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Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology

Optimization of infrared-assisted extraction was conducted using Response Surface Methodology (RSM) in order to intensify polyphenol recovery from olive leaves. The extraction efficiency using Ired-Irrad(®), a newly-patented infrared apparatus (IR), was compared to water bath (WB) conventional extra...

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Autores principales: Abi-Khattar, Anna-Maria, Rajha, Hiba N., Abdel-Massih, Roula M., Maroun, Richard G., Louka, Nicolas, Debs, Espérance
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680986/
https://www.ncbi.nlm.nih.gov/pubmed/31323872
http://dx.doi.org/10.3390/antiox8070227
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author Abi-Khattar, Anna-Maria
Rajha, Hiba N.
Abdel-Massih, Roula M.
Maroun, Richard G.
Louka, Nicolas
Debs, Espérance
author_facet Abi-Khattar, Anna-Maria
Rajha, Hiba N.
Abdel-Massih, Roula M.
Maroun, Richard G.
Louka, Nicolas
Debs, Espérance
author_sort Abi-Khattar, Anna-Maria
collection PubMed
description Optimization of infrared-assisted extraction was conducted using Response Surface Methodology (RSM) in order to intensify polyphenol recovery from olive leaves. The extraction efficiency using Ired-Irrad(®), a newly-patented infrared apparatus (IR), was compared to water bath (WB) conventional extraction. Under optimal conditions, as suggested by the model and confirmed experimentally, the total phenolic content yield was enhanced by more than 30% using IR as contrasted to WB, which even required 27% more ethanol consumption. High Performance Liquid Chromatography analyses quantified the two major phenolic compounds of the leaves: Oleuropein and hydroxytyrosol, which were both intensified by 18% and 21%, respectively. IR extracts increased the antiradical activity by 25% and the antioxidant capacity by 51% compared to WB extracts. On the other hand, extracts of olive leaves obtained by both techniques exhibited equal effects regarding the inhibition of 20 strains of Staphylococcus aureus, with a minimum inhibitory concentration (MIC) varying between 3.125 and 12.5 mg/mL. Similarly, both extracts inhibited Aflatoxin B1 (AFB1) secretion by Aspergillus flavus, with no growth inhibition of the fungus. Finally, optimization using RSM allowed us to suggest other IR operating conditions aiming at significantly reducing the consumption of energy and solvent, while maintaining similar quantity and quality of phenolic compounds as what is optimally obtained using WB.
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spelling pubmed-66809862019-08-09 Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology Abi-Khattar, Anna-Maria Rajha, Hiba N. Abdel-Massih, Roula M. Maroun, Richard G. Louka, Nicolas Debs, Espérance Antioxidants (Basel) Article Optimization of infrared-assisted extraction was conducted using Response Surface Methodology (RSM) in order to intensify polyphenol recovery from olive leaves. The extraction efficiency using Ired-Irrad(®), a newly-patented infrared apparatus (IR), was compared to water bath (WB) conventional extraction. Under optimal conditions, as suggested by the model and confirmed experimentally, the total phenolic content yield was enhanced by more than 30% using IR as contrasted to WB, which even required 27% more ethanol consumption. High Performance Liquid Chromatography analyses quantified the two major phenolic compounds of the leaves: Oleuropein and hydroxytyrosol, which were both intensified by 18% and 21%, respectively. IR extracts increased the antiradical activity by 25% and the antioxidant capacity by 51% compared to WB extracts. On the other hand, extracts of olive leaves obtained by both techniques exhibited equal effects regarding the inhibition of 20 strains of Staphylococcus aureus, with a minimum inhibitory concentration (MIC) varying between 3.125 and 12.5 mg/mL. Similarly, both extracts inhibited Aflatoxin B1 (AFB1) secretion by Aspergillus flavus, with no growth inhibition of the fungus. Finally, optimization using RSM allowed us to suggest other IR operating conditions aiming at significantly reducing the consumption of energy and solvent, while maintaining similar quantity and quality of phenolic compounds as what is optimally obtained using WB. MDPI 2019-07-18 /pmc/articles/PMC6680986/ /pubmed/31323872 http://dx.doi.org/10.3390/antiox8070227 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abi-Khattar, Anna-Maria
Rajha, Hiba N.
Abdel-Massih, Roula M.
Maroun, Richard G.
Louka, Nicolas
Debs, Espérance
Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology
title Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology
title_full Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology
title_fullStr Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology
title_full_unstemmed Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology
title_short Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad(®), an Environmentally-Friendly Innovative Technology
title_sort intensification of polyphenol extraction from olive leaves using ired-irrad(®), an environmentally-friendly innovative technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680986/
https://www.ncbi.nlm.nih.gov/pubmed/31323872
http://dx.doi.org/10.3390/antiox8070227
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