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GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton

BACKGROUND: The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the functio...

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Autores principales: He, Peng, Wu, Shuyin, Jiang, Yanli, Zhang, Lihua, Tang, Meiju, Xiao, Guanghui, Yu, Jianing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693126/
https://www.ncbi.nlm.nih.gov/pubmed/31409298
http://dx.doi.org/10.1186/s12870-019-1945-1
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author He, Peng
Wu, Shuyin
Jiang, Yanli
Zhang, Lihua
Tang, Meiju
Xiao, Guanghui
Yu, Jianing
author_facet He, Peng
Wu, Shuyin
Jiang, Yanli
Zhang, Lihua
Tang, Meiju
Xiao, Guanghui
Yu, Jianing
author_sort He, Peng
collection PubMed
description BACKGROUND: The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. RESULTS: In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. CONCLUSIONS: Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1945-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-66931262019-08-16 GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton He, Peng Wu, Shuyin Jiang, Yanli Zhang, Lihua Tang, Meiju Xiao, Guanghui Yu, Jianing BMC Plant Biol Research Article BACKGROUND: The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. RESULTS: In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. CONCLUSIONS: Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1945-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-08-13 /pmc/articles/PMC6693126/ /pubmed/31409298 http://dx.doi.org/10.1186/s12870-019-1945-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
He, Peng
Wu, Shuyin
Jiang, Yanli
Zhang, Lihua
Tang, Meiju
Xiao, Guanghui
Yu, Jianing
GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
title GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
title_full GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
title_fullStr GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
title_full_unstemmed GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
title_short GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
title_sort ghygl1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693126/
https://www.ncbi.nlm.nih.gov/pubmed/31409298
http://dx.doi.org/10.1186/s12870-019-1945-1
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