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Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model

INTRODUCTION: Type 2 diabetes (T2D) is a widely distributed disease that affects large population worldwide. This study aimed to verify the role of Ginkgo biloba (GB) extract and magnetized water (MW) on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rats. MAT...

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Autores principales: Saleh, Ahmed, Anwar, Mamdouh M, Zayed, Ahmed E, Ezz Eldeen, Manal El Sayed, Afifi, Gamal, Alnashiri, Hassien M, Gomaa, Asmaa MS, Abd-Elkareem, Mahmoud, Abou-Elhamd, Alaa Sayed, Shaheen, Emad S, Mohamed, Ghada A, Hetta, Helal F, Kotb, Ahmed M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689767/
https://www.ncbi.nlm.nih.gov/pubmed/31496771
http://dx.doi.org/10.2147/DMSO.S209856
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author Saleh, Ahmed
Anwar, Mamdouh M
Zayed, Ahmed E
Ezz Eldeen, Manal El Sayed
Afifi, Gamal
Alnashiri, Hassien M
Gomaa, Asmaa MS
Abd-Elkareem, Mahmoud
Abou-Elhamd, Alaa Sayed
Shaheen, Emad S
Mohamed, Ghada A
Hetta, Helal F
Kotb, Ahmed M
author_facet Saleh, Ahmed
Anwar, Mamdouh M
Zayed, Ahmed E
Ezz Eldeen, Manal El Sayed
Afifi, Gamal
Alnashiri, Hassien M
Gomaa, Asmaa MS
Abd-Elkareem, Mahmoud
Abou-Elhamd, Alaa Sayed
Shaheen, Emad S
Mohamed, Ghada A
Hetta, Helal F
Kotb, Ahmed M
author_sort Saleh, Ahmed
collection PubMed
description INTRODUCTION: Type 2 diabetes (T2D) is a widely distributed disease that affects large population worldwide. This study aimed to verify the role of Ginkgo biloba (GB) extract and magnetized water (MW) on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rats. MATERIALS AND METHODS: T2D was induced by feeding the rats on a high-fat diet (20% fat, 45% carbohydrate, 22% protein) for eight weeks followed by intra-peritoneal injection of a single low dose of streptozotocin (25mg/Kg). Forty rats were randomly assigned to four groups (n=10 rats) as follows: non treated control and three diabetic groups. One diabetic group served as a positive control (diabetic), while the other two groups were orally administered with water extract of GB leaves (0.11 g/kg/day) and MW (600 gauss) for four weeks, respectively. RESULTS: The β-cell mass and insulin expression in these cells increased markedly after both treatments, particularly in GB treated group. In addition, the immune-expression of the two antioxidant enzymes; glutathione and superoxide dismutase 2 (SOD2) in the pancreatic tissue demonstrated a down-regulation in GB and MW treated groups as compared with the diabetic group. CONCLUSION: A four-week treatment of GB and MW protected pancreatic β-cell cells and improved their insulin expression and antioxidant status in type 2 diabetic rats.
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spelling pubmed-66897672019-09-06 Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model Saleh, Ahmed Anwar, Mamdouh M Zayed, Ahmed E Ezz Eldeen, Manal El Sayed Afifi, Gamal Alnashiri, Hassien M Gomaa, Asmaa MS Abd-Elkareem, Mahmoud Abou-Elhamd, Alaa Sayed Shaheen, Emad S Mohamed, Ghada A Hetta, Helal F Kotb, Ahmed M Diabetes Metab Syndr Obes Original Research INTRODUCTION: Type 2 diabetes (T2D) is a widely distributed disease that affects large population worldwide. This study aimed to verify the role of Ginkgo biloba (GB) extract and magnetized water (MW) on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rats. MATERIALS AND METHODS: T2D was induced by feeding the rats on a high-fat diet (20% fat, 45% carbohydrate, 22% protein) for eight weeks followed by intra-peritoneal injection of a single low dose of streptozotocin (25mg/Kg). Forty rats were randomly assigned to four groups (n=10 rats) as follows: non treated control and three diabetic groups. One diabetic group served as a positive control (diabetic), while the other two groups were orally administered with water extract of GB leaves (0.11 g/kg/day) and MW (600 gauss) for four weeks, respectively. RESULTS: The β-cell mass and insulin expression in these cells increased markedly after both treatments, particularly in GB treated group. In addition, the immune-expression of the two antioxidant enzymes; glutathione and superoxide dismutase 2 (SOD2) in the pancreatic tissue demonstrated a down-regulation in GB and MW treated groups as compared with the diabetic group. CONCLUSION: A four-week treatment of GB and MW protected pancreatic β-cell cells and improved their insulin expression and antioxidant status in type 2 diabetic rats. Dove 2019-08-07 /pmc/articles/PMC6689767/ /pubmed/31496771 http://dx.doi.org/10.2147/DMSO.S209856 Text en © 2019 Saleh et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Saleh, Ahmed
Anwar, Mamdouh M
Zayed, Ahmed E
Ezz Eldeen, Manal El Sayed
Afifi, Gamal
Alnashiri, Hassien M
Gomaa, Asmaa MS
Abd-Elkareem, Mahmoud
Abou-Elhamd, Alaa Sayed
Shaheen, Emad S
Mohamed, Ghada A
Hetta, Helal F
Kotb, Ahmed M
Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
title Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
title_full Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
title_fullStr Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
title_full_unstemmed Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
title_short Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
title_sort impact of ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689767/
https://www.ncbi.nlm.nih.gov/pubmed/31496771
http://dx.doi.org/10.2147/DMSO.S209856
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