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Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy

Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when...

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Detalles Bibliográficos
Autores principales: Johnstone, Graeme E., Cairns, Gemma S., Patton, Brian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689623/
https://www.ncbi.nlm.nih.gov/pubmed/31417755
http://dx.doi.org/10.1098/rsos.190589
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author Johnstone, Graeme E.
Cairns, Gemma S.
Patton, Brian R.
author_facet Johnstone, Graeme E.
Cairns, Gemma S.
Patton, Brian R.
author_sort Johnstone, Graeme E.
collection PubMed
description Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.
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spelling pubmed-66896232019-08-15 Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy Johnstone, Graeme E. Cairns, Gemma S. Patton, Brian R. R Soc Open Sci Physics Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms. The Royal Society 2019-07-31 /pmc/articles/PMC6689623/ /pubmed/31417755 http://dx.doi.org/10.1098/rsos.190589 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Physics
Johnstone, Graeme E.
Cairns, Gemma S.
Patton, Brian R.
Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
title Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
title_full Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
title_fullStr Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
title_full_unstemmed Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
title_short Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
title_sort nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
topic Physics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689623/
https://www.ncbi.nlm.nih.gov/pubmed/31417755
http://dx.doi.org/10.1098/rsos.190589
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