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High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines

This study provides a deep modern insight into the phylogenetic diversity among bacterial consortia found in working and nonworking high‐methane natural gas pipelines located in Poland. The working pipeline was characterized by lower biodiversity (140–154 bacterial genera from 22 to 23 classes, depe...

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Autores principales: Staniszewska, Agnieszka, Kunicka‐Styczyńska, Alina, Otlewska, Anna, Gawor, Jan, Gromadka, Robert, Żuchniewicz, Karolina, Ziemiński, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692550/
https://www.ncbi.nlm.nih.gov/pubmed/30729757
http://dx.doi.org/10.1002/mbo3.806
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author Staniszewska, Agnieszka
Kunicka‐Styczyńska, Alina
Otlewska, Anna
Gawor, Jan
Gromadka, Robert
Żuchniewicz, Karolina
Ziemiński, Krzysztof
author_facet Staniszewska, Agnieszka
Kunicka‐Styczyńska, Alina
Otlewska, Anna
Gawor, Jan
Gromadka, Robert
Żuchniewicz, Karolina
Ziemiński, Krzysztof
author_sort Staniszewska, Agnieszka
collection PubMed
description This study provides a deep modern insight into the phylogenetic diversity among bacterial consortia found in working and nonworking high‐methane natural gas pipelines located in Poland. The working pipeline was characterized by lower biodiversity (140–154 bacterial genera from 22 to 23 classes, depending on the source of the debris) in comparison to the off‐gas pipeline (169 bacterial genera from 23 classes). The sediment recovered from the working pipeline contained mostly DNA identified as belonging to the phylum Firmicutes (66.4%–45.9% operational taxonomic units [OTUs]), predominantly Bacillus (41.4%–31.1% OTUs) followed by Lysinibacillus (2.6%–1.5% OTUs) and Clostridium (2.4%–1.8% OTUs). In the nonworking pipeline, Proteobacteria (46.8% OTUs) and Cyanobacteria (27.8% OTUs) were dominant. Over 30% of the Proteobacteria sequences showed homologies to Gammaproteobacteria, with Pseudomonas (7.1%), Enhydrobacter (2.1%), Stenotrophomonas (0.5%), and Haempohilus (0.4%) among the others. Differences were noted in terms of the chemical compositions of deposits originating from the working and nonworking gas pipelines. The deposits from the nonworking gas pipeline contained iron, as well as carbon (42.58%), sulphur (15.27%), and oxygen (15.32%). This composition can be linked to both the quantity and type of the resident microorganisms. The presence of a considerable amount of silicon (17.42%), and of aluminum, potassium, calcium, and magnesium at detectable levels, may likewise affect the metabolic activity of the resident consortia in the working gas pipeline. All the analyzed sediments included both bacteria known for causing and intensifying corrosion (e.g., Pseudomonas, Desulfovibrio, Shewanella, Serratia) and bacteria that can protect the surface of pipelines against deterioration (e.g., Bacillus). Biocorrosion is not related to a single mechanism or one species of microorganism, but results from the multidirectional activity of multiple microbial communities. The analysis presented here of the state of the microbiome in a gas pipeline during the real gas transport is a particularly valuable element of this work.
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spelling pubmed-66925502019-08-16 High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines Staniszewska, Agnieszka Kunicka‐Styczyńska, Alina Otlewska, Anna Gawor, Jan Gromadka, Robert Żuchniewicz, Karolina Ziemiński, Krzysztof Microbiologyopen Original Articles This study provides a deep modern insight into the phylogenetic diversity among bacterial consortia found in working and nonworking high‐methane natural gas pipelines located in Poland. The working pipeline was characterized by lower biodiversity (140–154 bacterial genera from 22 to 23 classes, depending on the source of the debris) in comparison to the off‐gas pipeline (169 bacterial genera from 23 classes). The sediment recovered from the working pipeline contained mostly DNA identified as belonging to the phylum Firmicutes (66.4%–45.9% operational taxonomic units [OTUs]), predominantly Bacillus (41.4%–31.1% OTUs) followed by Lysinibacillus (2.6%–1.5% OTUs) and Clostridium (2.4%–1.8% OTUs). In the nonworking pipeline, Proteobacteria (46.8% OTUs) and Cyanobacteria (27.8% OTUs) were dominant. Over 30% of the Proteobacteria sequences showed homologies to Gammaproteobacteria, with Pseudomonas (7.1%), Enhydrobacter (2.1%), Stenotrophomonas (0.5%), and Haempohilus (0.4%) among the others. Differences were noted in terms of the chemical compositions of deposits originating from the working and nonworking gas pipelines. The deposits from the nonworking gas pipeline contained iron, as well as carbon (42.58%), sulphur (15.27%), and oxygen (15.32%). This composition can be linked to both the quantity and type of the resident microorganisms. The presence of a considerable amount of silicon (17.42%), and of aluminum, potassium, calcium, and magnesium at detectable levels, may likewise affect the metabolic activity of the resident consortia in the working gas pipeline. All the analyzed sediments included both bacteria known for causing and intensifying corrosion (e.g., Pseudomonas, Desulfovibrio, Shewanella, Serratia) and bacteria that can protect the surface of pipelines against deterioration (e.g., Bacillus). Biocorrosion is not related to a single mechanism or one species of microorganism, but results from the multidirectional activity of multiple microbial communities. The analysis presented here of the state of the microbiome in a gas pipeline during the real gas transport is a particularly valuable element of this work. John Wiley and Sons Inc. 2019-02-06 /pmc/articles/PMC6692550/ /pubmed/30729757 http://dx.doi.org/10.1002/mbo3.806 Text en © 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Staniszewska, Agnieszka
Kunicka‐Styczyńska, Alina
Otlewska, Anna
Gawor, Jan
Gromadka, Robert
Żuchniewicz, Karolina
Ziemiński, Krzysztof
High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
title High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
title_full High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
title_fullStr High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
title_full_unstemmed High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
title_short High‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
title_sort high‐throughput sequencing approach in analysis of microbial communities colonizing natural gas pipelines
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692550/
https://www.ncbi.nlm.nih.gov/pubmed/30729757
http://dx.doi.org/10.1002/mbo3.806
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