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Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA

AIM: Codium fragile, an invasive seaweed, has spread widely during the last century, impacting on local seaweed communities through competition and disturbance. Early detection of C. fragile can help on its control and management. Environmental DNA (eDNA) has proved successful for early detection of...

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Autores principales: Muha, Teja Petra, Skukan, Roberta, Borrell, Yaisel J., Rico, José M., Garcia de Leaniz, Carlos, Garcia‐Vazquez, Eva, Consuegra, Sofia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686311/
https://www.ncbi.nlm.nih.gov/pubmed/31410262
http://dx.doi.org/10.1002/ece3.5379
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author Muha, Teja Petra
Skukan, Roberta
Borrell, Yaisel J.
Rico, José M.
Garcia de Leaniz, Carlos
Garcia‐Vazquez, Eva
Consuegra, Sofia
author_facet Muha, Teja Petra
Skukan, Roberta
Borrell, Yaisel J.
Rico, José M.
Garcia de Leaniz, Carlos
Garcia‐Vazquez, Eva
Consuegra, Sofia
author_sort Muha, Teja Petra
collection PubMed
description AIM: Codium fragile, an invasive seaweed, has spread widely during the last century, impacting on local seaweed communities through competition and disturbance. Early detection of C. fragile can help on its control and management. Environmental DNA (eDNA) has proved successful for early detection of aquatic invasive species but its potential use for seaweed remains understudied. We used a species‐specific eDNA qPCR approach to investigate the spatial distribution, abundance, and coexistence of the invasive C. fragile and three native Codium species (Codium vermilara, Codium tomentosum, and Codium decorticatum) in the Cantabrian Sea. LOCATION: Bay of Biscay, Northern Atlantic Coast of the Iberian Peninsula; two ports, a beach and a rocky cliff. METHODS: We designed species‐specific primers in barcoding regions targeting short fragments of the rbcL gene for the invasive Codium species, and the elongation factor Tu (tufA) gene for the native species, to assess their spatial and seasonal distributions using quantitative real‐time PCR in samples collected during summer, autumn, and winter. RESULTS: We found seasonal differences in the presence of the invasive Codium fragile and two of the native Codium species, but did not detect C. decorticatum at any point. Species distribution patterns produced with qPCR targeting species‐specific eDNA coincided with the known distribution based on previous conventional sampling, with a seasonal alternance of C. fragile and C. vermilara, and a marked dominance of invasive C. fragile in ports, which are known hotspots for invasive species. MAIN CONCLUSIONS: Our results demonstrate the utility of using eDNA for early detection and monitoring of invasive seaweed. Native and invasive Codium spp. displayed significant seasonal and spatial differentiation that needs to be taken into account in risk management. Regular monitoring of ports and adjacent areas using eDNA should help to assess the potential expansion of invasive Codium and the need for management interventions to avoid the displacement of native seaweed.
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spelling pubmed-66863112019-08-13 Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA Muha, Teja Petra Skukan, Roberta Borrell, Yaisel J. Rico, José M. Garcia de Leaniz, Carlos Garcia‐Vazquez, Eva Consuegra, Sofia Ecol Evol Original Research AIM: Codium fragile, an invasive seaweed, has spread widely during the last century, impacting on local seaweed communities through competition and disturbance. Early detection of C. fragile can help on its control and management. Environmental DNA (eDNA) has proved successful for early detection of aquatic invasive species but its potential use for seaweed remains understudied. We used a species‐specific eDNA qPCR approach to investigate the spatial distribution, abundance, and coexistence of the invasive C. fragile and three native Codium species (Codium vermilara, Codium tomentosum, and Codium decorticatum) in the Cantabrian Sea. LOCATION: Bay of Biscay, Northern Atlantic Coast of the Iberian Peninsula; two ports, a beach and a rocky cliff. METHODS: We designed species‐specific primers in barcoding regions targeting short fragments of the rbcL gene for the invasive Codium species, and the elongation factor Tu (tufA) gene for the native species, to assess their spatial and seasonal distributions using quantitative real‐time PCR in samples collected during summer, autumn, and winter. RESULTS: We found seasonal differences in the presence of the invasive Codium fragile and two of the native Codium species, but did not detect C. decorticatum at any point. Species distribution patterns produced with qPCR targeting species‐specific eDNA coincided with the known distribution based on previous conventional sampling, with a seasonal alternance of C. fragile and C. vermilara, and a marked dominance of invasive C. fragile in ports, which are known hotspots for invasive species. MAIN CONCLUSIONS: Our results demonstrate the utility of using eDNA for early detection and monitoring of invasive seaweed. Native and invasive Codium spp. displayed significant seasonal and spatial differentiation that needs to be taken into account in risk management. Regular monitoring of ports and adjacent areas using eDNA should help to assess the potential expansion of invasive Codium and the need for management interventions to avoid the displacement of native seaweed. John Wiley and Sons Inc. 2019-07-10 /pmc/articles/PMC6686311/ /pubmed/31410262 http://dx.doi.org/10.1002/ece3.5379 Text en © 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Muha, Teja Petra
Skukan, Roberta
Borrell, Yaisel J.
Rico, José M.
Garcia de Leaniz, Carlos
Garcia‐Vazquez, Eva
Consuegra, Sofia
Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA
title Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA
title_full Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA
title_fullStr Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA
title_full_unstemmed Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA
title_short Contrasting seasonal and spatial distribution of native and invasive Codium seaweed revealed by targeting species‐specific eDNA
title_sort contrasting seasonal and spatial distribution of native and invasive codium seaweed revealed by targeting species‐specific edna
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686311/
https://www.ncbi.nlm.nih.gov/pubmed/31410262
http://dx.doi.org/10.1002/ece3.5379
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