Cargando…

Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics

PURPOSE: We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study...

Descripción completa

Detalles Bibliográficos
Autores principales: Sandberg, Jonna, Kovatcheva-Datchary, Petia, Björck, Inger, Bäckhed, Fredrik, Nilsson, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689319/
https://www.ncbi.nlm.nih.gov/pubmed/30046942
http://dx.doi.org/10.1007/s00394-018-1788-9
_version_ 1783443018041262080
author Sandberg, Jonna
Kovatcheva-Datchary, Petia
Björck, Inger
Bäckhed, Fredrik
Nilsson, Anne
author_facet Sandberg, Jonna
Kovatcheva-Datchary, Petia
Björck, Inger
Bäckhed, Fredrik
Nilsson, Anne
author_sort Sandberg, Jonna
collection PubMed
description PURPOSE: We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study was to investigate if the baseline Prevotella/Bacteroides ratio can be used to stratify metabolic responders and non-responders to barley dietary fiber (DF). METHODS: Fecal samples were collected from 99 healthy humans with BMI < 28 kg/m(2) between 50 and 70 years old. The abundance of fecal Prevotella and Bacteroides was quantified with 16S rRNA quantitative PCR. 33 subjects were grouped in three groups: subjects with highest Prevotella/Bacteroides ratios, “HP”, n = 12; subjects with lowest Prevotella/Bacteroides ratios, “LP”, n = 13; and subjects with high abundance of both measured bacteria, HPB, n = 8. A 3-day randomized crossover intervention with BKB and white wheat bread (control) was performed. Cardiometabolic test variables were analyzed the next day following a standardized breakfast. RESULTS: The BKB intervention lowered the blood glucose responses to the breakfast independently of Prevotella/Bacteroides ratios (P < 0.01). However, independently of intervention, the HP group displayed an overall lower insulin response and lower IL-6 concentrations compared with the LP group (P < 0.05). Furthermore, the groups HP and HPB showed lower hunger sensations compared to the LP group (P < 0.05). CONCLUSIONS: Here we show that the abundance of gut Prevotella and Bacteroides at baseline did not stratify metabolic responders and non-responders to barley DF intervention. However, our results indicate the importance of gut microbiota in host metabolic regulation, further suggesting that higher Prevotella/Bacteroides ratio may be favorable. CLINICALTRIALS.GOV ID: NCT02427555 ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00394-018-1788-9) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6689319
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-66893192019-08-23 Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics Sandberg, Jonna Kovatcheva-Datchary, Petia Björck, Inger Bäckhed, Fredrik Nilsson, Anne Eur J Nutr Original Contribution PURPOSE: We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study was to investigate if the baseline Prevotella/Bacteroides ratio can be used to stratify metabolic responders and non-responders to barley dietary fiber (DF). METHODS: Fecal samples were collected from 99 healthy humans with BMI < 28 kg/m(2) between 50 and 70 years old. The abundance of fecal Prevotella and Bacteroides was quantified with 16S rRNA quantitative PCR. 33 subjects were grouped in three groups: subjects with highest Prevotella/Bacteroides ratios, “HP”, n = 12; subjects with lowest Prevotella/Bacteroides ratios, “LP”, n = 13; and subjects with high abundance of both measured bacteria, HPB, n = 8. A 3-day randomized crossover intervention with BKB and white wheat bread (control) was performed. Cardiometabolic test variables were analyzed the next day following a standardized breakfast. RESULTS: The BKB intervention lowered the blood glucose responses to the breakfast independently of Prevotella/Bacteroides ratios (P < 0.01). However, independently of intervention, the HP group displayed an overall lower insulin response and lower IL-6 concentrations compared with the LP group (P < 0.05). Furthermore, the groups HP and HPB showed lower hunger sensations compared to the LP group (P < 0.05). CONCLUSIONS: Here we show that the abundance of gut Prevotella and Bacteroides at baseline did not stratify metabolic responders and non-responders to barley DF intervention. However, our results indicate the importance of gut microbiota in host metabolic regulation, further suggesting that higher Prevotella/Bacteroides ratio may be favorable. CLINICALTRIALS.GOV ID: NCT02427555 ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00394-018-1788-9) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-07-25 2019 /pmc/articles/PMC6689319/ /pubmed/30046942 http://dx.doi.org/10.1007/s00394-018-1788-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Contribution
Sandberg, Jonna
Kovatcheva-Datchary, Petia
Björck, Inger
Bäckhed, Fredrik
Nilsson, Anne
Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
title Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
title_full Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
title_fullStr Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
title_full_unstemmed Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
title_short Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
title_sort abundance of gut prevotella at baseline and metabolic response to barley prebiotics
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689319/
https://www.ncbi.nlm.nih.gov/pubmed/30046942
http://dx.doi.org/10.1007/s00394-018-1788-9
work_keys_str_mv AT sandbergjonna abundanceofgutprevotellaatbaselineandmetabolicresponsetobarleyprebiotics
AT kovatchevadatcharypetia abundanceofgutprevotellaatbaselineandmetabolicresponsetobarleyprebiotics
AT bjorckinger abundanceofgutprevotellaatbaselineandmetabolicresponsetobarleyprebiotics
AT backhedfredrik abundanceofgutprevotellaatbaselineandmetabolicresponsetobarleyprebiotics
AT nilssonanne abundanceofgutprevotellaatbaselineandmetabolicresponsetobarleyprebiotics