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Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar

Glaesserella parasuis is the cause of Glӓsser’s disease in pigs and is a significant contributor to post-weaning mortality in the swine industry. Prevention of G. parasuis disease relies primarily on bacterin vaccines, which have shown good homologous protection and variable heterologous protection....

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Autores principales: Hau, Samantha J., Mou, Kathy T., Bayles, Darrell O., Brockmeier, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684057/
https://www.ncbi.nlm.nih.gov/pubmed/31386681
http://dx.doi.org/10.1371/journal.pone.0220365
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author Hau, Samantha J.
Mou, Kathy T.
Bayles, Darrell O.
Brockmeier, Susan L.
author_facet Hau, Samantha J.
Mou, Kathy T.
Bayles, Darrell O.
Brockmeier, Susan L.
author_sort Hau, Samantha J.
collection PubMed
description Glaesserella parasuis is the cause of Glӓsser’s disease in pigs and is a significant contributor to post-weaning mortality in the swine industry. Prevention of G. parasuis disease relies primarily on bacterin vaccines, which have shown good homologous protection and variable heterologous protection. Bacterin production involves large scale growth of the bacteria and proteins produced during the proliferation phase of production become important antigens that stimulate the immune response. In order to evaluate genes activated during G. parasuis growth on different media substrates, the transcriptome of broth and agar grown G. parasuis strain 29755 were sequenced and compared. The transcription of most purported virulence genes were comparable between broth and agar grown G. parasuis; however, four virulence-associated genes, including ompA and vapD, had elevated expression under agar growth, while six virulence-associate genes had elevated expression during broth growth, including several protease genes. Additionally, there were metabolic shifts toward increased protein and lipid production and increased cellular division in broth grown G. parasuis. The results contribute to the understanding of how growth substrate alters gene transcription and protein expression, which may impact vaccine efficacy if immunogens important to the protective immune response are not produced under specific in vitro conditions. While the results of this work are unable to fully elucidate which growth medium presents a transcriptome more representative of in vivo samples or best suited for bacterin production, it forms a foundation that can be used for future comparisons and provides a better understanding of the metabolic differences in broth and agar grown bacteria.
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spelling pubmed-66840572019-08-15 Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar Hau, Samantha J. Mou, Kathy T. Bayles, Darrell O. Brockmeier, Susan L. PLoS One Research Article Glaesserella parasuis is the cause of Glӓsser’s disease in pigs and is a significant contributor to post-weaning mortality in the swine industry. Prevention of G. parasuis disease relies primarily on bacterin vaccines, which have shown good homologous protection and variable heterologous protection. Bacterin production involves large scale growth of the bacteria and proteins produced during the proliferation phase of production become important antigens that stimulate the immune response. In order to evaluate genes activated during G. parasuis growth on different media substrates, the transcriptome of broth and agar grown G. parasuis strain 29755 were sequenced and compared. The transcription of most purported virulence genes were comparable between broth and agar grown G. parasuis; however, four virulence-associated genes, including ompA and vapD, had elevated expression under agar growth, while six virulence-associate genes had elevated expression during broth growth, including several protease genes. Additionally, there were metabolic shifts toward increased protein and lipid production and increased cellular division in broth grown G. parasuis. The results contribute to the understanding of how growth substrate alters gene transcription and protein expression, which may impact vaccine efficacy if immunogens important to the protective immune response are not produced under specific in vitro conditions. While the results of this work are unable to fully elucidate which growth medium presents a transcriptome more representative of in vivo samples or best suited for bacterin production, it forms a foundation that can be used for future comparisons and provides a better understanding of the metabolic differences in broth and agar grown bacteria. Public Library of Science 2019-08-06 /pmc/articles/PMC6684057/ /pubmed/31386681 http://dx.doi.org/10.1371/journal.pone.0220365 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Hau, Samantha J.
Mou, Kathy T.
Bayles, Darrell O.
Brockmeier, Susan L.
Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar
title Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar
title_full Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar
title_fullStr Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar
title_full_unstemmed Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar
title_short Transcriptomic differences noted in Glaesserella parasuis between growth in broth and on agar
title_sort transcriptomic differences noted in glaesserella parasuis between growth in broth and on agar
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684057/
https://www.ncbi.nlm.nih.gov/pubmed/31386681
http://dx.doi.org/10.1371/journal.pone.0220365
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