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MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition

The NuRD complex contains both chromatin remodeling and histone deacetylase activities. Mice lacking the MTA2 subunit of NuRD show developmental defects in pro-B, pre-B, immature B, and marginal zone B cells, and abnormal germinal center B cell differentiation during immune responses. Mta2 inactivat...

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Autores principales: Lu, Xiangdong, Chu, Chi-Shuen, Fang, Terry, Rayon-Estrada, Violeta, Fang, Fang, Patke, Alina, Qian, Ye, Clarke, Stephen H., Melnick, Ari M., Zhang, Yi, Papavasiliou, F. Nina, Roeder, Robert G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690613/
https://www.ncbi.nlm.nih.gov/pubmed/31291582
http://dx.doi.org/10.1016/j.celrep.2019.06.029
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author Lu, Xiangdong
Chu, Chi-Shuen
Fang, Terry
Rayon-Estrada, Violeta
Fang, Fang
Patke, Alina
Qian, Ye
Clarke, Stephen H.
Melnick, Ari M.
Zhang, Yi
Papavasiliou, F. Nina
Roeder, Robert G.
author_facet Lu, Xiangdong
Chu, Chi-Shuen
Fang, Terry
Rayon-Estrada, Violeta
Fang, Fang
Patke, Alina
Qian, Ye
Clarke, Stephen H.
Melnick, Ari M.
Zhang, Yi
Papavasiliou, F. Nina
Roeder, Robert G.
author_sort Lu, Xiangdong
collection PubMed
description The NuRD complex contains both chromatin remodeling and histone deacetylase activities. Mice lacking the MTA2 subunit of NuRD show developmental defects in pro-B, pre-B, immature B, and marginal zone B cells, and abnormal germinal center B cell differentiation during immune responses. Mta2 inactivation also causes a derepression of Igll1 and VpreB1 genes in pre-B cells. Furthermore, MTA2/NuRD interacts directly with AIOLOS/IKAROS and shows a striking overlap with AIOLOS/IKAROS target genes in human pre-B cells, suggesting a functional interdependence between MTA2/NuRD and AIOLOS. Mechanistically, MTA2 deficiency in mice leads to increased H3K27 acetylation at both Igll1 and VpreB1 promoters. Gene profiling analyses also identify distinct MTA2-dependent transcription programs in pro-B and pre-B cells. In addition, we find a strong synergy between MTA2 and OCA-B in repressing Igll1 and VpreB1 at the pre-B cell stage, and in regulating both the pre-B to immature B transition and splenic B cell development.
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spelling pubmed-66906132019-08-12 MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition Lu, Xiangdong Chu, Chi-Shuen Fang, Terry Rayon-Estrada, Violeta Fang, Fang Patke, Alina Qian, Ye Clarke, Stephen H. Melnick, Ari M. Zhang, Yi Papavasiliou, F. Nina Roeder, Robert G. Cell Rep Article The NuRD complex contains both chromatin remodeling and histone deacetylase activities. Mice lacking the MTA2 subunit of NuRD show developmental defects in pro-B, pre-B, immature B, and marginal zone B cells, and abnormal germinal center B cell differentiation during immune responses. Mta2 inactivation also causes a derepression of Igll1 and VpreB1 genes in pre-B cells. Furthermore, MTA2/NuRD interacts directly with AIOLOS/IKAROS and shows a striking overlap with AIOLOS/IKAROS target genes in human pre-B cells, suggesting a functional interdependence between MTA2/NuRD and AIOLOS. Mechanistically, MTA2 deficiency in mice leads to increased H3K27 acetylation at both Igll1 and VpreB1 promoters. Gene profiling analyses also identify distinct MTA2-dependent transcription programs in pro-B and pre-B cells. In addition, we find a strong synergy between MTA2 and OCA-B in repressing Igll1 and VpreB1 at the pre-B cell stage, and in regulating both the pre-B to immature B transition and splenic B cell development. 2019-07-09 /pmc/articles/PMC6690613/ /pubmed/31291582 http://dx.doi.org/10.1016/j.celrep.2019.06.029 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lu, Xiangdong
Chu, Chi-Shuen
Fang, Terry
Rayon-Estrada, Violeta
Fang, Fang
Patke, Alina
Qian, Ye
Clarke, Stephen H.
Melnick, Ari M.
Zhang, Yi
Papavasiliou, F. Nina
Roeder, Robert G.
MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition
title MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition
title_full MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition
title_fullStr MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition
title_full_unstemmed MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition
title_short MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition
title_sort mta2/nurd regulates b cell development and cooperates with oca-b in controlling the pre-b to immature b cell transition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690613/
https://www.ncbi.nlm.nih.gov/pubmed/31291582
http://dx.doi.org/10.1016/j.celrep.2019.06.029
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