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MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene
The aim of the present study was to investigate the effect of microRNA (miR)-539 on the proliferation and migration of gastric cancer cells, and explore the underlying mechanism. Gastric cancer cell lines with high or low miR-539 and SRY-box 5 (SOX5) expression levels were constructed by transfectio...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691193/ https://www.ncbi.nlm.nih.gov/pubmed/31322222 http://dx.doi.org/10.3892/mmr.2019.10486 |
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author | Ding, Shi Zhang, Yanping |
author_facet | Ding, Shi Zhang, Yanping |
author_sort | Ding, Shi |
collection | PubMed |
description | The aim of the present study was to investigate the effect of microRNA (miR)-539 on the proliferation and migration of gastric cancer cells, and explore the underlying mechanism. Gastric cancer cell lines with high or low miR-539 and SRY-box 5 (SOX5) expression levels were constructed by transfection. The proliferation of gastric cancer cells was then detected by Cell Counting Kit-8 assay and cell migration was tested by transwell assay. The results revealed low expression of miR-539 and high expression of SOX5 in gastric cancer tissues and cells as compared with the levels in normal tissues and cells, suggesting that there was a negative correlation between miR-539 and SOX5. Dual-luciferase reporter experiments demonstrated that miR-539 directly targeted SOX5. The proliferation and migration of gastric cancer cells were negatively regulated by the overexpression of miR-539, while positively regulated by the overexpression of SOX5. Notably, SOX5 overexpression attenuated the inhibitory effect of miR-539 on gastric cancer cells. The results suggested that SOX5 is a target gene of miR-539, and that miR-539 inhibits the proliferation and migration of gastric cancer cells by targeting SOX5. |
format | Online Article Text |
id | pubmed-6691193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66911932019-08-19 MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene Ding, Shi Zhang, Yanping Mol Med Rep Articles The aim of the present study was to investigate the effect of microRNA (miR)-539 on the proliferation and migration of gastric cancer cells, and explore the underlying mechanism. Gastric cancer cell lines with high or low miR-539 and SRY-box 5 (SOX5) expression levels were constructed by transfection. The proliferation of gastric cancer cells was then detected by Cell Counting Kit-8 assay and cell migration was tested by transwell assay. The results revealed low expression of miR-539 and high expression of SOX5 in gastric cancer tissues and cells as compared with the levels in normal tissues and cells, suggesting that there was a negative correlation between miR-539 and SOX5. Dual-luciferase reporter experiments demonstrated that miR-539 directly targeted SOX5. The proliferation and migration of gastric cancer cells were negatively regulated by the overexpression of miR-539, while positively regulated by the overexpression of SOX5. Notably, SOX5 overexpression attenuated the inhibitory effect of miR-539 on gastric cancer cells. The results suggested that SOX5 is a target gene of miR-539, and that miR-539 inhibits the proliferation and migration of gastric cancer cells by targeting SOX5. D.A. Spandidos 2019-09 2019-07-11 /pmc/articles/PMC6691193/ /pubmed/31322222 http://dx.doi.org/10.3892/mmr.2019.10486 Text en Copyright: © Ding et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Ding, Shi Zhang, Yanping MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene |
title | MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene |
title_full | MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene |
title_fullStr | MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene |
title_full_unstemmed | MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene |
title_short | MicroRNA-539 inhibits the proliferation and migration of gastric cancer cells by targeting SRY-box 5 gene |
title_sort | microrna-539 inhibits the proliferation and migration of gastric cancer cells by targeting sry-box 5 gene |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691193/ https://www.ncbi.nlm.nih.gov/pubmed/31322222 http://dx.doi.org/10.3892/mmr.2019.10486 |
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