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Synthetically Scalable Poly(ampholyte) Which Dramatically Enhances Cellular Cryopreservation
[Image: see text] The storage and transport of frozen cells underpin the emerging/existing cell-based therapies and are used in every biomedical research lab globally. The current gold-standard cryoprotectant dimethyl sulfoxide (DMSO) does not give quantitative cell recovery in suspension or in two-...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692820/ https://www.ncbi.nlm.nih.gov/pubmed/31268698 http://dx.doi.org/10.1021/acs.biomac.9b00681 |
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author | Bailey, Trisha L. Stubbs, Christopher Murray, Kathryn Tomás, Ruben M. F. Otten, Lucienne Gibson, Matthew I. |
author_facet | Bailey, Trisha L. Stubbs, Christopher Murray, Kathryn Tomás, Ruben M. F. Otten, Lucienne Gibson, Matthew I. |
author_sort | Bailey, Trisha L. |
collection | PubMed |
description | [Image: see text] The storage and transport of frozen cells underpin the emerging/existing cell-based therapies and are used in every biomedical research lab globally. The current gold-standard cryoprotectant dimethyl sulfoxide (DMSO) does not give quantitative cell recovery in suspension or in two-dimensional (2D) or three-dimensional (3D) cell models, and the solvent and cell debris must be removed prior to application/transfusion. There is a real need to improve this 50-year-old method to underpin emerging regenerative and cell-based therapies. Here, we introduce a potent and synthetically scalable polymeric cryopreservation enhancer which is easily obtained in a single step from a low cost and biocompatible precursor, poly(methyl vinyl ether-alt-maleic anhydride). This poly(ampholyte) enables post-thaw recoveries of up to 88% for a 2D cell monolayer model compared to just 24% using conventional DMSO cryopreservation. The poly(ampholyte) also enables reduction of [DMSO] from 10 wt % to just 2.5 wt % in suspension cryopreservation, which can reduce the negative side effects and speed up post-thaw processing. After thawing, the cells have reduced membrane damage and faster growth rates compared to those without the polymer. The polymer appears to function by a unique extracellular mechanism by stabilization of the cell membrane, rather than by modulation of ice formation and growth. This new macromolecular cryoprotectant will find applications across basic and translational biomedical science and may improve the cold chain for cell-based therapies. |
format | Online Article Text |
id | pubmed-6692820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-66928202019-08-15 Synthetically Scalable Poly(ampholyte) Which Dramatically Enhances Cellular Cryopreservation Bailey, Trisha L. Stubbs, Christopher Murray, Kathryn Tomás, Ruben M. F. Otten, Lucienne Gibson, Matthew I. Biomacromolecules [Image: see text] The storage and transport of frozen cells underpin the emerging/existing cell-based therapies and are used in every biomedical research lab globally. The current gold-standard cryoprotectant dimethyl sulfoxide (DMSO) does not give quantitative cell recovery in suspension or in two-dimensional (2D) or three-dimensional (3D) cell models, and the solvent and cell debris must be removed prior to application/transfusion. There is a real need to improve this 50-year-old method to underpin emerging regenerative and cell-based therapies. Here, we introduce a potent and synthetically scalable polymeric cryopreservation enhancer which is easily obtained in a single step from a low cost and biocompatible precursor, poly(methyl vinyl ether-alt-maleic anhydride). This poly(ampholyte) enables post-thaw recoveries of up to 88% for a 2D cell monolayer model compared to just 24% using conventional DMSO cryopreservation. The poly(ampholyte) also enables reduction of [DMSO] from 10 wt % to just 2.5 wt % in suspension cryopreservation, which can reduce the negative side effects and speed up post-thaw processing. After thawing, the cells have reduced membrane damage and faster growth rates compared to those without the polymer. The polymer appears to function by a unique extracellular mechanism by stabilization of the cell membrane, rather than by modulation of ice formation and growth. This new macromolecular cryoprotectant will find applications across basic and translational biomedical science and may improve the cold chain for cell-based therapies. American Chemical Society 2019-06-20 2019-08-12 /pmc/articles/PMC6692820/ /pubmed/31268698 http://dx.doi.org/10.1021/acs.biomac.9b00681 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Bailey, Trisha L. Stubbs, Christopher Murray, Kathryn Tomás, Ruben M. F. Otten, Lucienne Gibson, Matthew I. Synthetically Scalable Poly(ampholyte) Which Dramatically Enhances Cellular Cryopreservation |
title | Synthetically Scalable Poly(ampholyte) Which Dramatically
Enhances Cellular Cryopreservation |
title_full | Synthetically Scalable Poly(ampholyte) Which Dramatically
Enhances Cellular Cryopreservation |
title_fullStr | Synthetically Scalable Poly(ampholyte) Which Dramatically
Enhances Cellular Cryopreservation |
title_full_unstemmed | Synthetically Scalable Poly(ampholyte) Which Dramatically
Enhances Cellular Cryopreservation |
title_short | Synthetically Scalable Poly(ampholyte) Which Dramatically
Enhances Cellular Cryopreservation |
title_sort | synthetically scalable poly(ampholyte) which dramatically
enhances cellular cryopreservation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692820/ https://www.ncbi.nlm.nih.gov/pubmed/31268698 http://dx.doi.org/10.1021/acs.biomac.9b00681 |
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