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Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion

Desiccation is one of the extremely stressful situations experienced by aquatic animals, and sea cucumber usually suffers from desiccation stress during transportation without water. The present study was conducted to evaluate the effect of desiccation and subsequent resubmersion on physiological st...

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Autores principales: Hou, Shiying, Jin, Zewei, Jiang, Wenwen, Chi, Liang, Xia, Bin, Chen, Jinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681796/
https://www.ncbi.nlm.nih.gov/pubmed/31396455
http://dx.doi.org/10.7717/peerj.7427
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author Hou, Shiying
Jin, Zewei
Jiang, Wenwen
Chi, Liang
Xia, Bin
Chen, Jinghua
author_facet Hou, Shiying
Jin, Zewei
Jiang, Wenwen
Chi, Liang
Xia, Bin
Chen, Jinghua
author_sort Hou, Shiying
collection PubMed
description Desiccation is one of the extremely stressful situations experienced by aquatic animals, and sea cucumber usually suffers from desiccation stress during transportation without water. The present study was conducted to evaluate the effect of desiccation and subsequent resubmersion on physiological stress, oxidative damage, antioxidant status and non-specific immune response of Apostichopus japonicus, providing valuable information on the health management of sea cucumber culturing. Control and desiccation groups were set up, and each group has three replicates. After 1, 3 and 6 h of desiccation, individuals were resubmersed in aerated seawater for a 24 h recovery in three batches, which were represented as D1, D3 and D6, respectively. The results showed that glucose level in coelomic fluid of sea cucumber significantly decreased after desiccation, whereas lactate, cortisol and osmolality showed remarkable ascending trends. Thereafter, all stress parameters gently recovered towards normal levels as control group during 24 h resubmersion. The prolonged desiccation at D6 treatment induced the significant increases of malondialdehyde (MDA) and reactive oxygen species (ROS) contents, as well as relatively lower superoxide dismutase (SOD) and catalase (CAT) activities. During the period of desiccation and subsequent resubmersion, sea cucumber adjusted antioxidant defense to reduce the concentrations of MDA and ROS as a strategy for protecting against oxidative damage. Desiccation also had significant effects on non-specific immune parameters (total coelomocytes counts, TCC; complement C3; total nitric oxide synthase, T-NOS; lysozyme, LSZ; alkaline phosphatase, AKP) of A. japonicus, which could be recovered to some extent during resubmersion. In conclusion, less than 6 h of desiccation did not induce irreparable damage to sea cucumber, and was recommended for handling and shipping live sea cucumbers.
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spelling pubmed-66817962019-08-08 Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion Hou, Shiying Jin, Zewei Jiang, Wenwen Chi, Liang Xia, Bin Chen, Jinghua PeerJ Aquaculture, Fisheries and Fish Science Desiccation is one of the extremely stressful situations experienced by aquatic animals, and sea cucumber usually suffers from desiccation stress during transportation without water. The present study was conducted to evaluate the effect of desiccation and subsequent resubmersion on physiological stress, oxidative damage, antioxidant status and non-specific immune response of Apostichopus japonicus, providing valuable information on the health management of sea cucumber culturing. Control and desiccation groups were set up, and each group has three replicates. After 1, 3 and 6 h of desiccation, individuals were resubmersed in aerated seawater for a 24 h recovery in three batches, which were represented as D1, D3 and D6, respectively. The results showed that glucose level in coelomic fluid of sea cucumber significantly decreased after desiccation, whereas lactate, cortisol and osmolality showed remarkable ascending trends. Thereafter, all stress parameters gently recovered towards normal levels as control group during 24 h resubmersion. The prolonged desiccation at D6 treatment induced the significant increases of malondialdehyde (MDA) and reactive oxygen species (ROS) contents, as well as relatively lower superoxide dismutase (SOD) and catalase (CAT) activities. During the period of desiccation and subsequent resubmersion, sea cucumber adjusted antioxidant defense to reduce the concentrations of MDA and ROS as a strategy for protecting against oxidative damage. Desiccation also had significant effects on non-specific immune parameters (total coelomocytes counts, TCC; complement C3; total nitric oxide synthase, T-NOS; lysozyme, LSZ; alkaline phosphatase, AKP) of A. japonicus, which could be recovered to some extent during resubmersion. In conclusion, less than 6 h of desiccation did not induce irreparable damage to sea cucumber, and was recommended for handling and shipping live sea cucumbers. PeerJ Inc. 2019-08-02 /pmc/articles/PMC6681796/ /pubmed/31396455 http://dx.doi.org/10.7717/peerj.7427 Text en ©2019 Hou et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Hou, Shiying
Jin, Zewei
Jiang, Wenwen
Chi, Liang
Xia, Bin
Chen, Jinghua
Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion
title Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion
title_full Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion
title_fullStr Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion
title_full_unstemmed Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion
title_short Physiological and immunological responses of sea cucumber Apostichopus japonicus during desiccation and subsequent resubmersion
title_sort physiological and immunological responses of sea cucumber apostichopus japonicus during desiccation and subsequent resubmersion
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681796/
https://www.ncbi.nlm.nih.gov/pubmed/31396455
http://dx.doi.org/10.7717/peerj.7427
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