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Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model

The aim was to analyze histologically the bone repair in a mandibular osteotomy model with different gaps between the segments. Nine male rabbits who underwent osteotomies on the mandibular body were fixed with a 1.5 system plate and no bone graft; group 1 (2 mm gap between segments), group 2 (5 mm...

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Autores principales: Olate, Sergio, Vásquez, Bélgica, Sandoval, Cristian, Vasconcellos, Adriana, Alister, Juan Pablo, del Sol, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681602/
https://www.ncbi.nlm.nih.gov/pubmed/31428646
http://dx.doi.org/10.1155/2019/8043510
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author Olate, Sergio
Vásquez, Bélgica
Sandoval, Cristian
Vasconcellos, Adriana
Alister, Juan Pablo
del Sol, Mariano
author_facet Olate, Sergio
Vásquez, Bélgica
Sandoval, Cristian
Vasconcellos, Adriana
Alister, Juan Pablo
del Sol, Mariano
author_sort Olate, Sergio
collection PubMed
description The aim was to analyze histologically the bone repair in a mandibular osteotomy model with different gaps between the segments. Nine male rabbits who underwent osteotomies on the mandibular body were fixed with a 1.5 system plate and no bone graft; group 1 (2 mm gap between segments), group 2 (5 mm gap between segments), and group 3 (8 mm gap between segments) were included. After 8 weeks they were euthanized and the sample was processed for histological analysis. Group 1 showed advanced bone repair with cartilaginous tissue and cancellous bone, showing osteoblasts and type III collagenous fibers. In group 2, a more delayed ossification was observed, with an extensive area of peripheral ossifying cartilage and chondrocytes in greater number at the center of the defect; group 3 showed no evidence of ossification with fibrous tissue, a very low level of chondrocytes, and some bone sequestrate. We can conclude that, in this animal model, 2 or 5 mm gap in the osteotomy could be repaired as bone when fixation is used. The size of the gap is an important factor for the use of bone grafts considering endochondral ossification. This model can be used for graft analysis and related technologies.
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spelling pubmed-66816022019-08-19 Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model Olate, Sergio Vásquez, Bélgica Sandoval, Cristian Vasconcellos, Adriana Alister, Juan Pablo del Sol, Mariano Biomed Res Int Research Article The aim was to analyze histologically the bone repair in a mandibular osteotomy model with different gaps between the segments. Nine male rabbits who underwent osteotomies on the mandibular body were fixed with a 1.5 system plate and no bone graft; group 1 (2 mm gap between segments), group 2 (5 mm gap between segments), and group 3 (8 mm gap between segments) were included. After 8 weeks they were euthanized and the sample was processed for histological analysis. Group 1 showed advanced bone repair with cartilaginous tissue and cancellous bone, showing osteoblasts and type III collagenous fibers. In group 2, a more delayed ossification was observed, with an extensive area of peripheral ossifying cartilage and chondrocytes in greater number at the center of the defect; group 3 showed no evidence of ossification with fibrous tissue, a very low level of chondrocytes, and some bone sequestrate. We can conclude that, in this animal model, 2 or 5 mm gap in the osteotomy could be repaired as bone when fixation is used. The size of the gap is an important factor for the use of bone grafts considering endochondral ossification. This model can be used for graft analysis and related technologies. Hindawi 2019-07-24 /pmc/articles/PMC6681602/ /pubmed/31428646 http://dx.doi.org/10.1155/2019/8043510 Text en Copyright © 2019 Sergio Olate et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Olate, Sergio
Vásquez, Bélgica
Sandoval, Cristian
Vasconcellos, Adriana
Alister, Juan Pablo
del Sol, Mariano
Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model
title Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model
title_full Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model
title_fullStr Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model
title_full_unstemmed Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model
title_short Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model
title_sort histological analysis of bone repair in mandibular body osteotomy using internal fixation system in three different gaps without bone graft in an animal model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681602/
https://www.ncbi.nlm.nih.gov/pubmed/31428646
http://dx.doi.org/10.1155/2019/8043510
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