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Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration

We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omep...

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Autores principales: Fahey, Jed W., Wade, Kristina L., Stephenson, Katherine K., Panjwani, Anita A., Liu, Hua, Cornblatt, Grace, Cornblatt, Brian S., Ownby, Stacy L., Fuchs, Edward, Holtzclaw, Walter David, Cheskin, Lawrence J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682992/
https://www.ncbi.nlm.nih.gov/pubmed/31261930
http://dx.doi.org/10.3390/nu11071489
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author Fahey, Jed W.
Wade, Kristina L.
Stephenson, Katherine K.
Panjwani, Anita A.
Liu, Hua
Cornblatt, Grace
Cornblatt, Brian S.
Ownby, Stacy L.
Fuchs, Edward
Holtzclaw, Walter David
Cheskin, Lawrence J.
author_facet Fahey, Jed W.
Wade, Kristina L.
Stephenson, Katherine K.
Panjwani, Anita A.
Liu, Hua
Cornblatt, Grace
Cornblatt, Brian S.
Ownby, Stacy L.
Fuchs, Edward
Holtzclaw, Walter David
Cheskin, Lawrence J.
author_sort Fahey, Jed W.
collection PubMed
description We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis.
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spelling pubmed-66829922019-08-09 Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration Fahey, Jed W. Wade, Kristina L. Stephenson, Katherine K. Panjwani, Anita A. Liu, Hua Cornblatt, Grace Cornblatt, Brian S. Ownby, Stacy L. Fuchs, Edward Holtzclaw, Walter David Cheskin, Lawrence J. Nutrients Article We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis. MDPI 2019-06-29 /pmc/articles/PMC6682992/ /pubmed/31261930 http://dx.doi.org/10.3390/nu11071489 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fahey, Jed W.
Wade, Kristina L.
Stephenson, Katherine K.
Panjwani, Anita A.
Liu, Hua
Cornblatt, Grace
Cornblatt, Brian S.
Ownby, Stacy L.
Fuchs, Edward
Holtzclaw, Walter David
Cheskin, Lawrence J.
Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
title Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
title_full Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
title_fullStr Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
title_full_unstemmed Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
title_short Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
title_sort bioavailability of sulforaphane following ingestion of glucoraphanin-rich broccoli sprout and seed extracts with active myrosinase: a pilot study of the effects of proton pump inhibitor administration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682992/
https://www.ncbi.nlm.nih.gov/pubmed/31261930
http://dx.doi.org/10.3390/nu11071489
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