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Constraints to counting bioluminescence producing cells by a commonly used transgene promoter and its implications for experimental design
It is routine to genetically modify cells to express fluorescent or bioluminescent reporter proteins to enable tracking or quantification of cells in vitro and in vivo. Herein, we characterized the stability of luciferase reporter systems in C4-2B prostate cancer cells in mono-culture and in co-cult...
Autores principales: | Mosaad, E. O., Futrega, K., Seim, I., Gloss, B., Chambers, K. F., Clements, J. A., Doran, M. R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683182/ https://www.ncbi.nlm.nih.gov/pubmed/31383876 http://dx.doi.org/10.1038/s41598-019-46916-z |
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