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Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

Considering the significance of biological and eco-friendly nanomaterials, in the present study, we have synthesized silver nanoparticles from the exopolysaccharide of recently recovered bacterial strain CEES51 from the Red Sea coastal area of Jeddah, Saudi Arabia. 16S ribosomal RNA gene sequencing...

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Autores principales: Oves, Mohammad, Rauf, Mohd Ahmar, Hussain, Afzal, Qari, Huda A., Khan, Aftab Aslam Parwaz, Muhammad, Pir, Rehman, Md Tabish, Alajmi, Mohammad Fahad, Ismail, Iqbal I. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688106/
https://www.ncbi.nlm.nih.gov/pubmed/31427961
http://dx.doi.org/10.3389/fphar.2019.00801
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author Oves, Mohammad
Rauf, Mohd Ahmar
Hussain, Afzal
Qari, Huda A.
Khan, Aftab Aslam Parwaz
Muhammad, Pir
Rehman, Md Tabish
Alajmi, Mohammad Fahad
Ismail, Iqbal I. M.
author_facet Oves, Mohammad
Rauf, Mohd Ahmar
Hussain, Afzal
Qari, Huda A.
Khan, Aftab Aslam Parwaz
Muhammad, Pir
Rehman, Md Tabish
Alajmi, Mohammad Fahad
Ismail, Iqbal I. M.
author_sort Oves, Mohammad
collection PubMed
description Considering the significance of biological and eco-friendly nanomaterials, in the present study, we have synthesized silver nanoparticles from the exopolysaccharide of recently recovered bacterial strain CEES51 from the Red Sea coastal area of Jeddah, Saudi Arabia. 16S ribosomal RNA gene sequencing was used to characterize the isolated bacteria, and it was identified as Mesoflavibacter zeaxanthinifaciens and assigned an accession number MH707257.1 GenBank. The bacterial strain is an excellent exopolysaccharide producer and survived at hypersaline (30%) and high-temperature (50°C) conditions. The bacterial exopolysaccharides were employed for the fabrication of silver nanoparticles at room temperature. UV-visible spectrophotometer optimized the synthesized nanoparticles, and their size was determined by Nanophox particle size analyzer and dynamic light scattering. Additionally, the X-ray powder diffraction and Fourier-transform infrared spectroscopy studies also approved its crystalline nature and the involvement of organic functional groups in their formation. The synthesized nanomaterials were tested for their antibacterial and antibiofilm properties against pathogenic microorganisms Bacillus subtilis and methicillin-resistant Staphylococcus aureus. The antimicrobial property showed time, and dose-dependent response with a maximum of zone inhibition was observed at around 22 and 18 mm at a dose of 50 µg/well against B. subtilis and S. aureus and a minimum inhibitory concentration of 8 and 10 µg/ml, respectively. Furthermore, the synthesized silver nanoparticles possessed a substantial antibiofilm property and were also found to be biocompatible as depicted by red blood cell lysis assay and their interaction with peripheral blood mononuclear cells and human embryonic kidney 293 cells. Therefore, Mesoflavibacter zeaxanthinifaciens is found to be an excellent source for exopolysaccharide synthesis that assists in the silver nanoparticle production.
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spelling pubmed-66881062019-08-19 Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation Oves, Mohammad Rauf, Mohd Ahmar Hussain, Afzal Qari, Huda A. Khan, Aftab Aslam Parwaz Muhammad, Pir Rehman, Md Tabish Alajmi, Mohammad Fahad Ismail, Iqbal I. M. Front Pharmacol Pharmacology Considering the significance of biological and eco-friendly nanomaterials, in the present study, we have synthesized silver nanoparticles from the exopolysaccharide of recently recovered bacterial strain CEES51 from the Red Sea coastal area of Jeddah, Saudi Arabia. 16S ribosomal RNA gene sequencing was used to characterize the isolated bacteria, and it was identified as Mesoflavibacter zeaxanthinifaciens and assigned an accession number MH707257.1 GenBank. The bacterial strain is an excellent exopolysaccharide producer and survived at hypersaline (30%) and high-temperature (50°C) conditions. The bacterial exopolysaccharides were employed for the fabrication of silver nanoparticles at room temperature. UV-visible spectrophotometer optimized the synthesized nanoparticles, and their size was determined by Nanophox particle size analyzer and dynamic light scattering. Additionally, the X-ray powder diffraction and Fourier-transform infrared spectroscopy studies also approved its crystalline nature and the involvement of organic functional groups in their formation. The synthesized nanomaterials were tested for their antibacterial and antibiofilm properties against pathogenic microorganisms Bacillus subtilis and methicillin-resistant Staphylococcus aureus. The antimicrobial property showed time, and dose-dependent response with a maximum of zone inhibition was observed at around 22 and 18 mm at a dose of 50 µg/well against B. subtilis and S. aureus and a minimum inhibitory concentration of 8 and 10 µg/ml, respectively. Furthermore, the synthesized silver nanoparticles possessed a substantial antibiofilm property and were also found to be biocompatible as depicted by red blood cell lysis assay and their interaction with peripheral blood mononuclear cells and human embryonic kidney 293 cells. Therefore, Mesoflavibacter zeaxanthinifaciens is found to be an excellent source for exopolysaccharide synthesis that assists in the silver nanoparticle production. Frontiers Media S.A. 2019-08-02 /pmc/articles/PMC6688106/ /pubmed/31427961 http://dx.doi.org/10.3389/fphar.2019.00801 Text en Copyright © 2019 Oves, Rauf, Hussain, Qari, Khan, Muhammad, Rehman, Alajmi and Ismail http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Oves, Mohammad
Rauf, Mohd Ahmar
Hussain, Afzal
Qari, Huda A.
Khan, Aftab Aslam Parwaz
Muhammad, Pir
Rehman, Md Tabish
Alajmi, Mohammad Fahad
Ismail, Iqbal I. M.
Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation
title Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation
title_full Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation
title_fullStr Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation
title_full_unstemmed Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation
title_short Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation
title_sort antibacterial silver nanomaterial synthesis from mesoflavibacter zeaxanthinifaciens and targeting biofilm formation
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688106/
https://www.ncbi.nlm.nih.gov/pubmed/31427961
http://dx.doi.org/10.3389/fphar.2019.00801
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