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The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly

Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the effects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in different age groups. In a randomized, double-bl...

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Autores principales: Wilms, Ellen, Jonkers, Daisy M.A.E., Savelkoul, Huub F.J., Elizalde, Montserrat, Tischmann, Lea, de Vos, Paul, Masclee, Ad A.M., Troost, Freddy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683049/
https://www.ncbi.nlm.nih.gov/pubmed/31324040
http://dx.doi.org/10.3390/nu11071554
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author Wilms, Ellen
Jonkers, Daisy M.A.E.
Savelkoul, Huub F.J.
Elizalde, Montserrat
Tischmann, Lea
de Vos, Paul
Masclee, Ad A.M.
Troost, Freddy J.
author_facet Wilms, Ellen
Jonkers, Daisy M.A.E.
Savelkoul, Huub F.J.
Elizalde, Montserrat
Tischmann, Lea
de Vos, Paul
Masclee, Ad A.M.
Troost, Freddy J.
author_sort Wilms, Ellen
collection PubMed
description Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the effects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in different age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not affect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly different between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no differences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not affected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.
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spelling pubmed-66830492019-08-09 The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly Wilms, Ellen Jonkers, Daisy M.A.E. Savelkoul, Huub F.J. Elizalde, Montserrat Tischmann, Lea de Vos, Paul Masclee, Ad A.M. Troost, Freddy J. Nutrients Article Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the effects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in different age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not affect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly different between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no differences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not affected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly. MDPI 2019-07-09 /pmc/articles/PMC6683049/ /pubmed/31324040 http://dx.doi.org/10.3390/nu11071554 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wilms, Ellen
Jonkers, Daisy M.A.E.
Savelkoul, Huub F.J.
Elizalde, Montserrat
Tischmann, Lea
de Vos, Paul
Masclee, Ad A.M.
Troost, Freddy J.
The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly
title The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly
title_full The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly
title_fullStr The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly
title_full_unstemmed The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly
title_short The Impact of Pectin Supplementation on Intestinal Barrier Function in Healthy Young Adults and Healthy Elderly
title_sort impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683049/
https://www.ncbi.nlm.nih.gov/pubmed/31324040
http://dx.doi.org/10.3390/nu11071554
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