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Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid

Diet has been shown to influence epigenetic key players, such as DNA methylation, which can regulate the gene expression potential in both parents and offspring. Diets enriched in omega-6 and deficient in omega-3 PUFAs (low dietary omega-3/omega-6 PUFA ratio), have been associated with the promotion...

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Autores principales: Adam, Anne-Catrin, Lie, Kai Kristoffer, Whatmore, Paul, Jakt, Lars Martin, Moren, Mari, Skjærven, Kaja Helvik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688801/
https://www.ncbi.nlm.nih.gov/pubmed/31398226
http://dx.doi.org/10.1371/journal.pone.0220934
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author Adam, Anne-Catrin
Lie, Kai Kristoffer
Whatmore, Paul
Jakt, Lars Martin
Moren, Mari
Skjærven, Kaja Helvik
author_facet Adam, Anne-Catrin
Lie, Kai Kristoffer
Whatmore, Paul
Jakt, Lars Martin
Moren, Mari
Skjærven, Kaja Helvik
author_sort Adam, Anne-Catrin
collection PubMed
description Diet has been shown to influence epigenetic key players, such as DNA methylation, which can regulate the gene expression potential in both parents and offspring. Diets enriched in omega-6 and deficient in omega-3 PUFAs (low dietary omega-3/omega-6 PUFA ratio), have been associated with the promotion of pathogenesis of diseases in humans and other mammals. In this study, we investigated the impact of increased dietary intake of arachidonic acid (ARA), a physiologically important omega-6 PUFA, on 2 generations of zebrafish. Parental fish were fed either a low or a high ARA diet, while the progeny of both groups were fed the low ARA diet. We screened for DNA methylation on single base-pair resolution using reduced representation bisulfite sequencing (RRBS). The DNA methylation profiling revealed significant differences between the dietary groups in both parents and offspring. The majority of differentially methylated loci associated with high dietary ARA were found in introns and intergenic regions for both generations. Common loci between the identified differentially methylated loci in F(0) and F(1) livers were reported. We described overlapping gene annotations of identified methylation changes with differential expression, but based on a small number of overlaps. The present study describes the diet-associated methylation profiles across genomic regions, and it demonstrates that parental high dietary ARA modulates DNA methylation patterns in zebrafish liver.
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spelling pubmed-66888012019-08-15 Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid Adam, Anne-Catrin Lie, Kai Kristoffer Whatmore, Paul Jakt, Lars Martin Moren, Mari Skjærven, Kaja Helvik PLoS One Research Article Diet has been shown to influence epigenetic key players, such as DNA methylation, which can regulate the gene expression potential in both parents and offspring. Diets enriched in omega-6 and deficient in omega-3 PUFAs (low dietary omega-3/omega-6 PUFA ratio), have been associated with the promotion of pathogenesis of diseases in humans and other mammals. In this study, we investigated the impact of increased dietary intake of arachidonic acid (ARA), a physiologically important omega-6 PUFA, on 2 generations of zebrafish. Parental fish were fed either a low or a high ARA diet, while the progeny of both groups were fed the low ARA diet. We screened for DNA methylation on single base-pair resolution using reduced representation bisulfite sequencing (RRBS). The DNA methylation profiling revealed significant differences between the dietary groups in both parents and offspring. The majority of differentially methylated loci associated with high dietary ARA were found in introns and intergenic regions for both generations. Common loci between the identified differentially methylated loci in F(0) and F(1) livers were reported. We described overlapping gene annotations of identified methylation changes with differential expression, but based on a small number of overlaps. The present study describes the diet-associated methylation profiles across genomic regions, and it demonstrates that parental high dietary ARA modulates DNA methylation patterns in zebrafish liver. Public Library of Science 2019-08-09 /pmc/articles/PMC6688801/ /pubmed/31398226 http://dx.doi.org/10.1371/journal.pone.0220934 Text en © 2019 Adam et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Adam, Anne-Catrin
Lie, Kai Kristoffer
Whatmore, Paul
Jakt, Lars Martin
Moren, Mari
Skjærven, Kaja Helvik
Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
title Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
title_full Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
title_fullStr Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
title_full_unstemmed Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
title_short Profiling DNA methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
title_sort profiling dna methylation patterns of zebrafish liver associated with parental high dietary arachidonic acid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688801/
https://www.ncbi.nlm.nih.gov/pubmed/31398226
http://dx.doi.org/10.1371/journal.pone.0220934
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