Cargando…
Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study
The 2013–2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Eb...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692041/ https://www.ncbi.nlm.nih.gov/pubmed/31369554 http://dx.doi.org/10.1371/journal.pntd.0007654 |
_version_ | 1783443496269512704 |
---|---|
author | Halfmann, Peter J. Eisfeld, Amie J. Watanabe, Tokiko Maemura, Tadashi Yamashita, Makoto Fukuyama, Satoshi Armbrust, Tammy Rozich, Isaiah N’jai, Alhaji Neumann, Gabriele Kawaoka, Yoshihiro Sahr, Foday |
author_facet | Halfmann, Peter J. Eisfeld, Amie J. Watanabe, Tokiko Maemura, Tadashi Yamashita, Makoto Fukuyama, Satoshi Armbrust, Tammy Rozich, Isaiah N’jai, Alhaji Neumann, Gabriele Kawaoka, Yoshihiro Sahr, Foday |
author_sort | Halfmann, Peter J. |
collection | PubMed |
description | The 2013–2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Ebola virus disease. In March 2017, we collected blood samples from 481 individuals in Makeni, Sierra Leone: 214 survivors and 267 close contacts. Using commercial, quantitative ELISAs, we tested the plasma for IgG-specific antibodies against three major viral antigens: GP, the only viral glycoprotein expressed on the virus surface; NP, the most abundant viral protein; and VP40, a major structural protein of Zaire ebolavirus. We also determined neutralizing antibody titers. In the cohort of Ebola survivors, 97.7% of samples (209/214) had measurable antibody levels against GP, NP, and/or VP40. Of these positive samples, all but one had measurable neutralizing antibody titers against Ebola virus. For the close contacts, up to 12.7% (34/267) may have experienced a subclinical virus infection as indicated by detectable antibodies against GP. Further investigation is warranted to determine whether these close contacts truly experienced subclinical infections and whether these asymptomatic infections played a role in the dynamics of transmission. |
format | Online Article Text |
id | pubmed-6692041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66920412019-08-30 Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study Halfmann, Peter J. Eisfeld, Amie J. Watanabe, Tokiko Maemura, Tadashi Yamashita, Makoto Fukuyama, Satoshi Armbrust, Tammy Rozich, Isaiah N’jai, Alhaji Neumann, Gabriele Kawaoka, Yoshihiro Sahr, Foday PLoS Negl Trop Dis Research Article The 2013–2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Ebola virus disease. In March 2017, we collected blood samples from 481 individuals in Makeni, Sierra Leone: 214 survivors and 267 close contacts. Using commercial, quantitative ELISAs, we tested the plasma for IgG-specific antibodies against three major viral antigens: GP, the only viral glycoprotein expressed on the virus surface; NP, the most abundant viral protein; and VP40, a major structural protein of Zaire ebolavirus. We also determined neutralizing antibody titers. In the cohort of Ebola survivors, 97.7% of samples (209/214) had measurable antibody levels against GP, NP, and/or VP40. Of these positive samples, all but one had measurable neutralizing antibody titers against Ebola virus. For the close contacts, up to 12.7% (34/267) may have experienced a subclinical virus infection as indicated by detectable antibodies against GP. Further investigation is warranted to determine whether these close contacts truly experienced subclinical infections and whether these asymptomatic infections played a role in the dynamics of transmission. Public Library of Science 2019-08-01 /pmc/articles/PMC6692041/ /pubmed/31369554 http://dx.doi.org/10.1371/journal.pntd.0007654 Text en © 2019 Halfmann et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Halfmann, Peter J. Eisfeld, Amie J. Watanabe, Tokiko Maemura, Tadashi Yamashita, Makoto Fukuyama, Satoshi Armbrust, Tammy Rozich, Isaiah N’jai, Alhaji Neumann, Gabriele Kawaoka, Yoshihiro Sahr, Foday Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study |
title | Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study |
title_full | Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study |
title_fullStr | Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study |
title_full_unstemmed | Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study |
title_short | Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study |
title_sort | serological analysis of ebola virus survivors and close contacts in sierra leone: a cross-sectional study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692041/ https://www.ncbi.nlm.nih.gov/pubmed/31369554 http://dx.doi.org/10.1371/journal.pntd.0007654 |
work_keys_str_mv | AT halfmannpeterj serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT eisfeldamiej serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT watanabetokiko serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT maemuratadashi serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT yamashitamakoto serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT fukuyamasatoshi serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT armbrusttammy serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT rozichisaiah serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT njaialhaji serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT neumanngabriele serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT kawaokayoshihiro serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy AT sahrfoday serologicalanalysisofebolavirussurvivorsandclosecontactsinsierraleoneacrosssectionalstudy |