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Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages

The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this impor...

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Autores principales: Chua, Adeline C. Y., Ong, Jessica Jie Ying, Malleret, Benoit, Suwanarusk, Rossarin, Kosaisavee, Varakorn, Zeeman, Anne-Marie, Cooper, Caitlin A., Tan, Kevin S. W., Zhang, Rou, Tan, Bee Huat, Abas, Siti Nurdiana, Yip, Andy, Elliot, Anne, Joyner, Chester J., Cho, Jee Sun, Breyer, Kate, Baran, Szczepan, Lange, Amber, Maher, Steven P., Nosten, François, Bodenreider, Christophe, Yeung, Bryan K. S., Mazier, Dominique, Galinski, Mary R., Dereuddre-Bosquet, Nathalie, Le Grand, Roger, Kocken, Clemens H. M., Rénia, Laurent, Kyle, Dennis E., Diagana, Thierry T., Snounou, Georges, Russell, Bruce, Bifani, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690977/
https://www.ncbi.nlm.nih.gov/pubmed/31406175
http://dx.doi.org/10.1038/s41467-019-11332-4
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author Chua, Adeline C. Y.
Ong, Jessica Jie Ying
Malleret, Benoit
Suwanarusk, Rossarin
Kosaisavee, Varakorn
Zeeman, Anne-Marie
Cooper, Caitlin A.
Tan, Kevin S. W.
Zhang, Rou
Tan, Bee Huat
Abas, Siti Nurdiana
Yip, Andy
Elliot, Anne
Joyner, Chester J.
Cho, Jee Sun
Breyer, Kate
Baran, Szczepan
Lange, Amber
Maher, Steven P.
Nosten, François
Bodenreider, Christophe
Yeung, Bryan K. S.
Mazier, Dominique
Galinski, Mary R.
Dereuddre-Bosquet, Nathalie
Le Grand, Roger
Kocken, Clemens H. M.
Rénia, Laurent
Kyle, Dennis E.
Diagana, Thierry T.
Snounou, Georges
Russell, Bruce
Bifani, Pablo
author_facet Chua, Adeline C. Y.
Ong, Jessica Jie Ying
Malleret, Benoit
Suwanarusk, Rossarin
Kosaisavee, Varakorn
Zeeman, Anne-Marie
Cooper, Caitlin A.
Tan, Kevin S. W.
Zhang, Rou
Tan, Bee Huat
Abas, Siti Nurdiana
Yip, Andy
Elliot, Anne
Joyner, Chester J.
Cho, Jee Sun
Breyer, Kate
Baran, Szczepan
Lange, Amber
Maher, Steven P.
Nosten, François
Bodenreider, Christophe
Yeung, Bryan K. S.
Mazier, Dominique
Galinski, Mary R.
Dereuddre-Bosquet, Nathalie
Le Grand, Roger
Kocken, Clemens H. M.
Rénia, Laurent
Kyle, Dennis E.
Diagana, Thierry T.
Snounou, Georges
Russell, Bruce
Bifani, Pablo
author_sort Chua, Adeline C. Y.
collection PubMed
description The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts.
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spelling pubmed-66909772019-08-14 Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages Chua, Adeline C. Y. Ong, Jessica Jie Ying Malleret, Benoit Suwanarusk, Rossarin Kosaisavee, Varakorn Zeeman, Anne-Marie Cooper, Caitlin A. Tan, Kevin S. W. Zhang, Rou Tan, Bee Huat Abas, Siti Nurdiana Yip, Andy Elliot, Anne Joyner, Chester J. Cho, Jee Sun Breyer, Kate Baran, Szczepan Lange, Amber Maher, Steven P. Nosten, François Bodenreider, Christophe Yeung, Bryan K. S. Mazier, Dominique Galinski, Mary R. Dereuddre-Bosquet, Nathalie Le Grand, Roger Kocken, Clemens H. M. Rénia, Laurent Kyle, Dennis E. Diagana, Thierry T. Snounou, Georges Russell, Bruce Bifani, Pablo Nat Commun Article The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts. Nature Publishing Group UK 2019-08-12 /pmc/articles/PMC6690977/ /pubmed/31406175 http://dx.doi.org/10.1038/s41467-019-11332-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chua, Adeline C. Y.
Ong, Jessica Jie Ying
Malleret, Benoit
Suwanarusk, Rossarin
Kosaisavee, Varakorn
Zeeman, Anne-Marie
Cooper, Caitlin A.
Tan, Kevin S. W.
Zhang, Rou
Tan, Bee Huat
Abas, Siti Nurdiana
Yip, Andy
Elliot, Anne
Joyner, Chester J.
Cho, Jee Sun
Breyer, Kate
Baran, Szczepan
Lange, Amber
Maher, Steven P.
Nosten, François
Bodenreider, Christophe
Yeung, Bryan K. S.
Mazier, Dominique
Galinski, Mary R.
Dereuddre-Bosquet, Nathalie
Le Grand, Roger
Kocken, Clemens H. M.
Rénia, Laurent
Kyle, Dennis E.
Diagana, Thierry T.
Snounou, Georges
Russell, Bruce
Bifani, Pablo
Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
title Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
title_full Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
title_fullStr Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
title_full_unstemmed Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
title_short Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
title_sort robust continuous in vitro culture of the plasmodium cynomolgi erythrocytic stages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690977/
https://www.ncbi.nlm.nih.gov/pubmed/31406175
http://dx.doi.org/10.1038/s41467-019-11332-4
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