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Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions
INTRODUCTION: Zoonotic cutaneous leishmaniasis (ZCL), due to infection by Leishmania (L). major, is characterized by polymorphic clinical manifestations which could be attributed to the host's immune response. In this study we investigated the involvement of cytotoxic cells on the outcome of th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688079/ https://www.ncbi.nlm.nih.gov/pubmed/30997749 http://dx.doi.org/10.1002/iid3.240 |
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author | Boussoffara, Thouraya Boubaker, Mohamed Samir Ben Ahmed, Melika Mokni, Mourad Feriani, Salma Ben Salah, Afif Louzir, Hechmi |
author_facet | Boussoffara, Thouraya Boubaker, Mohamed Samir Ben Ahmed, Melika Mokni, Mourad Feriani, Salma Ben Salah, Afif Louzir, Hechmi |
author_sort | Boussoffara, Thouraya |
collection | PubMed |
description | INTRODUCTION: Zoonotic cutaneous leishmaniasis (ZCL), due to infection by Leishmania (L). major, is characterized by polymorphic clinical manifestations which could be attributed to the host's immune response. In this study we investigated the involvement of cytotoxic cells on the outcome of the disease. METHODS: Expression of granzyme B (GrB), granulysine (Grly), and interferon (IFN)‐γ was evaluated within ZCL lesion specimens using the technique of real‐time quantitative polymerase chain reaction (RT‐qPCR). Immunohistochemical staining was performed using anti‐CD3, CD4, CD8, CD56, GrB, and IFN‐γ antibodies to identify the phenotype of GrB and IFN‐γ‐producing cells. RESULTS: GrB and Grly mRNA was detected within 75% and 80% of ZCL lesions, respectively. Statistical analysis demonstrated a significant correlation between levels of GrB and Grly. Interestingly, expression of these molecules correlates negatively with the lesion's age. The highest levels were measured in early lesions (E‐ZCL) (lesion age ≤1 month) comparing to late lesions (L‐ZCL) (lesion age >1 month). Otherwise, IFN‐γ mRNA was detected only within 56% and a positive correlation was found between levels of this cytokine and those of GrB. Immunohistochemical analysis showed that GrB is produced essentially by CD8(+)T cells whereas IFN‐γ is produced by both CD4(+) and CD8(+)T cells. CONCLUSION: Together our results demonstrate the presence of cytotoxic cells producing GrB and Grly within leishmaniasis cutaneous lesions. |
format | Online Article Text |
id | pubmed-6688079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66880792019-08-14 Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions Boussoffara, Thouraya Boubaker, Mohamed Samir Ben Ahmed, Melika Mokni, Mourad Feriani, Salma Ben Salah, Afif Louzir, Hechmi Immun Inflamm Dis Original Research INTRODUCTION: Zoonotic cutaneous leishmaniasis (ZCL), due to infection by Leishmania (L). major, is characterized by polymorphic clinical manifestations which could be attributed to the host's immune response. In this study we investigated the involvement of cytotoxic cells on the outcome of the disease. METHODS: Expression of granzyme B (GrB), granulysine (Grly), and interferon (IFN)‐γ was evaluated within ZCL lesion specimens using the technique of real‐time quantitative polymerase chain reaction (RT‐qPCR). Immunohistochemical staining was performed using anti‐CD3, CD4, CD8, CD56, GrB, and IFN‐γ antibodies to identify the phenotype of GrB and IFN‐γ‐producing cells. RESULTS: GrB and Grly mRNA was detected within 75% and 80% of ZCL lesions, respectively. Statistical analysis demonstrated a significant correlation between levels of GrB and Grly. Interestingly, expression of these molecules correlates negatively with the lesion's age. The highest levels were measured in early lesions (E‐ZCL) (lesion age ≤1 month) comparing to late lesions (L‐ZCL) (lesion age >1 month). Otherwise, IFN‐γ mRNA was detected only within 56% and a positive correlation was found between levels of this cytokine and those of GrB. Immunohistochemical analysis showed that GrB is produced essentially by CD8(+)T cells whereas IFN‐γ is produced by both CD4(+) and CD8(+)T cells. CONCLUSION: Together our results demonstrate the presence of cytotoxic cells producing GrB and Grly within leishmaniasis cutaneous lesions. John Wiley and Sons Inc. 2019-04-17 /pmc/articles/PMC6688079/ /pubmed/30997749 http://dx.doi.org/10.1002/iid3.240 Text en © 2019 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Boussoffara, Thouraya Boubaker, Mohamed Samir Ben Ahmed, Melika Mokni, Mourad Feriani, Salma Ben Salah, Afif Louzir, Hechmi Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions |
title | Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions |
title_full | Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions |
title_fullStr | Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions |
title_full_unstemmed | Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions |
title_short | Activated cytotoxic T cells within zoonotic cutaneous leishmaniasis lesions |
title_sort | activated cytotoxic t cells within zoonotic cutaneous leishmaniasis lesions |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688079/ https://www.ncbi.nlm.nih.gov/pubmed/30997749 http://dx.doi.org/10.1002/iid3.240 |
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