Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases

BACKGROUND: The discovery of thermostable DNA polymerases such as Taq DNA polymerase revolutionized amplification of DNA by polymerase chain reaction methods that rely on thermal cycling for strand separation. These methods are widely used in the laboratory for medical research, clinical diagnostics...

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Detalles Bibliográficos
Autores principales: Piotrowski, Yvonne, Gurung, Man Kumari, Larsen, Atle Noralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688381/
https://www.ncbi.nlm.nih.gov/pubmed/31399021
http://dx.doi.org/10.1186/s12860-019-0216-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688381/
https://www.ncbi.nlm.nih.gov/pubmed/31399021
http://dx.doi.org/10.1186/s12860-019-0216-1

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