Cargando…

Scaled‐up biotechnological production of individual betalains in a microbial system

The recent interest in plant pigment betalains as bioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel and efficient enzyme 4,5‐DOPA‐extradiol dioxygenase from Gluconacetobacter diazotrophicus in an expressi...

Descripción completa

Detalles Bibliográficos
Autores principales: Guerrero‐Rubio, María Alejandra, López‐Llorca, Rosalía, Henarejos‐Escudero, Paula, García‐Carmona, Francisco, Gandía‐Herrero, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681404/
https://www.ncbi.nlm.nih.gov/pubmed/31270958
http://dx.doi.org/10.1111/1751-7915.13452
_version_ 1783441724819898368
author Guerrero‐Rubio, María Alejandra
López‐Llorca, Rosalía
Henarejos‐Escudero, Paula
García‐Carmona, Francisco
Gandía‐Herrero, Fernando
author_facet Guerrero‐Rubio, María Alejandra
López‐Llorca, Rosalía
Henarejos‐Escudero, Paula
García‐Carmona, Francisco
Gandía‐Herrero, Fernando
author_sort Guerrero‐Rubio, María Alejandra
collection PubMed
description The recent interest in plant pigment betalains as bioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel and efficient enzyme 4,5‐DOPA‐extradiol dioxygenase from Gluconacetobacter diazotrophicus in an expression vector, and the subsequent heterologous expression in Escherichia coli cultures has led to the start‐up of a biotechnological production system of individual pigments. The aim of this study was to search for the optimal conditions for the production of betalamic acid in microbial factories and the scaled‐up obtention of the derived pigments. Four different betaxanthins and two betacyanins were obtained after the addition of non‐transformable amines and amino acids and their condensation with the betalamic acid produced by the dioxygenase. The scaled‐up obtention and purification of betalains improved the yields of the previous methodologies reaching quantities by up to 150 mg of pure compounds.
format Online
Article
Text
id pubmed-6681404
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-66814042019-08-12 Scaled‐up biotechnological production of individual betalains in a microbial system Guerrero‐Rubio, María Alejandra López‐Llorca, Rosalía Henarejos‐Escudero, Paula García‐Carmona, Francisco Gandía‐Herrero, Fernando Microb Biotechnol Research Articles The recent interest in plant pigment betalains as bioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel and efficient enzyme 4,5‐DOPA‐extradiol dioxygenase from Gluconacetobacter diazotrophicus in an expression vector, and the subsequent heterologous expression in Escherichia coli cultures has led to the start‐up of a biotechnological production system of individual pigments. The aim of this study was to search for the optimal conditions for the production of betalamic acid in microbial factories and the scaled‐up obtention of the derived pigments. Four different betaxanthins and two betacyanins were obtained after the addition of non‐transformable amines and amino acids and their condensation with the betalamic acid produced by the dioxygenase. The scaled‐up obtention and purification of betalains improved the yields of the previous methodologies reaching quantities by up to 150 mg of pure compounds. John Wiley and Sons Inc. 2019-07-04 /pmc/articles/PMC6681404/ /pubmed/31270958 http://dx.doi.org/10.1111/1751-7915.13452 Text en © 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Guerrero‐Rubio, María Alejandra
López‐Llorca, Rosalía
Henarejos‐Escudero, Paula
García‐Carmona, Francisco
Gandía‐Herrero, Fernando
Scaled‐up biotechnological production of individual betalains in a microbial system
title Scaled‐up biotechnological production of individual betalains in a microbial system
title_full Scaled‐up biotechnological production of individual betalains in a microbial system
title_fullStr Scaled‐up biotechnological production of individual betalains in a microbial system
title_full_unstemmed Scaled‐up biotechnological production of individual betalains in a microbial system
title_short Scaled‐up biotechnological production of individual betalains in a microbial system
title_sort scaled‐up biotechnological production of individual betalains in a microbial system
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681404/
https://www.ncbi.nlm.nih.gov/pubmed/31270958
http://dx.doi.org/10.1111/1751-7915.13452
work_keys_str_mv AT guerrerorubiomariaalejandra scaledupbiotechnologicalproductionofindividualbetalainsinamicrobialsystem
AT lopezllorcarosalia scaledupbiotechnologicalproductionofindividualbetalainsinamicrobialsystem
AT henarejosescuderopaula scaledupbiotechnologicalproductionofindividualbetalainsinamicrobialsystem
AT garciacarmonafrancisco scaledupbiotechnologicalproductionofindividualbetalainsinamicrobialsystem
AT gandiaherrerofernando scaledupbiotechnologicalproductionofindividualbetalainsinamicrobialsystem