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Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing

Cutaneous wounds represent a major issue in medical care, with approximately 300 million chronic and 100 million traumatic wound patients worldwide, and microbial infections slow the healing process. The aim of this work was to develop electrospun scaffolds loaded with silver nanoparticles (AgNPs) t...

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Autores principales: Sandri, Giuseppina, Miele, Dalila, Faccendini, Angela, Bonferoni, Maria Cristina, Rossi, Silvia, Grisoli, Pietro, Taglietti, Angelo, Ruggeri, Marco, Bruni, Giovanna, Vigani, Barbara, Ferrari, Franca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680995/
https://www.ncbi.nlm.nih.gov/pubmed/31330974
http://dx.doi.org/10.3390/polym11071207
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author Sandri, Giuseppina
Miele, Dalila
Faccendini, Angela
Bonferoni, Maria Cristina
Rossi, Silvia
Grisoli, Pietro
Taglietti, Angelo
Ruggeri, Marco
Bruni, Giovanna
Vigani, Barbara
Ferrari, Franca
author_facet Sandri, Giuseppina
Miele, Dalila
Faccendini, Angela
Bonferoni, Maria Cristina
Rossi, Silvia
Grisoli, Pietro
Taglietti, Angelo
Ruggeri, Marco
Bruni, Giovanna
Vigani, Barbara
Ferrari, Franca
author_sort Sandri, Giuseppina
collection PubMed
description Cutaneous wounds represent a major issue in medical care, with approximately 300 million chronic and 100 million traumatic wound patients worldwide, and microbial infections slow the healing process. The aim of this work was to develop electrospun scaffolds loaded with silver nanoparticles (AgNPs) to enhance cutaneous healing, preventing wound infections. AgNPs were directly added to polymeric blends based on chitosan (CH) and pullulan (PUL) with hyaluronic acid (HA) or chondroitin sulfate (CS) to be electrospun obtaining nanofibrous scaffolds. Moreover, a scaffold based on CH and PUL and loaded with AgNPs was prepared as a comparison. The scaffolds were characterized by chemico–physical properties, enzymatic degradation, biocompatibility, and antimicrobial properties. All the scaffolds were based on nanofibers (diameters about 500 nm) and the presence of AgNPs was evidenced by TEM and did not modify their morphology. The scaffold degradation was proven by means of lysozyme. Moreover, the AgNPs loaded scaffolds were characterized by a good propensity to promote fibroblast proliferation, avoiding the toxic effect of silver. Furthermore, scaffolds preserved AgNP antimicrobial properties, although silver was entrapped into nanofibers. Chitosan/chondroitin sulfate scaffold loaded with AgNPs demonstrated promotion of fibroblast proliferation and to possess antimicrobial properties, thus representing an interesting tool for the treatment of chronic wounds.
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spelling pubmed-66809952019-08-09 Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing Sandri, Giuseppina Miele, Dalila Faccendini, Angela Bonferoni, Maria Cristina Rossi, Silvia Grisoli, Pietro Taglietti, Angelo Ruggeri, Marco Bruni, Giovanna Vigani, Barbara Ferrari, Franca Polymers (Basel) Article Cutaneous wounds represent a major issue in medical care, with approximately 300 million chronic and 100 million traumatic wound patients worldwide, and microbial infections slow the healing process. The aim of this work was to develop electrospun scaffolds loaded with silver nanoparticles (AgNPs) to enhance cutaneous healing, preventing wound infections. AgNPs were directly added to polymeric blends based on chitosan (CH) and pullulan (PUL) with hyaluronic acid (HA) or chondroitin sulfate (CS) to be electrospun obtaining nanofibrous scaffolds. Moreover, a scaffold based on CH and PUL and loaded with AgNPs was prepared as a comparison. The scaffolds were characterized by chemico–physical properties, enzymatic degradation, biocompatibility, and antimicrobial properties. All the scaffolds were based on nanofibers (diameters about 500 nm) and the presence of AgNPs was evidenced by TEM and did not modify their morphology. The scaffold degradation was proven by means of lysozyme. Moreover, the AgNPs loaded scaffolds were characterized by a good propensity to promote fibroblast proliferation, avoiding the toxic effect of silver. Furthermore, scaffolds preserved AgNP antimicrobial properties, although silver was entrapped into nanofibers. Chitosan/chondroitin sulfate scaffold loaded with AgNPs demonstrated promotion of fibroblast proliferation and to possess antimicrobial properties, thus representing an interesting tool for the treatment of chronic wounds. MDPI 2019-07-19 /pmc/articles/PMC6680995/ /pubmed/31330974 http://dx.doi.org/10.3390/polym11071207 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sandri, Giuseppina
Miele, Dalila
Faccendini, Angela
Bonferoni, Maria Cristina
Rossi, Silvia
Grisoli, Pietro
Taglietti, Angelo
Ruggeri, Marco
Bruni, Giovanna
Vigani, Barbara
Ferrari, Franca
Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing
title Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing
title_full Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing
title_fullStr Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing
title_full_unstemmed Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing
title_short Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing
title_sort chitosan/glycosaminoglycan scaffolds: the role of silver nanoparticles to control microbial infections in wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680995/
https://www.ncbi.nlm.nih.gov/pubmed/31330974
http://dx.doi.org/10.3390/polym11071207
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