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Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)

Conductive biopolymers, an important class of functional materials, have received attention in various fields because of their unique electrical, optical, and physical properties. In this study, the polymerization of heme into hemozoin was carried out in an in vitro system by the newly developed hem...

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Autores principales: Lee, Ju Hun, Kim, Hyeong Ryeol, Lee, Ja Hyun, Lee, Soo Kweon, Chun, Youngsang, Han, Sung Ok, Yoo, Hah Young, Park, Chulhwan, Kim, Seung Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680884/
https://www.ncbi.nlm.nih.gov/pubmed/31288462
http://dx.doi.org/10.3390/polym11071162
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author Lee, Ju Hun
Kim, Hyeong Ryeol
Lee, Ja Hyun
Lee, Soo Kweon
Chun, Youngsang
Han, Sung Ok
Yoo, Hah Young
Park, Chulhwan
Kim, Seung Wook
author_facet Lee, Ju Hun
Kim, Hyeong Ryeol
Lee, Ja Hyun
Lee, Soo Kweon
Chun, Youngsang
Han, Sung Ok
Yoo, Hah Young
Park, Chulhwan
Kim, Seung Wook
author_sort Lee, Ju Hun
collection PubMed
description Conductive biopolymers, an important class of functional materials, have received attention in various fields because of their unique electrical, optical, and physical properties. In this study, the polymerization of heme into hemozoin was carried out in an in vitro system by the newly developed heme polymerase (histidine-rich protein 2 (HRP-II)). The HRP-II was produced by recombinant E. coli BL21 from the Plasmodium falciparum gene. To improve the hemozoin production, the reaction conditions on the polymerization were investigated and the maximum production was achieved after about 790 μM at 34 °C with 200 rpm for 24 h. As a result, the production was improved about two-fold according to the stepwise optimization in an in vitro system. The produced hemozoin was qualitatively analyzed using the Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM). Finally, it was confirmed that the enzymatically polymerized hemozoin had similar physical properties to chemically synthesized hemozoin. These results could represent a significant potential for nano-biotechnology applications, and also provide guidance in research related to hemozoin utilization.
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spelling pubmed-66808842019-08-09 Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII) Lee, Ju Hun Kim, Hyeong Ryeol Lee, Ja Hyun Lee, Soo Kweon Chun, Youngsang Han, Sung Ok Yoo, Hah Young Park, Chulhwan Kim, Seung Wook Polymers (Basel) Article Conductive biopolymers, an important class of functional materials, have received attention in various fields because of their unique electrical, optical, and physical properties. In this study, the polymerization of heme into hemozoin was carried out in an in vitro system by the newly developed heme polymerase (histidine-rich protein 2 (HRP-II)). The HRP-II was produced by recombinant E. coli BL21 from the Plasmodium falciparum gene. To improve the hemozoin production, the reaction conditions on the polymerization were investigated and the maximum production was achieved after about 790 μM at 34 °C with 200 rpm for 24 h. As a result, the production was improved about two-fold according to the stepwise optimization in an in vitro system. The produced hemozoin was qualitatively analyzed using the Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM). Finally, it was confirmed that the enzymatically polymerized hemozoin had similar physical properties to chemically synthesized hemozoin. These results could represent a significant potential for nano-biotechnology applications, and also provide guidance in research related to hemozoin utilization. MDPI 2019-07-08 /pmc/articles/PMC6680884/ /pubmed/31288462 http://dx.doi.org/10.3390/polym11071162 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Ju Hun
Kim, Hyeong Ryeol
Lee, Ja Hyun
Lee, Soo Kweon
Chun, Youngsang
Han, Sung Ok
Yoo, Hah Young
Park, Chulhwan
Kim, Seung Wook
Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
title Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
title_full Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
title_fullStr Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
title_full_unstemmed Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
title_short Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
title_sort enhanced in-vitro hemozoin polymerization by optimized process using histidine-rich protein ii (hrpii)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680884/
https://www.ncbi.nlm.nih.gov/pubmed/31288462
http://dx.doi.org/10.3390/polym11071162
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