Cargando…
Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin +...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683036/ https://www.ncbi.nlm.nih.gov/pubmed/31315186 http://dx.doi.org/10.3390/nu11071610 |
_version_ | 1783442000504160256 |
---|---|
author | Santiago-López, Lourdes Hernández-Mendoza, Adrián Vallejo-Cordoba, Belinda Mata-Haro, Verónica Wall-Medrano, Abraham González-Córdova, Aarón F. |
author_facet | Santiago-López, Lourdes Hernández-Mendoza, Adrián Vallejo-Cordoba, Belinda Mata-Haro, Verónica Wall-Medrano, Abraham González-Córdova, Aarón F. |
author_sort | Santiago-López, Lourdes |
collection | PubMed |
description | The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice. |
format | Online Article Text |
id | pubmed-6683036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66830362019-08-09 Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study Santiago-López, Lourdes Hernández-Mendoza, Adrián Vallejo-Cordoba, Belinda Mata-Haro, Verónica Wall-Medrano, Abraham González-Córdova, Aarón F. Nutrients Article The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice. MDPI 2019-07-16 /pmc/articles/PMC6683036/ /pubmed/31315186 http://dx.doi.org/10.3390/nu11071610 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Santiago-López, Lourdes Hernández-Mendoza, Adrián Vallejo-Cordoba, Belinda Mata-Haro, Verónica Wall-Medrano, Abraham González-Córdova, Aarón F. Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study |
title | Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study |
title_full | Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study |
title_fullStr | Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study |
title_full_unstemmed | Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study |
title_short | Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study |
title_sort | milk fermented with lactobacillus fermentum ameliorates indomethacin-induced intestinal inflammation: an exploratory study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683036/ https://www.ncbi.nlm.nih.gov/pubmed/31315186 http://dx.doi.org/10.3390/nu11071610 |
work_keys_str_mv | AT santiagolopezlourdes milkfermentedwithlactobacillusfermentumamelioratesindomethacininducedintestinalinflammationanexploratorystudy AT hernandezmendozaadrian milkfermentedwithlactobacillusfermentumamelioratesindomethacininducedintestinalinflammationanexploratorystudy AT vallejocordobabelinda milkfermentedwithlactobacillusfermentumamelioratesindomethacininducedintestinalinflammationanexploratorystudy AT mataharoveronica milkfermentedwithlactobacillusfermentumamelioratesindomethacininducedintestinalinflammationanexploratorystudy AT wallmedranoabraham milkfermentedwithlactobacillusfermentumamelioratesindomethacininducedintestinalinflammationanexploratorystudy AT gonzalezcordovaaaronf milkfermentedwithlactobacillusfermentumamelioratesindomethacininducedintestinalinflammationanexploratorystudy |