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SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility

The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent...

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Autores principales: Awadia, Sahezeel, Huq, Farah, Arnold, Torey R., Goicoechea, Silvia M., Sun, Young Joo, Hou, Titus, Kreider-Letterman, Gabriel, Massimi, Paola, Banks, Lawrence, Fuentes, Ernesto J., Miller, Ann L., Garcia-Mata, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683736/
https://www.ncbi.nlm.nih.gov/pubmed/31248911
http://dx.doi.org/10.1083/jcb.201811114
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author Awadia, Sahezeel
Huq, Farah
Arnold, Torey R.
Goicoechea, Silvia M.
Sun, Young Joo
Hou, Titus
Kreider-Letterman, Gabriel
Massimi, Paola
Banks, Lawrence
Fuentes, Ernesto J.
Miller, Ann L.
Garcia-Mata, Rafael
author_facet Awadia, Sahezeel
Huq, Farah
Arnold, Torey R.
Goicoechea, Silvia M.
Sun, Young Joo
Hou, Titus
Kreider-Letterman, Gabriel
Massimi, Paola
Banks, Lawrence
Fuentes, Ernesto J.
Miller, Ann L.
Garcia-Mata, Rafael
author_sort Awadia, Sahezeel
collection PubMed
description The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent fashion and functions in the regulation of actomyosin-based contractility and barrier function at tight junctions as well as E-cadherin–mediated formation of adherens junctions. Surprisingly, SGEF does not control the establishment of polarity. However, in 3D cysts, SGEF regulates the formation of a single open lumen. Interestingly, SGEF’s nucleotide exchange activity regulates the formation and maintenance of adherens junctions, and in cysts the number of lumens formed, whereas SGEF’s scaffolding activity is critical for regulation of actomyosin contractility and lumen opening. We propose that SGEF plays a key role in coordinating junctional assembly and actomyosin contractility by bringing together Scribble and Dlg1 and targeting RhoG activation to cell–cell junctions.
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spelling pubmed-66837362020-02-05 SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility Awadia, Sahezeel Huq, Farah Arnold, Torey R. Goicoechea, Silvia M. Sun, Young Joo Hou, Titus Kreider-Letterman, Gabriel Massimi, Paola Banks, Lawrence Fuentes, Ernesto J. Miller, Ann L. Garcia-Mata, Rafael J Cell Biol Research Articles The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent fashion and functions in the regulation of actomyosin-based contractility and barrier function at tight junctions as well as E-cadherin–mediated formation of adherens junctions. Surprisingly, SGEF does not control the establishment of polarity. However, in 3D cysts, SGEF regulates the formation of a single open lumen. Interestingly, SGEF’s nucleotide exchange activity regulates the formation and maintenance of adherens junctions, and in cysts the number of lumens formed, whereas SGEF’s scaffolding activity is critical for regulation of actomyosin contractility and lumen opening. We propose that SGEF plays a key role in coordinating junctional assembly and actomyosin contractility by bringing together Scribble and Dlg1 and targeting RhoG activation to cell–cell junctions. Rockefeller University Press 2019-08-05 2019-06-27 /pmc/articles/PMC6683736/ /pubmed/31248911 http://dx.doi.org/10.1083/jcb.201811114 Text en © 2019 Awadia et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Awadia, Sahezeel
Huq, Farah
Arnold, Torey R.
Goicoechea, Silvia M.
Sun, Young Joo
Hou, Titus
Kreider-Letterman, Gabriel
Massimi, Paola
Banks, Lawrence
Fuentes, Ernesto J.
Miller, Ann L.
Garcia-Mata, Rafael
SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility
title SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility
title_full SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility
title_fullStr SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility
title_full_unstemmed SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility
title_short SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility
title_sort sgef forms a complex with scribble and dlg1 and regulates epithelial junctions and contractility
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683736/
https://www.ncbi.nlm.nih.gov/pubmed/31248911
http://dx.doi.org/10.1083/jcb.201811114
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