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Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii
Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6685990/ https://www.ncbi.nlm.nih.gov/pubmed/31391460 http://dx.doi.org/10.1038/s41467-019-11376-6 |
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author | Straub, Christopher T. Khatibi, Piyum A. Wang, Jack P. Conway, Jonathan M. Williams-Rhaesa, Amanda M. Peszlen, Ilona M. Chiang, Vincent L. Adams, Michael W. W. Kelly, Robert M. |
author_facet | Straub, Christopher T. Khatibi, Piyum A. Wang, Jack P. Conway, Jonathan M. Williams-Rhaesa, Amanda M. Peszlen, Ilona M. Chiang, Vincent L. Adams, Michael W. W. Kelly, Robert M. |
author_sort | Straub, Christopher T. |
collection | PubMed |
description | Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. |
format | Online Article Text |
id | pubmed-6685990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66859902019-08-09 Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii Straub, Christopher T. Khatibi, Piyum A. Wang, Jack P. Conway, Jonathan M. Williams-Rhaesa, Amanda M. Peszlen, Ilona M. Chiang, Vincent L. Adams, Michael W. W. Kelly, Robert M. Nat Commun Article Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. Nature Publishing Group UK 2019-08-07 /pmc/articles/PMC6685990/ /pubmed/31391460 http://dx.doi.org/10.1038/s41467-019-11376-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Straub, Christopher T. Khatibi, Piyum A. Wang, Jack P. Conway, Jonathan M. Williams-Rhaesa, Amanda M. Peszlen, Ilona M. Chiang, Vincent L. Adams, Michael W. W. Kelly, Robert M. Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii |
title | Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii |
title_full | Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii |
title_fullStr | Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii |
title_full_unstemmed | Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii |
title_short | Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii |
title_sort | quantitative fermentation of unpretreated transgenic poplar by caldicellulosiruptor bescii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6685990/ https://www.ncbi.nlm.nih.gov/pubmed/31391460 http://dx.doi.org/10.1038/s41467-019-11376-6 |
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