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Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy
Background: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild sub...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682795/ https://www.ncbi.nlm.nih.gov/pubmed/31267690 http://dx.doi.org/10.1002/vms3.181 |
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author | Tegtmeyer, Birthe Echelmeyer, Julia Pfankuche, Vanessa M. Puff, Christina Todt, Daniel Fischer, Nicole Durham, Andy Feige, Karsten Baumgärtner, Wolfgang Steinmann, Eike Cavalleri, Jessika‐M. V. |
author_facet | Tegtmeyer, Birthe Echelmeyer, Julia Pfankuche, Vanessa M. Puff, Christina Todt, Daniel Fischer, Nicole Durham, Andy Feige, Karsten Baumgärtner, Wolfgang Steinmann, Eike Cavalleri, Jessika‐M. V. |
author_sort | Tegtmeyer, Birthe |
collection | PubMed |
description | Background: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for EqHV to date. Objectives: EqHV can be involved in chronic liver diseases of horses. Methods: Biochemical parameters in serum samples were measured. Viral load was determined using qPCR. Next generation sequencing (NGS) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral RNA with fluorescent in situ‐hybridization. Results: The horse showed symptoms of severe hepatopathy and was chronically infected with EqHV. Viral RNA was detectable in the liver during disease. To rule out other infectious agents NGS was performed and showed the highest abundance for EqHV. The identified virus sequence was similar to other circulating equine hepaciviruses. Conclusions: EqHV can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation. |
format | Online Article Text |
id | pubmed-6682795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66827952019-08-12 Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy Tegtmeyer, Birthe Echelmeyer, Julia Pfankuche, Vanessa M. Puff, Christina Todt, Daniel Fischer, Nicole Durham, Andy Feige, Karsten Baumgärtner, Wolfgang Steinmann, Eike Cavalleri, Jessika‐M. V. Vet Med Sci Case Report Background: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for EqHV to date. Objectives: EqHV can be involved in chronic liver diseases of horses. Methods: Biochemical parameters in serum samples were measured. Viral load was determined using qPCR. Next generation sequencing (NGS) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral RNA with fluorescent in situ‐hybridization. Results: The horse showed symptoms of severe hepatopathy and was chronically infected with EqHV. Viral RNA was detectable in the liver during disease. To rule out other infectious agents NGS was performed and showed the highest abundance for EqHV. The identified virus sequence was similar to other circulating equine hepaciviruses. Conclusions: EqHV can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation. John Wiley and Sons Inc. 2019-07-03 /pmc/articles/PMC6682795/ /pubmed/31267690 http://dx.doi.org/10.1002/vms3.181 Text en © 2019 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Case Report Tegtmeyer, Birthe Echelmeyer, Julia Pfankuche, Vanessa M. Puff, Christina Todt, Daniel Fischer, Nicole Durham, Andy Feige, Karsten Baumgärtner, Wolfgang Steinmann, Eike Cavalleri, Jessika‐M. V. Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
title | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
title_full | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
title_fullStr | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
title_full_unstemmed | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
title_short | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
title_sort | chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682795/ https://www.ncbi.nlm.nih.gov/pubmed/31267690 http://dx.doi.org/10.1002/vms3.181 |
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