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Phase-sensitive interferometry of decorrelated speckle patterns

Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index decorrelate the phases and speckle patterns in the images. It is...

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Autores principales: Spahr, Hendrik, Pfäffle, Clara, Burhan, Sazan, Kutzner, Lisa, Hilge, Felix, Hüttmann, Gereon, Hillmann, Dierck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692410/
https://www.ncbi.nlm.nih.gov/pubmed/31409819
http://dx.doi.org/10.1038/s41598-019-47979-8
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author Spahr, Hendrik
Pfäffle, Clara
Burhan, Sazan
Kutzner, Lisa
Hilge, Felix
Hüttmann, Gereon
Hillmann, Dierck
author_facet Spahr, Hendrik
Pfäffle, Clara
Burhan, Sazan
Kutzner, Lisa
Hilge, Felix
Hüttmann, Gereon
Hillmann, Dierck
author_sort Spahr, Hendrik
collection PubMed
description Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index decorrelate the phases and speckle patterns in the images. It is generally believed that once the speckle pattern has changed, the phases are scrambled and any meaningful phase difference to the original pattern is removed. As a consequence, diffusion and tissue motion that cannot be resolved, prevent phase-sensitive imaging of biological specimens. Here, we show that a phase comparison between decorrelated speckle patterns is still possible by utilizing a series of images acquired during decorrelation. The resulting evaluation scheme is mathematically equivalent to methods for astronomic imaging through the turbulent sky by speckle interferometry. We thus adopt the idea of speckle interferometry to phase-sensitive imaging in biological tissues and demonstrate its efficacy for simulated data and imaging of photoreceptor activity with phase-sensitive optical coherence tomography. We believe the described methods can be applied to many imaging modalities that use phase values for interferometry.
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spelling pubmed-66924102019-08-19 Phase-sensitive interferometry of decorrelated speckle patterns Spahr, Hendrik Pfäffle, Clara Burhan, Sazan Kutzner, Lisa Hilge, Felix Hüttmann, Gereon Hillmann, Dierck Sci Rep Article Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index decorrelate the phases and speckle patterns in the images. It is generally believed that once the speckle pattern has changed, the phases are scrambled and any meaningful phase difference to the original pattern is removed. As a consequence, diffusion and tissue motion that cannot be resolved, prevent phase-sensitive imaging of biological specimens. Here, we show that a phase comparison between decorrelated speckle patterns is still possible by utilizing a series of images acquired during decorrelation. The resulting evaluation scheme is mathematically equivalent to methods for astronomic imaging through the turbulent sky by speckle interferometry. We thus adopt the idea of speckle interferometry to phase-sensitive imaging in biological tissues and demonstrate its efficacy for simulated data and imaging of photoreceptor activity with phase-sensitive optical coherence tomography. We believe the described methods can be applied to many imaging modalities that use phase values for interferometry. Nature Publishing Group UK 2019-08-13 /pmc/articles/PMC6692410/ /pubmed/31409819 http://dx.doi.org/10.1038/s41598-019-47979-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Spahr, Hendrik
Pfäffle, Clara
Burhan, Sazan
Kutzner, Lisa
Hilge, Felix
Hüttmann, Gereon
Hillmann, Dierck
Phase-sensitive interferometry of decorrelated speckle patterns
title Phase-sensitive interferometry of decorrelated speckle patterns
title_full Phase-sensitive interferometry of decorrelated speckle patterns
title_fullStr Phase-sensitive interferometry of decorrelated speckle patterns
title_full_unstemmed Phase-sensitive interferometry of decorrelated speckle patterns
title_short Phase-sensitive interferometry of decorrelated speckle patterns
title_sort phase-sensitive interferometry of decorrelated speckle patterns
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692410/
https://www.ncbi.nlm.nih.gov/pubmed/31409819
http://dx.doi.org/10.1038/s41598-019-47979-8
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