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Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this impor...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690977/ https://www.ncbi.nlm.nih.gov/pubmed/31406175 http://dx.doi.org/10.1038/s41467-019-11332-4 |
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author | Chua, Adeline C. Y. Ong, Jessica Jie Ying Malleret, Benoit Suwanarusk, Rossarin Kosaisavee, Varakorn Zeeman, Anne-Marie Cooper, Caitlin A. Tan, Kevin S. W. Zhang, Rou Tan, Bee Huat Abas, Siti Nurdiana Yip, Andy Elliot, Anne Joyner, Chester J. Cho, Jee Sun Breyer, Kate Baran, Szczepan Lange, Amber Maher, Steven P. Nosten, François Bodenreider, Christophe Yeung, Bryan K. S. Mazier, Dominique Galinski, Mary R. Dereuddre-Bosquet, Nathalie Le Grand, Roger Kocken, Clemens H. M. Rénia, Laurent Kyle, Dennis E. Diagana, Thierry T. Snounou, Georges Russell, Bruce Bifani, Pablo |
author_facet | Chua, Adeline C. Y. Ong, Jessica Jie Ying Malleret, Benoit Suwanarusk, Rossarin Kosaisavee, Varakorn Zeeman, Anne-Marie Cooper, Caitlin A. Tan, Kevin S. W. Zhang, Rou Tan, Bee Huat Abas, Siti Nurdiana Yip, Andy Elliot, Anne Joyner, Chester J. Cho, Jee Sun Breyer, Kate Baran, Szczepan Lange, Amber Maher, Steven P. Nosten, François Bodenreider, Christophe Yeung, Bryan K. S. Mazier, Dominique Galinski, Mary R. Dereuddre-Bosquet, Nathalie Le Grand, Roger Kocken, Clemens H. M. Rénia, Laurent Kyle, Dennis E. Diagana, Thierry T. Snounou, Georges Russell, Bruce Bifani, Pablo |
author_sort | Chua, Adeline C. Y. |
collection | PubMed |
description | The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts. |
format | Online Article Text |
id | pubmed-6690977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66909772019-08-14 Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages Chua, Adeline C. Y. Ong, Jessica Jie Ying Malleret, Benoit Suwanarusk, Rossarin Kosaisavee, Varakorn Zeeman, Anne-Marie Cooper, Caitlin A. Tan, Kevin S. W. Zhang, Rou Tan, Bee Huat Abas, Siti Nurdiana Yip, Andy Elliot, Anne Joyner, Chester J. Cho, Jee Sun Breyer, Kate Baran, Szczepan Lange, Amber Maher, Steven P. Nosten, François Bodenreider, Christophe Yeung, Bryan K. S. Mazier, Dominique Galinski, Mary R. Dereuddre-Bosquet, Nathalie Le Grand, Roger Kocken, Clemens H. M. Rénia, Laurent Kyle, Dennis E. Diagana, Thierry T. Snounou, Georges Russell, Bruce Bifani, Pablo Nat Commun Article The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts. Nature Publishing Group UK 2019-08-12 /pmc/articles/PMC6690977/ /pubmed/31406175 http://dx.doi.org/10.1038/s41467-019-11332-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chua, Adeline C. Y. Ong, Jessica Jie Ying Malleret, Benoit Suwanarusk, Rossarin Kosaisavee, Varakorn Zeeman, Anne-Marie Cooper, Caitlin A. Tan, Kevin S. W. Zhang, Rou Tan, Bee Huat Abas, Siti Nurdiana Yip, Andy Elliot, Anne Joyner, Chester J. Cho, Jee Sun Breyer, Kate Baran, Szczepan Lange, Amber Maher, Steven P. Nosten, François Bodenreider, Christophe Yeung, Bryan K. S. Mazier, Dominique Galinski, Mary R. Dereuddre-Bosquet, Nathalie Le Grand, Roger Kocken, Clemens H. M. Rénia, Laurent Kyle, Dennis E. Diagana, Thierry T. Snounou, Georges Russell, Bruce Bifani, Pablo Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages |
title | Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages |
title_full | Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages |
title_fullStr | Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages |
title_full_unstemmed | Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages |
title_short | Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages |
title_sort | robust continuous in vitro culture of the plasmodium cynomolgi erythrocytic stages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690977/ https://www.ncbi.nlm.nih.gov/pubmed/31406175 http://dx.doi.org/10.1038/s41467-019-11332-4 |
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