Cargando…
Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration
We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omep...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682992/ https://www.ncbi.nlm.nih.gov/pubmed/31261930 http://dx.doi.org/10.3390/nu11071489 |
_version_ | 1783441992975384576 |
---|---|
author | Fahey, Jed W. Wade, Kristina L. Stephenson, Katherine K. Panjwani, Anita A. Liu, Hua Cornblatt, Grace Cornblatt, Brian S. Ownby, Stacy L. Fuchs, Edward Holtzclaw, Walter David Cheskin, Lawrence J. |
author_facet | Fahey, Jed W. Wade, Kristina L. Stephenson, Katherine K. Panjwani, Anita A. Liu, Hua Cornblatt, Grace Cornblatt, Brian S. Ownby, Stacy L. Fuchs, Edward Holtzclaw, Walter David Cheskin, Lawrence J. |
author_sort | Fahey, Jed W. |
collection | PubMed |
description | We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis. |
format | Online Article Text |
id | pubmed-6682992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66829922019-08-09 Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration Fahey, Jed W. Wade, Kristina L. Stephenson, Katherine K. Panjwani, Anita A. Liu, Hua Cornblatt, Grace Cornblatt, Brian S. Ownby, Stacy L. Fuchs, Edward Holtzclaw, Walter David Cheskin, Lawrence J. Nutrients Article We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis. MDPI 2019-06-29 /pmc/articles/PMC6682992/ /pubmed/31261930 http://dx.doi.org/10.3390/nu11071489 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fahey, Jed W. Wade, Kristina L. Stephenson, Katherine K. Panjwani, Anita A. Liu, Hua Cornblatt, Grace Cornblatt, Brian S. Ownby, Stacy L. Fuchs, Edward Holtzclaw, Walter David Cheskin, Lawrence J. Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration |
title | Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration |
title_full | Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration |
title_fullStr | Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration |
title_full_unstemmed | Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration |
title_short | Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration |
title_sort | bioavailability of sulforaphane following ingestion of glucoraphanin-rich broccoli sprout and seed extracts with active myrosinase: a pilot study of the effects of proton pump inhibitor administration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682992/ https://www.ncbi.nlm.nih.gov/pubmed/31261930 http://dx.doi.org/10.3390/nu11071489 |
work_keys_str_mv | AT faheyjedw bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT wadekristinal bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT stephensonkatherinek bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT panjwanianitaa bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT liuhua bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT cornblattgrace bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT cornblattbrians bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT ownbystacyl bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT fuchsedward bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT holtzclawwalterdavid bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration AT cheskinlawrencej bioavailabilityofsulforaphanefollowingingestionofglucoraphaninrichbroccolisproutandseedextractswithactivemyrosinaseapilotstudyoftheeffectsofprotonpumpinhibitoradministration |