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Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species
Two sibling weevil species, Pissodes strobi Peck and P. nemorensis Germar (Coleoptera: Curculionidae), can form reduced-fitness hybrids in the laboratory, but neither their premating isolation mechanisms nor mating behaviors are well-understood. Cuticular hydrocarbons (CHCs) have been reported as cr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681335/ https://www.ncbi.nlm.nih.gov/pubmed/31340524 http://dx.doi.org/10.3390/insects10070217 |
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author | Chen, Ruixu Xu, Tian Hao, Dejun Teale, Stephen A. |
author_facet | Chen, Ruixu Xu, Tian Hao, Dejun Teale, Stephen A. |
author_sort | Chen, Ruixu |
collection | PubMed |
description | Two sibling weevil species, Pissodes strobi Peck and P. nemorensis Germar (Coleoptera: Curculionidae), can form reduced-fitness hybrids in the laboratory, but neither their premating isolation mechanisms nor mating behaviors are well-understood. Cuticular hydrocarbons (CHCs) have been reported as crucial chemical cues in mating recognition in many insects, including weevils, and, thus, may also mediate the mating behavior of P. strobi and P. nemorensis. We conducted a series of behavioral observations, bioassays, and chemical analyses to investigate the role of CHCs in their mating behavior. Copulation behavior of both species followed similar steps: approaching, mounting, tapping, aedeagus extrusion, and copulation. In P. strobi, hexane extraction significantly reduced the number of successful male copulations compared with freeze-killed females. Conversely, significantly fewer P. nemorensis males copulated with dead females compared with live females. No significant differences were detected among hexane-extracted, freeze-killed or recoated female carcasses to P. nemorensis. These findings suggested that female cuticular extracts contain important cues in mate recognition in P. strobi but not in P. nemorensis. We identified 21 CHCs from both species with variation in abundances between sexes and seasons. Discriminant analysis revealed incomplete overlap of CHC compositions in females of the two species in summer, when hybridization potentially occurs. |
format | Online Article Text |
id | pubmed-6681335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66813352019-08-09 Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species Chen, Ruixu Xu, Tian Hao, Dejun Teale, Stephen A. Insects Article Two sibling weevil species, Pissodes strobi Peck and P. nemorensis Germar (Coleoptera: Curculionidae), can form reduced-fitness hybrids in the laboratory, but neither their premating isolation mechanisms nor mating behaviors are well-understood. Cuticular hydrocarbons (CHCs) have been reported as crucial chemical cues in mating recognition in many insects, including weevils, and, thus, may also mediate the mating behavior of P. strobi and P. nemorensis. We conducted a series of behavioral observations, bioassays, and chemical analyses to investigate the role of CHCs in their mating behavior. Copulation behavior of both species followed similar steps: approaching, mounting, tapping, aedeagus extrusion, and copulation. In P. strobi, hexane extraction significantly reduced the number of successful male copulations compared with freeze-killed females. Conversely, significantly fewer P. nemorensis males copulated with dead females compared with live females. No significant differences were detected among hexane-extracted, freeze-killed or recoated female carcasses to P. nemorensis. These findings suggested that female cuticular extracts contain important cues in mate recognition in P. strobi but not in P. nemorensis. We identified 21 CHCs from both species with variation in abundances between sexes and seasons. Discriminant analysis revealed incomplete overlap of CHC compositions in females of the two species in summer, when hybridization potentially occurs. MDPI 2019-07-23 /pmc/articles/PMC6681335/ /pubmed/31340524 http://dx.doi.org/10.3390/insects10070217 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Ruixu Xu, Tian Hao, Dejun Teale, Stephen A. Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species |
title | Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species |
title_full | Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species |
title_fullStr | Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species |
title_full_unstemmed | Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species |
title_short | Cuticular Hydrocarbon Recognition in the Mating Behavior of Two Pissodes Species |
title_sort | cuticular hydrocarbon recognition in the mating behavior of two pissodes species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681335/ https://www.ncbi.nlm.nih.gov/pubmed/31340524 http://dx.doi.org/10.3390/insects10070217 |
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