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Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome

The use of assisted reproductive technologies (ART) can induce a congenital overgrowth condition in humans and ruminants, namely Beckwith-Wiedemann syndrome (BWS) and large offspring syndrome (LOS), respectively. Shared phenotypes and epigenotypes have been found between BWS and LOS. We have observe...

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Autores principales: Li, Yahan, Hagen, Darren Erich, Ji, Tieming, Bakhtiarizadeh, Mohammad Reza, Frederic, Whitney M., Traxler, Emily M., Kalish, Jennifer M., Rivera, Rocío Melissa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691986/
https://www.ncbi.nlm.nih.gov/pubmed/31144574
http://dx.doi.org/10.1080/15592294.2019.1615357
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author Li, Yahan
Hagen, Darren Erich
Ji, Tieming
Bakhtiarizadeh, Mohammad Reza
Frederic, Whitney M.
Traxler, Emily M.
Kalish, Jennifer M.
Rivera, Rocío Melissa
author_facet Li, Yahan
Hagen, Darren Erich
Ji, Tieming
Bakhtiarizadeh, Mohammad Reza
Frederic, Whitney M.
Traxler, Emily M.
Kalish, Jennifer M.
Rivera, Rocío Melissa
author_sort Li, Yahan
collection PubMed
description The use of assisted reproductive technologies (ART) can induce a congenital overgrowth condition in humans and ruminants, namely Beckwith-Wiedemann syndrome (BWS) and large offspring syndrome (LOS), respectively. Shared phenotypes and epigenotypes have been found between BWS and LOS. We have observed global misregulation of transcripts in bovine foetuses with LOS. microRNAs (miRNAs) are important post-transcriptional gene expression regulators. We hypothesize that there is miRNA misregulation in LOS and that this misregulation is shared with BWS. In this study, small RNA sequencing was conducted to investigate miRNA expression profiles in bovine and human samples. We detected 407 abundant known miRNAs and predicted 196 putative miRNAs from the bovine sequencing results and identified 505 abundant miRNAs in human tongue. Differentially expressed miRNAs (DE-miRNAs) were identified between control and LOS groups in all tissues analysed as well as between BWS and control human samples. DE-miRNAs were detected from several miRNA clusters including DLK1-DIO3 genomic imprinted cluster in LOS and BWS. DNA hypermethylation was associated with downregulation of miRNAs in the DLK1-DIO3. mRNA targets of the DE-miRNAs were predicted and signalling pathways associated with control of organ size (including the Hippo signalling pathway), cell proliferation, apoptosis, cell survival, cell cycle, and cell adhesion were found to be enriched with these genes. Yes associated protein 1 (YAP1) is the core effector of the Hippo signalling pathway, and increased level of active (non-phosphorylated) YAP1 protein was detected in skeletal muscle of LOS foetuses. Overall, our data provide evidence of miRNA misregulation in LOS and BWS.
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spelling pubmed-66919862019-08-23 Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome Li, Yahan Hagen, Darren Erich Ji, Tieming Bakhtiarizadeh, Mohammad Reza Frederic, Whitney M. Traxler, Emily M. Kalish, Jennifer M. Rivera, Rocío Melissa Epigenetics Research Paper The use of assisted reproductive technologies (ART) can induce a congenital overgrowth condition in humans and ruminants, namely Beckwith-Wiedemann syndrome (BWS) and large offspring syndrome (LOS), respectively. Shared phenotypes and epigenotypes have been found between BWS and LOS. We have observed global misregulation of transcripts in bovine foetuses with LOS. microRNAs (miRNAs) are important post-transcriptional gene expression regulators. We hypothesize that there is miRNA misregulation in LOS and that this misregulation is shared with BWS. In this study, small RNA sequencing was conducted to investigate miRNA expression profiles in bovine and human samples. We detected 407 abundant known miRNAs and predicted 196 putative miRNAs from the bovine sequencing results and identified 505 abundant miRNAs in human tongue. Differentially expressed miRNAs (DE-miRNAs) were identified between control and LOS groups in all tissues analysed as well as between BWS and control human samples. DE-miRNAs were detected from several miRNA clusters including DLK1-DIO3 genomic imprinted cluster in LOS and BWS. DNA hypermethylation was associated with downregulation of miRNAs in the DLK1-DIO3. mRNA targets of the DE-miRNAs were predicted and signalling pathways associated with control of organ size (including the Hippo signalling pathway), cell proliferation, apoptosis, cell survival, cell cycle, and cell adhesion were found to be enriched with these genes. Yes associated protein 1 (YAP1) is the core effector of the Hippo signalling pathway, and increased level of active (non-phosphorylated) YAP1 protein was detected in skeletal muscle of LOS foetuses. Overall, our data provide evidence of miRNA misregulation in LOS and BWS. Taylor & Francis 2019-05-30 /pmc/articles/PMC6691986/ /pubmed/31144574 http://dx.doi.org/10.1080/15592294.2019.1615357 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Research Paper
Li, Yahan
Hagen, Darren Erich
Ji, Tieming
Bakhtiarizadeh, Mohammad Reza
Frederic, Whitney M.
Traxler, Emily M.
Kalish, Jennifer M.
Rivera, Rocío Melissa
Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome
title Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome
title_full Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome
title_fullStr Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome
title_full_unstemmed Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome
title_short Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome
title_sort altered microrna expression profiles in large offspring syndrome and beckwith-wiedemann syndrome
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691986/
https://www.ncbi.nlm.nih.gov/pubmed/31144574
http://dx.doi.org/10.1080/15592294.2019.1615357
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