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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability
The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682578/ https://www.ncbi.nlm.nih.gov/pubmed/31124019 http://dx.doi.org/10.1007/s10393-019-01414-6 |
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author | Lindauer, Alexa May, Tiffany Rios-Sotelo, Gabriela Sheets, Ciara Voyles, Jamie |
author_facet | Lindauer, Alexa May, Tiffany Rios-Sotelo, Gabriela Sheets, Ciara Voyles, Jamie |
author_sort | Lindauer, Alexa |
collection | PubMed |
description | The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis. |
format | Online Article Text |
id | pubmed-6682578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-66825782019-08-19 Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability Lindauer, Alexa May, Tiffany Rios-Sotelo, Gabriela Sheets, Ciara Voyles, Jamie Ecohealth Short Communication The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis. Springer US 2019-05-23 2019 /pmc/articles/PMC6682578/ /pubmed/31124019 http://dx.doi.org/10.1007/s10393-019-01414-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Short Communication Lindauer, Alexa May, Tiffany Rios-Sotelo, Gabriela Sheets, Ciara Voyles, Jamie Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability |
title | Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability |
title_full | Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability |
title_fullStr | Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability |
title_full_unstemmed | Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability |
title_short | Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability |
title_sort | quantifying batrachochytrium dendrobatidis and batrachochytrium salamandrivorans viability |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682578/ https://www.ncbi.nlm.nih.gov/pubmed/31124019 http://dx.doi.org/10.1007/s10393-019-01414-6 |
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