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CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665
Background: Numerous studies have shown that circular RNAs (circRNAs) play vital roles in tumor progression. However, how circRNAs function in hepatocellular cancer (HCC) remains mostly unclear. Methods: We analyzed HCC circRNA expression via a microarray, and the expression of an upregulated circRN...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692622/ https://www.ncbi.nlm.nih.gov/pubmed/31417632 http://dx.doi.org/10.7150/jca.31362 |
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author | Bai, Ning Peng, Eming Xia, Fada Wang, Dong Li, Xiaogang Li, Xinying |
author_facet | Bai, Ning Peng, Eming Xia, Fada Wang, Dong Li, Xiaogang Li, Xinying |
author_sort | Bai, Ning |
collection | PubMed |
description | Background: Numerous studies have shown that circular RNAs (circRNAs) play vital roles in tumor progression. However, how circRNAs function in hepatocellular cancer (HCC) remains mostly unclear. Methods: We analyzed HCC circRNA expression via a microarray, and the expression of an upregulated circRNA, circABCC2, was detected. We next explored the function of circABCC2 in HCC via a series of experiments. We performed RNA immunoprecipitation (RIP) and luciferase assays to explore the competing endogenous RNA (ceRNA) function of circABCC2 in HCC. Results: qRT-PCR verified that circABCC2 was overexpressed in HCC. Inhibition of circABCC2 suppressed HCC cell proliferation and invasion, but promoted apoptosis. Luciferase assays and RIP showed that circABCC2 and ABCC2 could directly bind to miR-665 and that circABCC2 could regulate ABCC2 expression by sponging miR-665. Conclusions: In summary, circABCC2 regulates ABCC2 expression and HCC progression by sponging miR-665. circABCC2 could be used as a biomarker and therapeutic target in HCC. |
format | Online Article Text |
id | pubmed-6692622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-66926222019-08-15 CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 Bai, Ning Peng, Eming Xia, Fada Wang, Dong Li, Xiaogang Li, Xinying J Cancer Research Paper Background: Numerous studies have shown that circular RNAs (circRNAs) play vital roles in tumor progression. However, how circRNAs function in hepatocellular cancer (HCC) remains mostly unclear. Methods: We analyzed HCC circRNA expression via a microarray, and the expression of an upregulated circRNA, circABCC2, was detected. We next explored the function of circABCC2 in HCC via a series of experiments. We performed RNA immunoprecipitation (RIP) and luciferase assays to explore the competing endogenous RNA (ceRNA) function of circABCC2 in HCC. Results: qRT-PCR verified that circABCC2 was overexpressed in HCC. Inhibition of circABCC2 suppressed HCC cell proliferation and invasion, but promoted apoptosis. Luciferase assays and RIP showed that circABCC2 and ABCC2 could directly bind to miR-665 and that circABCC2 could regulate ABCC2 expression by sponging miR-665. Conclusions: In summary, circABCC2 regulates ABCC2 expression and HCC progression by sponging miR-665. circABCC2 could be used as a biomarker and therapeutic target in HCC. Ivyspring International Publisher 2019-06-10 /pmc/articles/PMC6692622/ /pubmed/31417632 http://dx.doi.org/10.7150/jca.31362 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Bai, Ning Peng, Eming Xia, Fada Wang, Dong Li, Xiaogang Li, Xinying CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 |
title | CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 |
title_full | CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 |
title_fullStr | CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 |
title_full_unstemmed | CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 |
title_short | CircABCC2 Regulates Hepatocellular Cancer Progression by Decoying MiR-665 |
title_sort | circabcc2 regulates hepatocellular cancer progression by decoying mir-665 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692622/ https://www.ncbi.nlm.nih.gov/pubmed/31417632 http://dx.doi.org/10.7150/jca.31362 |
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