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An elusive electron shuttle from a facultative anaerobe
Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study sugge...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687433/ https://www.ncbi.nlm.nih.gov/pubmed/31232690 http://dx.doi.org/10.7554/eLife.48054 |
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author | Mevers, Emily Su, Lin Pishchany, Gleb Baruch, Moshe Cornejo, Jose Hobert, Elissa Dimise, Eric Ajo-Franklin, Caroline M Clardy, Jon |
author_facet | Mevers, Emily Su, Lin Pishchany, Gleb Baruch, Moshe Cornejo, Jose Hobert, Elissa Dimise, Eric Ajo-Franklin, Caroline M Clardy, Jon |
author_sort | Mevers, Emily |
collection | PubMed |
description | Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study suggested that Shewanella oneidensis MR-1 utilizes a small, polar, redox active molecule that serves as an electron shuttle between the bacteria and insoluble acceptors, but the shuttle itself has never been identified. Through isolation and synthesis, we identify it as ACNQ (2-amino-3-carboxy-1,4-naphthoquinone), a soluble analog of menaquinone. ACNQ is derived from DHNA (1,4-dihydroxy-2-naphthoic acid) in a non-enzymatic process that frustrated genetic approaches to identify the shuttle. Both ACNQ and DHNA restore reduction of AQDS under anaerobic growth in menaquinone-deficient mutants. Bioelectrochemistry analyses reveal that ACNQ (−0.32 V(Ag/AgCl)) contributes to the extracellular electron transfer (EET) as an electron shuttle, without altering menaquinone generation or EET related cytochrome c expression. |
format | Online Article Text |
id | pubmed-6687433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-66874332019-08-12 An elusive electron shuttle from a facultative anaerobe Mevers, Emily Su, Lin Pishchany, Gleb Baruch, Moshe Cornejo, Jose Hobert, Elissa Dimise, Eric Ajo-Franklin, Caroline M Clardy, Jon eLife Biochemistry and Chemical Biology Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study suggested that Shewanella oneidensis MR-1 utilizes a small, polar, redox active molecule that serves as an electron shuttle between the bacteria and insoluble acceptors, but the shuttle itself has never been identified. Through isolation and synthesis, we identify it as ACNQ (2-amino-3-carboxy-1,4-naphthoquinone), a soluble analog of menaquinone. ACNQ is derived from DHNA (1,4-dihydroxy-2-naphthoic acid) in a non-enzymatic process that frustrated genetic approaches to identify the shuttle. Both ACNQ and DHNA restore reduction of AQDS under anaerobic growth in menaquinone-deficient mutants. Bioelectrochemistry analyses reveal that ACNQ (−0.32 V(Ag/AgCl)) contributes to the extracellular electron transfer (EET) as an electron shuttle, without altering menaquinone generation or EET related cytochrome c expression. eLife Sciences Publications, Ltd 2019-06-24 /pmc/articles/PMC6687433/ /pubmed/31232690 http://dx.doi.org/10.7554/eLife.48054 Text en © 2019, Mevers et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Mevers, Emily Su, Lin Pishchany, Gleb Baruch, Moshe Cornejo, Jose Hobert, Elissa Dimise, Eric Ajo-Franklin, Caroline M Clardy, Jon An elusive electron shuttle from a facultative anaerobe |
title | An elusive electron shuttle from a facultative anaerobe |
title_full | An elusive electron shuttle from a facultative anaerobe |
title_fullStr | An elusive electron shuttle from a facultative anaerobe |
title_full_unstemmed | An elusive electron shuttle from a facultative anaerobe |
title_short | An elusive electron shuttle from a facultative anaerobe |
title_sort | elusive electron shuttle from a facultative anaerobe |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687433/ https://www.ncbi.nlm.nih.gov/pubmed/31232690 http://dx.doi.org/10.7554/eLife.48054 |
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