Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and siz...

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Autores principales: Zhao, Kening, Bleackley, Mark, Chisanga, David, Gangoda, Lahiru, Fonseka, Pamali, Liem, Michael, Kalra, Hina, Al Saffar, Haidar, Keerthikumar, Shivakumar, Ang, Ching-Seng, Adda, Christopher G., Jiang, Lanzhou, Yap, Kuok, Poon, Ivan K., Lock, Peter, Bulone, Vincent, Anderson, Marilyn, Mathivanan, Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688994/
https://www.ncbi.nlm.nih.gov/pubmed/31428693
http://dx.doi.org/10.1038/s42003-019-0538-8
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author Zhao, Kening
Bleackley, Mark
Chisanga, David
Gangoda, Lahiru
Fonseka, Pamali
Liem, Michael
Kalra, Hina
Al Saffar, Haidar
Keerthikumar, Shivakumar
Ang, Ching-Seng
Adda, Christopher G.
Jiang, Lanzhou
Yap, Kuok
Poon, Ivan K.
Lock, Peter
Bulone, Vincent
Anderson, Marilyn
Mathivanan, Suresh
author_facet Zhao, Kening
Bleackley, Mark
Chisanga, David
Gangoda, Lahiru
Fonseka, Pamali
Liem, Michael
Kalra, Hina
Al Saffar, Haidar
Keerthikumar, Shivakumar
Ang, Ching-Seng
Adda, Christopher G.
Jiang, Lanzhou
Yap, Kuok
Poon, Ivan K.
Lock, Peter
Bulone, Vincent
Anderson, Marilyn
Mathivanan, Suresh
author_sort Zhao, Kening
collection PubMed
description Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.
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spelling pubmed-66889942019-08-19 Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling Zhao, Kening Bleackley, Mark Chisanga, David Gangoda, Lahiru Fonseka, Pamali Liem, Michael Kalra, Hina Al Saffar, Haidar Keerthikumar, Shivakumar Ang, Ching-Seng Adda, Christopher G. Jiang, Lanzhou Yap, Kuok Poon, Ivan K. Lock, Peter Bulone, Vincent Anderson, Marilyn Mathivanan, Suresh Commun Biol Article Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling. Nature Publishing Group UK 2019-08-09 /pmc/articles/PMC6688994/ /pubmed/31428693 http://dx.doi.org/10.1038/s42003-019-0538-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhao, Kening
Bleackley, Mark
Chisanga, David
Gangoda, Lahiru
Fonseka, Pamali
Liem, Michael
Kalra, Hina
Al Saffar, Haidar
Keerthikumar, Shivakumar
Ang, Ching-Seng
Adda, Christopher G.
Jiang, Lanzhou
Yap, Kuok
Poon, Ivan K.
Lock, Peter
Bulone, Vincent
Anderson, Marilyn
Mathivanan, Suresh
Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
title Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
title_full Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
title_fullStr Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
title_full_unstemmed Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
title_short Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
title_sort extracellular vesicles secreted by saccharomyces cerevisiae are involved in cell wall remodelling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688994/
https://www.ncbi.nlm.nih.gov/pubmed/31428693
http://dx.doi.org/10.1038/s42003-019-0538-8
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