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Engineering of a chromogenic enzyme screening system based on an auxiliary indole‐3‐carboxylic acid monooxygenase

Here, we present a proof‐of‐principle for a new high‐throughput functional screening of metagenomic libraries for the selection of enzymes with different activities, predetermined by the substrate being used. By this approach, a total of 21 enzyme‐coding genes were selected, including members of xan...

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Detalles Bibliográficos
Autores principales: Časaitė, Vida, Sadauskas, Mikas, Vaitekūnas, Justas, Gasparavičiūtė, Renata, Meškienė, Rita, Skikaitė, Izabelė, Sakalauskas, Mantas, Jakubovska, Jevgenija, Tauraitė, Daiva, Meškys, Rolandas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692525/
https://www.ncbi.nlm.nih.gov/pubmed/30666828
http://dx.doi.org/10.1002/mbo3.795