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European propolis is highly active against trypanosomatids including Crithidia fasciculata

Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a paras...

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Autores principales: Alotaibi, Abdullah, Ebiloma, Godwin U., Williams, Roderick, Alenezi, Samya, Donachie, Anne-Marie, Guillaume, Selome, Igoli, John O., Fearnley, James, de Koning, Harry P., Watson, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684629/
https://www.ncbi.nlm.nih.gov/pubmed/31388043
http://dx.doi.org/10.1038/s41598-019-47840-y
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author Alotaibi, Abdullah
Ebiloma, Godwin U.
Williams, Roderick
Alenezi, Samya
Donachie, Anne-Marie
Guillaume, Selome
Igoli, John O.
Fearnley, James
de Koning, Harry P.
Watson, David G.
author_facet Alotaibi, Abdullah
Ebiloma, Godwin U.
Williams, Roderick
Alenezi, Samya
Donachie, Anne-Marie
Guillaume, Selome
Igoli, John O.
Fearnley, James
de Koning, Harry P.
Watson, David G.
author_sort Alotaibi, Abdullah
collection PubMed
description Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a parasite of bees. Crithidia, Trypanosoma and Leishmania are all members of the order Kinetoplastida. High levels of activity were obtained for all the samples with the levels of activity varying across the sample set. The highest levels of activity were found against L. mexicana. The propolis samples were profiled by using liquid chromatography with high resolution mass spectrometry (LC-MS) and principal components analysis (PCA) of the data obtained indicated there was a wide variation in the composition of the propolis samples. Orthogonal partial least squares (OPLS) associated a butyrate ester of pinobanksin with high activity against T. brucei whereas in the case of T. congolense high activity was associated with methyl ethers of chrysin and pinobanksin. In the case of C. fasciculata highest activity was associated with methyl ethers of galangin and pinobanksin. OPLS modelling of the activities against L. mexicana using the mass spectrometry produced a less successful model suggesting a wider range of active components.
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spelling pubmed-66846292019-08-11 European propolis is highly active against trypanosomatids including Crithidia fasciculata Alotaibi, Abdullah Ebiloma, Godwin U. Williams, Roderick Alenezi, Samya Donachie, Anne-Marie Guillaume, Selome Igoli, John O. Fearnley, James de Koning, Harry P. Watson, David G. Sci Rep Article Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a parasite of bees. Crithidia, Trypanosoma and Leishmania are all members of the order Kinetoplastida. High levels of activity were obtained for all the samples with the levels of activity varying across the sample set. The highest levels of activity were found against L. mexicana. The propolis samples were profiled by using liquid chromatography with high resolution mass spectrometry (LC-MS) and principal components analysis (PCA) of the data obtained indicated there was a wide variation in the composition of the propolis samples. Orthogonal partial least squares (OPLS) associated a butyrate ester of pinobanksin with high activity against T. brucei whereas in the case of T. congolense high activity was associated with methyl ethers of chrysin and pinobanksin. In the case of C. fasciculata highest activity was associated with methyl ethers of galangin and pinobanksin. OPLS modelling of the activities against L. mexicana using the mass spectrometry produced a less successful model suggesting a wider range of active components. Nature Publishing Group UK 2019-08-06 /pmc/articles/PMC6684629/ /pubmed/31388043 http://dx.doi.org/10.1038/s41598-019-47840-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Alotaibi, Abdullah
Ebiloma, Godwin U.
Williams, Roderick
Alenezi, Samya
Donachie, Anne-Marie
Guillaume, Selome
Igoli, John O.
Fearnley, James
de Koning, Harry P.
Watson, David G.
European propolis is highly active against trypanosomatids including Crithidia fasciculata
title European propolis is highly active against trypanosomatids including Crithidia fasciculata
title_full European propolis is highly active against trypanosomatids including Crithidia fasciculata
title_fullStr European propolis is highly active against trypanosomatids including Crithidia fasciculata
title_full_unstemmed European propolis is highly active against trypanosomatids including Crithidia fasciculata
title_short European propolis is highly active against trypanosomatids including Crithidia fasciculata
title_sort european propolis is highly active against trypanosomatids including crithidia fasciculata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684629/
https://www.ncbi.nlm.nih.gov/pubmed/31388043
http://dx.doi.org/10.1038/s41598-019-47840-y
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