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Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China

BACKGROUND: Highly pathogenic avian influenza (HPAI) A(H7N9) virus emerged and caused human infections during the 2016‐2017 epidemic wave of influenza A(H7N9) viruses in China. We report a human infection with HPAI H7N9 virus and six environmental isolates in Fujian Province, China. METHODS: Environ...

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Autores principales: Yang, Lei, Xie, Jianfeng, Zhang, Ye, Zhu, Wenfei, Li, Xiyan, Wei, Hejiang, Li, Zi, Zhao, Lin, Bo, Hong, Liu, Jia, Dong, Jie, Chen, Tao, Shu, Yuelong, Weng, Yuwei, Wang, Dayan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692551/
https://www.ncbi.nlm.nih.gov/pubmed/31187583
http://dx.doi.org/10.1111/irv.12657
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author Yang, Lei
Xie, Jianfeng
Zhang, Ye
Zhu, Wenfei
Li, Xiyan
Wei, Hejiang
Li, Zi
Zhao, Lin
Bo, Hong
Liu, Jia
Dong, Jie
Chen, Tao
Shu, Yuelong
Weng, Yuwei
Wang, Dayan
author_facet Yang, Lei
Xie, Jianfeng
Zhang, Ye
Zhu, Wenfei
Li, Xiyan
Wei, Hejiang
Li, Zi
Zhao, Lin
Bo, Hong
Liu, Jia
Dong, Jie
Chen, Tao
Shu, Yuelong
Weng, Yuwei
Wang, Dayan
author_sort Yang, Lei
collection PubMed
description BACKGROUND: Highly pathogenic avian influenza (HPAI) A(H7N9) virus emerged and caused human infections during the 2016‐2017 epidemic wave of influenza A(H7N9) viruses in China. We report a human infection with HPAI H7N9 virus and six environmental isolates in Fujian Province, China. METHODS: Environmental surveillance was conducted in live poultry markets and poultry farms in Fujian, China. Clinical and epidemiologic data and samples were collected. Real‐time RT‐PCRs were conducted for each sample, and H7‐positive samples were isolated using embryonated chicken eggs. Full genomes of the isolates were obtained by next‐generation sequencing. Phylogenetic analysis and antigenic analysis were conducted. RESULTS: A 59‐year‐old man who raised about 1000 ducks was identified as HPAI H7N9 infection. Six HPAI H7 viruses were isolated from environmental samples, including five H7N9 viruses and one H7N6 virus. Phylogenetic results showed the human and environmental viruses are highly genetically diverse and containing significantly different gene constellation from that of other HPAI H7N9 previously reported. The internal genes derived from H7N9/H9N2, H5N6, and the Eurasian wild‐bird gene pool, indicating waterfowl‐originated genotypes, have emerged in HPAI H7N9/N6 viruses and caused human infection. CONCLUSION: The new genotypes raise the concern that these HPAI H7 viruses might transmit back into migratory birds and spread to other countries as the HPAI H5Nx viruses. Considering their capability of causing severe infections in both human and poultry, the HPAI H7 viruses in this study pose a risk to public health and the poultry industry and highlight the importance of sustained surveillance of these viruses.
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spelling pubmed-66925512019-09-01 Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China Yang, Lei Xie, Jianfeng Zhang, Ye Zhu, Wenfei Li, Xiyan Wei, Hejiang Li, Zi Zhao, Lin Bo, Hong Liu, Jia Dong, Jie Chen, Tao Shu, Yuelong Weng, Yuwei Wang, Dayan Influenza Other Respir Viruses Original Articles BACKGROUND: Highly pathogenic avian influenza (HPAI) A(H7N9) virus emerged and caused human infections during the 2016‐2017 epidemic wave of influenza A(H7N9) viruses in China. We report a human infection with HPAI H7N9 virus and six environmental isolates in Fujian Province, China. METHODS: Environmental surveillance was conducted in live poultry markets and poultry farms in Fujian, China. Clinical and epidemiologic data and samples were collected. Real‐time RT‐PCRs were conducted for each sample, and H7‐positive samples were isolated using embryonated chicken eggs. Full genomes of the isolates were obtained by next‐generation sequencing. Phylogenetic analysis and antigenic analysis were conducted. RESULTS: A 59‐year‐old man who raised about 1000 ducks was identified as HPAI H7N9 infection. Six HPAI H7 viruses were isolated from environmental samples, including five H7N9 viruses and one H7N6 virus. Phylogenetic results showed the human and environmental viruses are highly genetically diverse and containing significantly different gene constellation from that of other HPAI H7N9 previously reported. The internal genes derived from H7N9/H9N2, H5N6, and the Eurasian wild‐bird gene pool, indicating waterfowl‐originated genotypes, have emerged in HPAI H7N9/N6 viruses and caused human infection. CONCLUSION: The new genotypes raise the concern that these HPAI H7 viruses might transmit back into migratory birds and spread to other countries as the HPAI H5Nx viruses. Considering their capability of causing severe infections in both human and poultry, the HPAI H7 viruses in this study pose a risk to public health and the poultry industry and highlight the importance of sustained surveillance of these viruses. John Wiley and Sons Inc. 2019-06-11 2019-09 /pmc/articles/PMC6692551/ /pubmed/31187583 http://dx.doi.org/10.1111/irv.12657 Text en © 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Yang, Lei
Xie, Jianfeng
Zhang, Ye
Zhu, Wenfei
Li, Xiyan
Wei, Hejiang
Li, Zi
Zhao, Lin
Bo, Hong
Liu, Jia
Dong, Jie
Chen, Tao
Shu, Yuelong
Weng, Yuwei
Wang, Dayan
Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
title Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
title_full Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
title_fullStr Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
title_full_unstemmed Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
title_short Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
title_sort emergence of waterfowl‐originated gene cassettes in hpai h7n9 viruses caused severe human infection in fujian, china
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692551/
https://www.ncbi.nlm.nih.gov/pubmed/31187583
http://dx.doi.org/10.1111/irv.12657
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