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Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera
Umami taste perception indicates the presence of amino acids, which are essential nutrients. Although the physiology of umami perception has been described in mammals, how insects detect amino acids remains unknown except in Drosophila melanogaster. We functionally characterized a gustatory receptor...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690930/ https://www.ncbi.nlm.nih.gov/pubmed/31406120 http://dx.doi.org/10.1038/s41598-019-46738-z |
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author | Lim, Sooho Jung, Jewon Yunusbaev, Ural Ilyasov, Rustem Kwon, Hyung Wook |
author_facet | Lim, Sooho Jung, Jewon Yunusbaev, Ural Ilyasov, Rustem Kwon, Hyung Wook |
author_sort | Lim, Sooho |
collection | PubMed |
description | Umami taste perception indicates the presence of amino acids, which are essential nutrients. Although the physiology of umami perception has been described in mammals, how insects detect amino acids remains unknown except in Drosophila melanogaster. We functionally characterized a gustatory receptor responding to L-amino acids in the western honey bee, Apis mellifera. Using a calcium-imaging assay and two-voltage clamp recording, we found that one of the honey bee’s gustatory receptors, AmGr10, functions as a broadly tuned amino acid receptor responding to glutamate, aspartate, asparagine, arginine, lysine, and glutamine, but not to other sweet or bitter compounds. Furthermore, the sensitivity of AmGr10 to these L-amino acids was dramatically enhanced by purine ribonucleotides, like inosine-5′-monophosphate (IMP). Contact sensory hairs in the mouthpart of the honey bee responded strongly to glutamate and aspartate, which house gustatory receptor neurons expressing AmGr10. Interestingly, AmGr10 protein is highly conserved among hymenopterans but not other insects, implying unique functions in eusocial insects. |
format | Online Article Text |
id | pubmed-6690930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66909302019-08-15 Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera Lim, Sooho Jung, Jewon Yunusbaev, Ural Ilyasov, Rustem Kwon, Hyung Wook Sci Rep Article Umami taste perception indicates the presence of amino acids, which are essential nutrients. Although the physiology of umami perception has been described in mammals, how insects detect amino acids remains unknown except in Drosophila melanogaster. We functionally characterized a gustatory receptor responding to L-amino acids in the western honey bee, Apis mellifera. Using a calcium-imaging assay and two-voltage clamp recording, we found that one of the honey bee’s gustatory receptors, AmGr10, functions as a broadly tuned amino acid receptor responding to glutamate, aspartate, asparagine, arginine, lysine, and glutamine, but not to other sweet or bitter compounds. Furthermore, the sensitivity of AmGr10 to these L-amino acids was dramatically enhanced by purine ribonucleotides, like inosine-5′-monophosphate (IMP). Contact sensory hairs in the mouthpart of the honey bee responded strongly to glutamate and aspartate, which house gustatory receptor neurons expressing AmGr10. Interestingly, AmGr10 protein is highly conserved among hymenopterans but not other insects, implying unique functions in eusocial insects. Nature Publishing Group UK 2019-08-12 /pmc/articles/PMC6690930/ /pubmed/31406120 http://dx.doi.org/10.1038/s41598-019-46738-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lim, Sooho Jung, Jewon Yunusbaev, Ural Ilyasov, Rustem Kwon, Hyung Wook Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera |
title | Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera |
title_full | Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera |
title_fullStr | Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera |
title_full_unstemmed | Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera |
title_short | Characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, Apis mellifera |
title_sort | characterization and its implication of a novel taste receptor detecting nutrients in the honey bee, apis mellifera |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690930/ https://www.ncbi.nlm.nih.gov/pubmed/31406120 http://dx.doi.org/10.1038/s41598-019-46738-z |
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