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Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study

The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin +...

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Autores principales: Santiago-López, Lourdes, Hernández-Mendoza, Adrián, Vallejo-Cordoba, Belinda, Mata-Haro, Verónica, Wall-Medrano, Abraham, González-Córdova, Aarón F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683036/
https://www.ncbi.nlm.nih.gov/pubmed/31315186
http://dx.doi.org/10.3390/nu11071610
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author Santiago-López, Lourdes
Hernández-Mendoza, Adrián
Vallejo-Cordoba, Belinda
Mata-Haro, Verónica
Wall-Medrano, Abraham
González-Córdova, Aarón F.
author_facet Santiago-López, Lourdes
Hernández-Mendoza, Adrián
Vallejo-Cordoba, Belinda
Mata-Haro, Verónica
Wall-Medrano, Abraham
González-Córdova, Aarón F.
author_sort Santiago-López, Lourdes
collection PubMed
description The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice.
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spelling pubmed-66830362019-08-09 Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study Santiago-López, Lourdes Hernández-Mendoza, Adrián Vallejo-Cordoba, Belinda Mata-Haro, Verónica Wall-Medrano, Abraham González-Córdova, Aarón F. Nutrients Article The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice. MDPI 2019-07-16 /pmc/articles/PMC6683036/ /pubmed/31315186 http://dx.doi.org/10.3390/nu11071610 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santiago-López, Lourdes
Hernández-Mendoza, Adrián
Vallejo-Cordoba, Belinda
Mata-Haro, Verónica
Wall-Medrano, Abraham
González-Córdova, Aarón F.
Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
title Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
title_full Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
title_fullStr Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
title_full_unstemmed Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
title_short Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
title_sort milk fermented with lactobacillus fermentum ameliorates indomethacin-induced intestinal inflammation: an exploratory study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683036/
https://www.ncbi.nlm.nih.gov/pubmed/31315186
http://dx.doi.org/10.3390/nu11071610
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