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Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella

The bacterial flagellum is a large molecular complex composed of thousands of protein subunits for motility. The filamentous part of the flagellum, which is called the axial structure, consists of the filament, the hook, and the rods, with other minor components—the cap protein and the hook associat...

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Autores principales: Saijo-Hamano, Yumiko, Matsunami, Hideyuki, Namba, Keiichi, Imada, Katsumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681337/
https://www.ncbi.nlm.nih.gov/pubmed/31284631
http://dx.doi.org/10.3390/biom9070260
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author Saijo-Hamano, Yumiko
Matsunami, Hideyuki
Namba, Keiichi
Imada, Katsumi
author_facet Saijo-Hamano, Yumiko
Matsunami, Hideyuki
Namba, Keiichi
Imada, Katsumi
author_sort Saijo-Hamano, Yumiko
collection PubMed
description The bacterial flagellum is a large molecular complex composed of thousands of protein subunits for motility. The filamentous part of the flagellum, which is called the axial structure, consists of the filament, the hook, and the rods, with other minor components—the cap protein and the hook associated proteins. They share a common basic architecture of subunit arrangement, but each part shows quite distinct mechanical properties to achieve its specific function. The distal rod and the hook are helical assemblies of a single protein, FlgG and FlgE, respectively. They show a significant sequence similarity but have distinct mechanical characteristics. The rod is a rigid, straight cylinder, whereas the hook is a curved tube with high bending flexibility. Here, we report a structural model of the rod constructed by using the crystal structure of a core fragment of FlgG with a density map obtained previously by electron cryomicroscopy. Our structural model suggests that a segment called L-stretch plays a key role in achieving the distinct mechanical properties of the rod using a structurally similar component protein to that of the hook.
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spelling pubmed-66813372019-08-09 Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella Saijo-Hamano, Yumiko Matsunami, Hideyuki Namba, Keiichi Imada, Katsumi Biomolecules Article The bacterial flagellum is a large molecular complex composed of thousands of protein subunits for motility. The filamentous part of the flagellum, which is called the axial structure, consists of the filament, the hook, and the rods, with other minor components—the cap protein and the hook associated proteins. They share a common basic architecture of subunit arrangement, but each part shows quite distinct mechanical properties to achieve its specific function. The distal rod and the hook are helical assemblies of a single protein, FlgG and FlgE, respectively. They show a significant sequence similarity but have distinct mechanical characteristics. The rod is a rigid, straight cylinder, whereas the hook is a curved tube with high bending flexibility. Here, we report a structural model of the rod constructed by using the crystal structure of a core fragment of FlgG with a density map obtained previously by electron cryomicroscopy. Our structural model suggests that a segment called L-stretch plays a key role in achieving the distinct mechanical properties of the rod using a structurally similar component protein to that of the hook. MDPI 2019-07-07 /pmc/articles/PMC6681337/ /pubmed/31284631 http://dx.doi.org/10.3390/biom9070260 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saijo-Hamano, Yumiko
Matsunami, Hideyuki
Namba, Keiichi
Imada, Katsumi
Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella
title Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella
title_full Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella
title_fullStr Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella
title_full_unstemmed Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella
title_short Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella
title_sort architecture of the bacterial flagellar distal rod and hook of salmonella
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681337/
https://www.ncbi.nlm.nih.gov/pubmed/31284631
http://dx.doi.org/10.3390/biom9070260
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