Cargando…

Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase

There is an urgent requirement for sustainable sources of food and feed due to world population growth. Aquaculture relies heavily on the fish meal and fish oils derived from capture fisheries, challenging sustainability of the production system. Furthermore, substitution of fish oil with vegetable...

Descripción completa

Detalles Bibliográficos
Autores principales: Pudney, Alex, Gandini, Chiara, Economou, Chloe K., Smith, Richard, Goddard, Paul, Napier, Johnathan A., Spicer, Andrew, Sayanova, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686013/
https://www.ncbi.nlm.nih.gov/pubmed/31391507
http://dx.doi.org/10.1038/s41598-019-47875-1
_version_ 1783442480099753984
author Pudney, Alex
Gandini, Chiara
Economou, Chloe K.
Smith, Richard
Goddard, Paul
Napier, Johnathan A.
Spicer, Andrew
Sayanova, Olga
author_facet Pudney, Alex
Gandini, Chiara
Economou, Chloe K.
Smith, Richard
Goddard, Paul
Napier, Johnathan A.
Spicer, Andrew
Sayanova, Olga
author_sort Pudney, Alex
collection PubMed
description There is an urgent requirement for sustainable sources of food and feed due to world population growth. Aquaculture relies heavily on the fish meal and fish oils derived from capture fisheries, challenging sustainability of the production system. Furthermore, substitution of fish oil with vegetable oil and fish meal with plant seed meals in aquaculture feeds reduces the levels of valuable omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, and lowers the nutritional value due to the presence of phytate. Addition of exogenous phytase to fish feed is beneficial for enhancing animal health and reducing phosphorus pollution. We have engineered the marine diatom Phaeodactylum tricornutum, accumulating high levels of EPA and DHA together with recombinant proteins: the fungal Aspergillus niger PhyA or the bacterial Escherichia coli AppA phytases. The removal of the N-terminal signal peptide further increased phytase activity. Strains engineered with fcpA and CIP1 promoters showed the highest level of phytase activity. The best engineered strain achieved up to 40,000 phytase activity units (FTU) per gram of soluble protein, thus demonstrating the feasibility of development of multifunctionalized microalgae to simultaneously produce industrially useful proteins and fatty acids to meet the demand of intensive fish farming activity.
format Online
Article
Text
id pubmed-6686013
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66860132019-08-12 Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase Pudney, Alex Gandini, Chiara Economou, Chloe K. Smith, Richard Goddard, Paul Napier, Johnathan A. Spicer, Andrew Sayanova, Olga Sci Rep Article There is an urgent requirement for sustainable sources of food and feed due to world population growth. Aquaculture relies heavily on the fish meal and fish oils derived from capture fisheries, challenging sustainability of the production system. Furthermore, substitution of fish oil with vegetable oil and fish meal with plant seed meals in aquaculture feeds reduces the levels of valuable omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, and lowers the nutritional value due to the presence of phytate. Addition of exogenous phytase to fish feed is beneficial for enhancing animal health and reducing phosphorus pollution. We have engineered the marine diatom Phaeodactylum tricornutum, accumulating high levels of EPA and DHA together with recombinant proteins: the fungal Aspergillus niger PhyA or the bacterial Escherichia coli AppA phytases. The removal of the N-terminal signal peptide further increased phytase activity. Strains engineered with fcpA and CIP1 promoters showed the highest level of phytase activity. The best engineered strain achieved up to 40,000 phytase activity units (FTU) per gram of soluble protein, thus demonstrating the feasibility of development of multifunctionalized microalgae to simultaneously produce industrially useful proteins and fatty acids to meet the demand of intensive fish farming activity. Nature Publishing Group UK 2019-08-07 /pmc/articles/PMC6686013/ /pubmed/31391507 http://dx.doi.org/10.1038/s41598-019-47875-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pudney, Alex
Gandini, Chiara
Economou, Chloe K.
Smith, Richard
Goddard, Paul
Napier, Johnathan A.
Spicer, Andrew
Sayanova, Olga
Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
title Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
title_full Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
title_fullStr Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
title_full_unstemmed Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
title_short Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
title_sort multifunctionalizing the marine diatom phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686013/
https://www.ncbi.nlm.nih.gov/pubmed/31391507
http://dx.doi.org/10.1038/s41598-019-47875-1
work_keys_str_mv AT pudneyalex multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT gandinichiara multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT economouchloek multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT smithrichard multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT goddardpaul multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT napierjohnathana multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT spicerandrew multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase
AT sayanovaolga multifunctionalizingthemarinediatomphaeodactylumtricornutumforsustainablecoproductionofomega3longchainpolyunsaturatedfattyacidsandrecombinantphytase