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Scaled‐up biotechnological production of individual betalains in a microbial system
The recent interest in plant pigment betalains as bioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel and efficient enzyme 4,5‐DOPA‐extradiol dioxygenase from Gluconacetobacter diazotrophicus in an expressi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681404/ https://www.ncbi.nlm.nih.gov/pubmed/31270958 http://dx.doi.org/10.1111/1751-7915.13452 |
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author | Guerrero‐Rubio, María Alejandra López‐Llorca, Rosalía Henarejos‐Escudero, Paula García‐Carmona, Francisco Gandía‐Herrero, Fernando |
author_facet | Guerrero‐Rubio, María Alejandra López‐Llorca, Rosalía Henarejos‐Escudero, Paula García‐Carmona, Francisco Gandía‐Herrero, Fernando |
author_sort | Guerrero‐Rubio, María Alejandra |
collection | PubMed |
description | The recent interest in plant pigment betalains as bioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel and efficient enzyme 4,5‐DOPA‐extradiol dioxygenase from Gluconacetobacter diazotrophicus in an expression vector, and the subsequent heterologous expression in Escherichia coli cultures has led to the start‐up of a biotechnological production system of individual pigments. The aim of this study was to search for the optimal conditions for the production of betalamic acid in microbial factories and the scaled‐up obtention of the derived pigments. Four different betaxanthins and two betacyanins were obtained after the addition of non‐transformable amines and amino acids and their condensation with the betalamic acid produced by the dioxygenase. The scaled‐up obtention and purification of betalains improved the yields of the previous methodologies reaching quantities by up to 150 mg of pure compounds. |
format | Online Article Text |
id | pubmed-6681404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66814042019-08-12 Scaled‐up biotechnological production of individual betalains in a microbial system Guerrero‐Rubio, María Alejandra López‐Llorca, Rosalía Henarejos‐Escudero, Paula García‐Carmona, Francisco Gandía‐Herrero, Fernando Microb Biotechnol Research Articles The recent interest in plant pigment betalains as bioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel and efficient enzyme 4,5‐DOPA‐extradiol dioxygenase from Gluconacetobacter diazotrophicus in an expression vector, and the subsequent heterologous expression in Escherichia coli cultures has led to the start‐up of a biotechnological production system of individual pigments. The aim of this study was to search for the optimal conditions for the production of betalamic acid in microbial factories and the scaled‐up obtention of the derived pigments. Four different betaxanthins and two betacyanins were obtained after the addition of non‐transformable amines and amino acids and their condensation with the betalamic acid produced by the dioxygenase. The scaled‐up obtention and purification of betalains improved the yields of the previous methodologies reaching quantities by up to 150 mg of pure compounds. John Wiley and Sons Inc. 2019-07-04 /pmc/articles/PMC6681404/ /pubmed/31270958 http://dx.doi.org/10.1111/1751-7915.13452 Text en © 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Guerrero‐Rubio, María Alejandra López‐Llorca, Rosalía Henarejos‐Escudero, Paula García‐Carmona, Francisco Gandía‐Herrero, Fernando Scaled‐up biotechnological production of individual betalains in a microbial system |
title | Scaled‐up biotechnological production of individual betalains in a microbial system |
title_full | Scaled‐up biotechnological production of individual betalains in a microbial system |
title_fullStr | Scaled‐up biotechnological production of individual betalains in a microbial system |
title_full_unstemmed | Scaled‐up biotechnological production of individual betalains in a microbial system |
title_short | Scaled‐up biotechnological production of individual betalains in a microbial system |
title_sort | scaled‐up biotechnological production of individual betalains in a microbial system |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681404/ https://www.ncbi.nlm.nih.gov/pubmed/31270958 http://dx.doi.org/10.1111/1751-7915.13452 |
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