ma, Lei
Liu, Liangliang
Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China
title Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China
title_full Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China
title_fullStr Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China
title_full_unstemmed Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China
title_short Screening and Evaluation of Xanthine Oxidase Inhibitors from Gnetum parvifolium in China
title_sort screening and evaluation of xanthine oxidase inhibitors from gnetum parvifolium in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680845/
https://www.ncbi.nlm.nih.gov/pubmed/31340570
http://dx.doi.org/10.3390/molecules24142671
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