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Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS

In this dataset we provide MALDI-TOF/MS spectra for the testing and application of a quantitative method using external ionization standards (ionization STDs) for peak-intensity normalization. The presented data is related to our recent article entitled “a comparative evaluation of the extraction an...

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Autores principales: Li, Xin, Nakayama, Kenji, Goto, Takayuki, Akamatsu, Shusuke, Shimizu, Koji, Ogawa, Osamu, Inoue, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690661/
https://www.ncbi.nlm.nih.gov/pubmed/31417948
http://dx.doi.org/10.1016/j.dib.2019.104275
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author Li, Xin
Nakayama, Kenji
Goto, Takayuki
Akamatsu, Shusuke
Shimizu, Koji
Ogawa, Osamu
Inoue, Takahiro
author_facet Li, Xin
Nakayama, Kenji
Goto, Takayuki
Akamatsu, Shusuke
Shimizu, Koji
Ogawa, Osamu
Inoue, Takahiro
author_sort Li, Xin
collection PubMed
description In this dataset we provide MALDI-TOF/MS spectra for the testing and application of a quantitative method using external ionization standards (ionization STDs) for peak-intensity normalization. The presented data is related to our recent article entitled “a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS”. Gradient dilutions of mixture containing thirteen phospho- and lysophospho-lipid species (internal STDs) were mixed with constant concentration of the ionization STDs and analyzed together. Peak intensities of the internal and ionization STDs were picked by a homemade workflow based on OpenMS (steps including noise filtration, baseline subtraction and peak-picking). The peak-intensity ratios between the internal and ionization STDs were linearly correlated with their concentration ratios. Using this method, the evaluation of efficiencies of six different lipid extraction methods was performed in urine samples. In summary, a free and easy-to-use method for phospholipid and lysophospholipid quantitative analysis based on MALDI-TOF/MS is provided in this article.
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spelling pubmed-66906612019-08-15 Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS Li, Xin Nakayama, Kenji Goto, Takayuki Akamatsu, Shusuke Shimizu, Koji Ogawa, Osamu Inoue, Takahiro Data Brief Biochemistry, Genetics and Molecular Biology In this dataset we provide MALDI-TOF/MS spectra for the testing and application of a quantitative method using external ionization standards (ionization STDs) for peak-intensity normalization. The presented data is related to our recent article entitled “a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS”. Gradient dilutions of mixture containing thirteen phospho- and lysophospho-lipid species (internal STDs) were mixed with constant concentration of the ionization STDs and analyzed together. Peak intensities of the internal and ionization STDs were picked by a homemade workflow based on OpenMS (steps including noise filtration, baseline subtraction and peak-picking). The peak-intensity ratios between the internal and ionization STDs were linearly correlated with their concentration ratios. Using this method, the evaluation of efficiencies of six different lipid extraction methods was performed in urine samples. In summary, a free and easy-to-use method for phospholipid and lysophospholipid quantitative analysis based on MALDI-TOF/MS is provided in this article. Elsevier 2019-07-15 /pmc/articles/PMC6690661/ /pubmed/31417948 http://dx.doi.org/10.1016/j.dib.2019.104275 Text en © 2019 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Li, Xin
Nakayama, Kenji
Goto, Takayuki
Akamatsu, Shusuke
Shimizu, Koji
Ogawa, Osamu
Inoue, Takahiro
Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS
title Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS
title_full Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS
title_fullStr Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS
title_full_unstemmed Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS
title_short Data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using MALDI-TOF/MS
title_sort data processing on a comparative evaluation of the extraction and analysis procedures for urinary phospholipid and lysophospholipid using maldi-tof/ms
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690661/
https://www.ncbi.nlm.nih.gov/pubmed/31417948
http://dx.doi.org/10.1016/j.dib.2019.104275
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