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High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer

Nuclear receptor binding protein 1 (NRBP1) is an evolutionarily highly conserved adaptor protein with multiple domains. Recently, its role in cancers has received increasing attention. To investigate whether NRBP1 is involved in the development of bladder cancer, we used tissue microarray and analyz...

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Autores principales: Wu, Qi, Zhou, Xiaoqing, Li, Peng, Wang, Wei, Wang, Jun, Tan, Mingyue, Tao, Le, Qiu, Jianxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691704/
https://www.ncbi.nlm.nih.gov/pubmed/31413746
http://dx.doi.org/10.7150/jca.32656
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author Wu, Qi
Zhou, Xiaoqing
Li, Peng
Wang, Wei
Wang, Jun
Tan, Mingyue
Tao, Le
Qiu, Jianxin
author_facet Wu, Qi
Zhou, Xiaoqing
Li, Peng
Wang, Wei
Wang, Jun
Tan, Mingyue
Tao, Le
Qiu, Jianxin
author_sort Wu, Qi
collection PubMed
description Nuclear receptor binding protein 1 (NRBP1) is an evolutionarily highly conserved adaptor protein with multiple domains. Recently, its role in cancers has received increasing attention. To investigate whether NRBP1 is involved in the development of bladder cancer, we used tissue microarray and analyzed the association between the expression levels of NRBP1 and clinico-pathological features of 56 patients diagnosed with bladder cancer. Subsequently, NRBP1 was silenced using siRNA in bladder cancer cell lines T24 and 5637, and cell phenotype such as proliferation and apoptosis were observed. Further, in vivo tumor formation assay was performed. The expression of apoptosis markers was detected by Western blot. A significant positive correlation between increased NRBP1 expression and tumor stage, and lymph node metastasis was observed in 56 patients. High expression of NRBP1 was associated with poor prognosis and NRBP1 knockdown significantly inhibited cell proliferation and induced intrinsic apoptosis in vitro. Moreover, we also found that NRBP1 knockdown significantly suppress tumor growth in xenograft mouse model. Taken together, these data suggest that NRBP1 plays a critical role in the development of bladder cancer and may represent a potential target for bladder cancer treatment.
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spelling pubmed-66917042019-08-14 High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer Wu, Qi Zhou, Xiaoqing Li, Peng Wang, Wei Wang, Jun Tan, Mingyue Tao, Le Qiu, Jianxin J Cancer Research Paper Nuclear receptor binding protein 1 (NRBP1) is an evolutionarily highly conserved adaptor protein with multiple domains. Recently, its role in cancers has received increasing attention. To investigate whether NRBP1 is involved in the development of bladder cancer, we used tissue microarray and analyzed the association between the expression levels of NRBP1 and clinico-pathological features of 56 patients diagnosed with bladder cancer. Subsequently, NRBP1 was silenced using siRNA in bladder cancer cell lines T24 and 5637, and cell phenotype such as proliferation and apoptosis were observed. Further, in vivo tumor formation assay was performed. The expression of apoptosis markers was detected by Western blot. A significant positive correlation between increased NRBP1 expression and tumor stage, and lymph node metastasis was observed in 56 patients. High expression of NRBP1 was associated with poor prognosis and NRBP1 knockdown significantly inhibited cell proliferation and induced intrinsic apoptosis in vitro. Moreover, we also found that NRBP1 knockdown significantly suppress tumor growth in xenograft mouse model. Taken together, these data suggest that NRBP1 plays a critical role in the development of bladder cancer and may represent a potential target for bladder cancer treatment. Ivyspring International Publisher 2019-07-10 /pmc/articles/PMC6691704/ /pubmed/31413746 http://dx.doi.org/10.7150/jca.32656 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Wu, Qi
Zhou, Xiaoqing
Li, Peng
Wang, Wei
Wang, Jun
Tan, Mingyue
Tao, Le
Qiu, Jianxin
High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
title High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
title_full High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
title_fullStr High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
title_full_unstemmed High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
title_short High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
title_sort high nrbp1 expression promotes proliferation and correlates with poor prognosis in bladder cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691704/
https://www.ncbi.nlm.nih.gov/pubmed/31413746
http://dx.doi.org/10.7150/jca.32656
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