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pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells

[Image: see text] We report the development of pH-labile ascorbic acid-coated magnetic nanocarriers (AMNCs) for effective delivery of the anticancer drug doxorubicin hydrochloride (DOX) to tumor cells. The uniqueness of this drug delivery system lies in the covalent conjugation of DOX through carbam...

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Autores principales: Gawali, Santosh L., Barick, Kanhu C., Shetake, Neena G., Rajan, Vasumathy, Pandey, Badri. N., Kumar, N. Naveen, Priyadarsini, K. Indira, Hassan, Puthusserickal A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682152/
https://www.ncbi.nlm.nih.gov/pubmed/31460279
http://dx.doi.org/10.1021/acsomega.9b01062
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author Gawali, Santosh L.
Barick, Kanhu C.
Shetake, Neena G.
Rajan, Vasumathy
Pandey, Badri. N.
Kumar, N. Naveen
Priyadarsini, K. Indira
Hassan, Puthusserickal A.
author_facet Gawali, Santosh L.
Barick, Kanhu C.
Shetake, Neena G.
Rajan, Vasumathy
Pandey, Badri. N.
Kumar, N. Naveen
Priyadarsini, K. Indira
Hassan, Puthusserickal A.
author_sort Gawali, Santosh L.
collection PubMed
description [Image: see text] We report the development of pH-labile ascorbic acid-coated magnetic nanocarriers (AMNCs) for effective delivery of the anticancer drug doxorubicin hydrochloride (DOX) to tumor cells. The uniqueness of this drug delivery system lies in the covalent conjugation of DOX through carbamate and hydrazone bonds, resulting in a slow and sustained drug release profile at different environmental acidities. X-ray diffraction and transmission electron microscopy analyses reveal the formation of crystalline single-phase Fe(3)O(4) nanoparticles with an average size of 10 nm. The changes in the interfacial characteristics of the nanocarriers and the presence of organic coatings are probed by infrared spectroscopy, dynamic light scattering, zeta potential, and thermogravimetric measurements. AMNCs show high colloidal stability in aqueous and cell culture media and possess good magnetic field responsivity and protein resistance characteristics. The drug-loaded nanocarriers exhibited sustained pH-triggered release of drug molecules in acidic mediums, substantial cellular internalization, and significant toxicity toward the proliferation of mouse skin fibrosarcoma (WEHI-164), human breast cancer (MCF-7), and human lung cancer (A549) cells. However, it showed significantly lower toxicity in human normal lung (WI26VA) cells. Overall, these results suggest a pH-sensitive drug release of nanoformulations, which showed selective toxicity to tumor than normal cells.
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spelling pubmed-66821522019-08-27 pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells Gawali, Santosh L. Barick, Kanhu C. Shetake, Neena G. Rajan, Vasumathy Pandey, Badri. N. Kumar, N. Naveen Priyadarsini, K. Indira Hassan, Puthusserickal A. ACS Omega [Image: see text] We report the development of pH-labile ascorbic acid-coated magnetic nanocarriers (AMNCs) for effective delivery of the anticancer drug doxorubicin hydrochloride (DOX) to tumor cells. The uniqueness of this drug delivery system lies in the covalent conjugation of DOX through carbamate and hydrazone bonds, resulting in a slow and sustained drug release profile at different environmental acidities. X-ray diffraction and transmission electron microscopy analyses reveal the formation of crystalline single-phase Fe(3)O(4) nanoparticles with an average size of 10 nm. The changes in the interfacial characteristics of the nanocarriers and the presence of organic coatings are probed by infrared spectroscopy, dynamic light scattering, zeta potential, and thermogravimetric measurements. AMNCs show high colloidal stability in aqueous and cell culture media and possess good magnetic field responsivity and protein resistance characteristics. The drug-loaded nanocarriers exhibited sustained pH-triggered release of drug molecules in acidic mediums, substantial cellular internalization, and significant toxicity toward the proliferation of mouse skin fibrosarcoma (WEHI-164), human breast cancer (MCF-7), and human lung cancer (A549) cells. However, it showed significantly lower toxicity in human normal lung (WI26VA) cells. Overall, these results suggest a pH-sensitive drug release of nanoformulations, which showed selective toxicity to tumor than normal cells. American Chemical Society 2019-07-05 /pmc/articles/PMC6682152/ /pubmed/31460279 http://dx.doi.org/10.1021/acsomega.9b01062 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Gawali, Santosh L.
Barick, Kanhu C.
Shetake, Neena G.
Rajan, Vasumathy
Pandey, Badri. N.
Kumar, N. Naveen
Priyadarsini, K. Indira
Hassan, Puthusserickal A.
pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells
title pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells
title_full pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells
title_fullStr pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells
title_full_unstemmed pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells
title_short pH-Labile Magnetic Nanocarriers for Intracellular Drug Delivery to Tumor Cells
title_sort ph-labile magnetic nanocarriers for intracellular drug delivery to tumor cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682152/
https://www.ncbi.nlm.nih.gov/pubmed/31460279
http://dx.doi.org/10.1021/acsomega.9b01062
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