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Comparison of the ligand‐binding properties of fluorescent VEGF‐A isoforms to VEGF receptor 2 in living cells and membrane preparations using NanoBRET

BACKGROUND AND PURPOSE: Vascular endothelial growth factor A (VEGF‐A) is a key mediator of angiogenesis. A striking feature of the binding of a fluorescent analogue of VEGF(165)a to nanoluciferase‐tagged VEGF receptor 2 (VEGFR2) in living cells is that the BRET signal is not sustained and declines o...

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Detalles Bibliográficos
Autores principales:	Peach, Chloe J., Kilpatrick, Laura E., Woolard, Jeanette, Hill, Stephen J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2019
Materias:	Research Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692582/ https://www.ncbi.nlm.nih.gov/pubmed/31162634 http://dx.doi.org/10.1111/bph.14755

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