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An elusive electron shuttle from a facultative anaerobe

Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study sugge...

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Autores principales: Mevers, Emily, Su, Lin, Pishchany, Gleb, Baruch, Moshe, Cornejo, Jose, Hobert, Elissa, Dimise, Eric, Ajo-Franklin, Caroline M, Clardy, Jon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687433/
https://www.ncbi.nlm.nih.gov/pubmed/31232690
http://dx.doi.org/10.7554/eLife.48054
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author Mevers, Emily
Su, Lin
Pishchany, Gleb
Baruch, Moshe
Cornejo, Jose
Hobert, Elissa
Dimise, Eric
Ajo-Franklin, Caroline M
Clardy, Jon
author_facet Mevers, Emily
Su, Lin
Pishchany, Gleb
Baruch, Moshe
Cornejo, Jose
Hobert, Elissa
Dimise, Eric
Ajo-Franklin, Caroline M
Clardy, Jon
author_sort Mevers, Emily
collection PubMed
description Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study suggested that Shewanella oneidensis MR-1 utilizes a small, polar, redox active molecule that serves as an electron shuttle between the bacteria and insoluble acceptors, but the shuttle itself has never been identified. Through isolation and synthesis, we identify it as ACNQ (2-amino-3-carboxy-1,4-naphthoquinone), a soluble analog of menaquinone. ACNQ is derived from DHNA (1,4-dihydroxy-2-naphthoic acid) in a non-enzymatic process that frustrated genetic approaches to identify the shuttle. Both ACNQ and DHNA restore reduction of AQDS under anaerobic growth in menaquinone-deficient mutants. Bioelectrochemistry analyses reveal that ACNQ (−0.32 V(Ag/AgCl)) contributes to the extracellular electron transfer (EET) as an electron shuttle, without altering menaquinone generation or EET related cytochrome c expression.
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spelling pubmed-66874332019-08-12 An elusive electron shuttle from a facultative anaerobe Mevers, Emily Su, Lin Pishchany, Gleb Baruch, Moshe Cornejo, Jose Hobert, Elissa Dimise, Eric Ajo-Franklin, Caroline M Clardy, Jon eLife Biochemistry and Chemical Biology Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study suggested that Shewanella oneidensis MR-1 utilizes a small, polar, redox active molecule that serves as an electron shuttle between the bacteria and insoluble acceptors, but the shuttle itself has never been identified. Through isolation and synthesis, we identify it as ACNQ (2-amino-3-carboxy-1,4-naphthoquinone), a soluble analog of menaquinone. ACNQ is derived from DHNA (1,4-dihydroxy-2-naphthoic acid) in a non-enzymatic process that frustrated genetic approaches to identify the shuttle. Both ACNQ and DHNA restore reduction of AQDS under anaerobic growth in menaquinone-deficient mutants. Bioelectrochemistry analyses reveal that ACNQ (−0.32 V(Ag/AgCl)) contributes to the extracellular electron transfer (EET) as an electron shuttle, without altering menaquinone generation or EET related cytochrome c expression. eLife Sciences Publications, Ltd 2019-06-24 /pmc/articles/PMC6687433/ /pubmed/31232690 http://dx.doi.org/10.7554/eLife.48054 Text en © 2019, Mevers et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Mevers, Emily
Su, Lin
Pishchany, Gleb
Baruch, Moshe
Cornejo, Jose
Hobert, Elissa
Dimise, Eric
Ajo-Franklin, Caroline M
Clardy, Jon
An elusive electron shuttle from a facultative anaerobe
title An elusive electron shuttle from a facultative anaerobe
title_full An elusive electron shuttle from a facultative anaerobe
title_fullStr An elusive electron shuttle from a facultative anaerobe
title_full_unstemmed An elusive electron shuttle from a facultative anaerobe
title_short An elusive electron shuttle from a facultative anaerobe
title_sort elusive electron shuttle from a facultative anaerobe
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687433/
https://www.ncbi.nlm.nih.gov/pubmed/31232690
http://dx.doi.org/10.7554/eLife.48054
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