Cargando…
Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China
BACKGROUND: Highly pathogenic avian influenza (HPAI) A(H7N9) virus emerged and caused human infections during the 2016‐2017 epidemic wave of influenza A(H7N9) viruses in China. We report a human infection with HPAI H7N9 virus and six environmental isolates in Fujian Province, China. METHODS: Environ...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692551/ https://www.ncbi.nlm.nih.gov/pubmed/31187583 http://dx.doi.org/10.1111/irv.12657 |
_version_ | 1783443578537639936 |
---|---|
author | Yang, Lei Xie, Jianfeng Zhang, Ye Zhu, Wenfei Li, Xiyan Wei, Hejiang Li, Zi Zhao, Lin Bo, Hong Liu, Jia Dong, Jie Chen, Tao Shu, Yuelong Weng, Yuwei Wang, Dayan |
author_facet | Yang, Lei Xie, Jianfeng Zhang, Ye Zhu, Wenfei Li, Xiyan Wei, Hejiang Li, Zi Zhao, Lin Bo, Hong Liu, Jia Dong, Jie Chen, Tao Shu, Yuelong Weng, Yuwei Wang, Dayan |
author_sort | Yang, Lei |
collection | PubMed |
description | BACKGROUND: Highly pathogenic avian influenza (HPAI) A(H7N9) virus emerged and caused human infections during the 2016‐2017 epidemic wave of influenza A(H7N9) viruses in China. We report a human infection with HPAI H7N9 virus and six environmental isolates in Fujian Province, China. METHODS: Environmental surveillance was conducted in live poultry markets and poultry farms in Fujian, China. Clinical and epidemiologic data and samples were collected. Real‐time RT‐PCRs were conducted for each sample, and H7‐positive samples were isolated using embryonated chicken eggs. Full genomes of the isolates were obtained by next‐generation sequencing. Phylogenetic analysis and antigenic analysis were conducted. RESULTS: A 59‐year‐old man who raised about 1000 ducks was identified as HPAI H7N9 infection. Six HPAI H7 viruses were isolated from environmental samples, including five H7N9 viruses and one H7N6 virus. Phylogenetic results showed the human and environmental viruses are highly genetically diverse and containing significantly different gene constellation from that of other HPAI H7N9 previously reported. The internal genes derived from H7N9/H9N2, H5N6, and the Eurasian wild‐bird gene pool, indicating waterfowl‐originated genotypes, have emerged in HPAI H7N9/N6 viruses and caused human infection. CONCLUSION: The new genotypes raise the concern that these HPAI H7 viruses might transmit back into migratory birds and spread to other countries as the HPAI H5Nx viruses. Considering their capability of causing severe infections in both human and poultry, the HPAI H7 viruses in this study pose a risk to public health and the poultry industry and highlight the importance of sustained surveillance of these viruses. |
format | Online Article Text |
id | pubmed-6692551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66925512019-09-01 Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China Yang, Lei Xie, Jianfeng Zhang, Ye Zhu, Wenfei Li, Xiyan Wei, Hejiang Li, Zi Zhao, Lin Bo, Hong Liu, Jia Dong, Jie Chen, Tao Shu, Yuelong Weng, Yuwei Wang, Dayan Influenza Other Respir Viruses Original Articles BACKGROUND: Highly pathogenic avian influenza (HPAI) A(H7N9) virus emerged and caused human infections during the 2016‐2017 epidemic wave of influenza A(H7N9) viruses in China. We report a human infection with HPAI H7N9 virus and six environmental isolates in Fujian Province, China. METHODS: Environmental surveillance was conducted in live poultry markets and poultry farms in Fujian, China. Clinical and epidemiologic data and samples were collected. Real‐time RT‐PCRs were conducted for each sample, and H7‐positive samples were isolated using embryonated chicken eggs. Full genomes of the isolates were obtained by next‐generation sequencing. Phylogenetic analysis and antigenic analysis were conducted. RESULTS: A 59‐year‐old man who raised about 1000 ducks was identified as HPAI H7N9 infection. Six HPAI H7 viruses were isolated from environmental samples, including five H7N9 viruses and one H7N6 virus. Phylogenetic results showed the human and environmental viruses are highly genetically diverse and containing significantly different gene constellation from that of other HPAI H7N9 previously reported. The internal genes derived from H7N9/H9N2, H5N6, and the Eurasian wild‐bird gene pool, indicating waterfowl‐originated genotypes, have emerged in HPAI H7N9/N6 viruses and caused human infection. CONCLUSION: The new genotypes raise the concern that these HPAI H7 viruses might transmit back into migratory birds and spread to other countries as the HPAI H5Nx viruses. Considering their capability of causing severe infections in both human and poultry, the HPAI H7 viruses in this study pose a risk to public health and the poultry industry and highlight the importance of sustained surveillance of these viruses. John Wiley and Sons Inc. 2019-06-11 2019-09 /pmc/articles/PMC6692551/ /pubmed/31187583 http://dx.doi.org/10.1111/irv.12657 Text en © 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Yang, Lei Xie, Jianfeng Zhang, Ye Zhu, Wenfei Li, Xiyan Wei, Hejiang Li, Zi Zhao, Lin Bo, Hong Liu, Jia Dong, Jie Chen, Tao Shu, Yuelong Weng, Yuwei Wang, Dayan Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China |
title | Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China |
title_full | Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China |
title_fullStr | Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China |
title_full_unstemmed | Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China |
title_short | Emergence of waterfowl‐originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China |
title_sort | emergence of waterfowl‐originated gene cassettes in hpai h7n9 viruses caused severe human infection in fujian, china |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692551/ https://www.ncbi.nlm.nih.gov/pubmed/31187583 http://dx.doi.org/10.1111/irv.12657 |
work_keys_str_mv | AT yanglei emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT xiejianfeng emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT zhangye emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT zhuwenfei emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT lixiyan emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT weihejiang emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT lizi emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT zhaolin emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT bohong emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT liujia emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT dongjie emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT chentao emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT shuyuelong emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT wengyuwei emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina AT wangdayan emergenceofwaterfowloriginatedgenecassettesinhpaih7n9virusescausedseverehumaninfectioninfujianchina |