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Dedicated Setup for the Photoconversion of Fluorescent Dyes for Functional Electron Microscopy

Here, we describe a cost-effective setup for targeted photoconversion of fluorescent signals into electron dense ones. This approach has offered invaluable insights in the morphology and function of fine neuronal structures. The technique relies on the localized oxidation of diaminobenzidine (DAB) m...

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Detalles Bibliográficos
Autores principales: Dobson, Katharine L., Howe, Carmel L., Nishimura, Yuri, Marra, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681119/
https://www.ncbi.nlm.nih.gov/pubmed/31417358
http://dx.doi.org/10.3389/fncel.2019.00312