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Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma

OBJECTIVES: Infiltration of macrophages through the tyrosine kinase receptor CSF1R is a poor prognosis factor in various solid tumors. Indeed, these tumors produce CSF1R ligand, macrophage colony‐stimulating factor (M‐CSF) or interleukin‐34 (IL‐34). However, the significance of these cytokines, part...

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Autores principales: Noyori, Osamu, Komohara, Yoshihiro, Nasser, Hesham, Hiyoshi, Masateru, Ma, Chaoya, Pan, Cheng, Carreras, Joaquim, Nakamura, Naoya, Sato, Ai, Ando, Kiyoshi, Okuno, Yutaka, Nosaka, Kisato, Matsuoka, Masao, Suzu, Shinya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691654/
https://www.ncbi.nlm.nih.gov/pubmed/31417675
http://dx.doi.org/10.1002/cti2.1074
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author Noyori, Osamu
Komohara, Yoshihiro
Nasser, Hesham
Hiyoshi, Masateru
Ma, Chaoya
Pan, Cheng
Carreras, Joaquim
Nakamura, Naoya
Sato, Ai
Ando, Kiyoshi
Okuno, Yutaka
Nosaka, Kisato
Matsuoka, Masao
Suzu, Shinya
author_facet Noyori, Osamu
Komohara, Yoshihiro
Nasser, Hesham
Hiyoshi, Masateru
Ma, Chaoya
Pan, Cheng
Carreras, Joaquim
Nakamura, Naoya
Sato, Ai
Ando, Kiyoshi
Okuno, Yutaka
Nosaka, Kisato
Matsuoka, Masao
Suzu, Shinya
author_sort Noyori, Osamu
collection PubMed
description OBJECTIVES: Infiltration of macrophages through the tyrosine kinase receptor CSF1R is a poor prognosis factor in various solid tumors. Indeed, these tumors produce CSF1R ligand, macrophage colony‐stimulating factor (M‐CSF) or interleukin‐34 (IL‐34). However, the significance of these cytokines, particularly, the newly discovered IL‐34 in haematological malignancies, is not fully understood. We therefore analysed the role of IL‐34 in diffuse large B‐cell lymphoma (DLBCL), the most common subtype of malignant lymphoma. METHODS: We analysed formalin‐fixed paraffin‐embedded lymphoma tissues of 135 DLBCL patients for the expression of IL‐34 and the number of macrophages, and the survival of these patients. The expression of IL‐34 in DLBCL cell lines and the activity of IL‐34 to induce the migration of monocytic cells were also characterised. RESULTS: Several lymphoma tissues showed a clear IL‐34 signal, and such signal was detectable in 36% of patients. DLBCL cell lines also expressed IL‐34. Interestingly, the percentage of IL‐34(+) patients in the activated B‐cell subtype was significantly higher than that in the germinal centre B‐cell subtype. More interestingly, IL‐34(+) patients showed shorter survival periods and higher number of macrophages in lymphoma tissues. The recruitment of monocytes is likely the first step for the higher macrophage density in the IL‐34(+) lymphoma tissues. Indeed, IL‐34 induced the migration of monocytic cells. CONCLUSION: Our results raise the possibility that IL‐34 in lymphoma tissues of DLBCL patients recruits monocytes, leading to the higher number of macrophages in the tissues and poor prognosis of patients. IL‐34 may be an additional therapeutic target of DLBCL.
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spelling pubmed-66916542019-08-15 Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma Noyori, Osamu Komohara, Yoshihiro Nasser, Hesham Hiyoshi, Masateru Ma, Chaoya Pan, Cheng Carreras, Joaquim Nakamura, Naoya Sato, Ai Ando, Kiyoshi Okuno, Yutaka Nosaka, Kisato Matsuoka, Masao Suzu, Shinya Clin Transl Immunology Original Articles OBJECTIVES: Infiltration of macrophages through the tyrosine kinase receptor CSF1R is a poor prognosis factor in various solid tumors. Indeed, these tumors produce CSF1R ligand, macrophage colony‐stimulating factor (M‐CSF) or interleukin‐34 (IL‐34). However, the significance of these cytokines, particularly, the newly discovered IL‐34 in haematological malignancies, is not fully understood. We therefore analysed the role of IL‐34 in diffuse large B‐cell lymphoma (DLBCL), the most common subtype of malignant lymphoma. METHODS: We analysed formalin‐fixed paraffin‐embedded lymphoma tissues of 135 DLBCL patients for the expression of IL‐34 and the number of macrophages, and the survival of these patients. The expression of IL‐34 in DLBCL cell lines and the activity of IL‐34 to induce the migration of monocytic cells were also characterised. RESULTS: Several lymphoma tissues showed a clear IL‐34 signal, and such signal was detectable in 36% of patients. DLBCL cell lines also expressed IL‐34. Interestingly, the percentage of IL‐34(+) patients in the activated B‐cell subtype was significantly higher than that in the germinal centre B‐cell subtype. More interestingly, IL‐34(+) patients showed shorter survival periods and higher number of macrophages in lymphoma tissues. The recruitment of monocytes is likely the first step for the higher macrophage density in the IL‐34(+) lymphoma tissues. Indeed, IL‐34 induced the migration of monocytic cells. CONCLUSION: Our results raise the possibility that IL‐34 in lymphoma tissues of DLBCL patients recruits monocytes, leading to the higher number of macrophages in the tissues and poor prognosis of patients. IL‐34 may be an additional therapeutic target of DLBCL. John Wiley and Sons Inc. 2019-08-13 /pmc/articles/PMC6691654/ /pubmed/31417675 http://dx.doi.org/10.1002/cti2.1074 Text en © 2019 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Noyori, Osamu
Komohara, Yoshihiro
Nasser, Hesham
Hiyoshi, Masateru
Ma, Chaoya
Pan, Cheng
Carreras, Joaquim
Nakamura, Naoya
Sato, Ai
Ando, Kiyoshi
Okuno, Yutaka
Nosaka, Kisato
Matsuoka, Masao
Suzu, Shinya
Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma
title Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma
title_full Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma
title_fullStr Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma
title_full_unstemmed Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma
title_short Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma
title_sort expression of il‐34 correlates with macrophage infiltration and prognosis of diffuse large b‐cell lymphoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691654/
https://www.ncbi.nlm.nih.gov/pubmed/31417675
http://dx.doi.org/10.1002/cti2.1074
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